Shale gas exploratory development requires a huge water source for hydraulic fracturing, but the well sites are mainly located in hills, which limits the industrialization of shale gas development. The CO2 foaming fluid can meet the requirements of fracturing fluid in fracking and relieve pressure on water resources. The friction calculation of the CO2 foaming hydro-fracturing fluid in the conduit flow is key to the reliability of the overall fracture engineering, which affects the formation stimulation effect. The friction properties of CO₂ foaming hydro-fracturing fluids were investigated by conducting flow experiments in HTHP conditions, and the influence of pressure, temperature, foam mass, and flow rate on the friction properties was obtained. Mathematical models of frictional drag coefficients have been developed to predict the frictional force of CO2 foaming gel hydro-fracturing fluids in turbulent flows, which can be useful in evaluating the rating of CO2 foaming hydro-fracturing designs.

Serial VPO catalysts with different crystal structures were prepared by adjusting ratios of air and N2 in calcination process. The results showed that, as the air and N2 ratios were lower than 1:1, the ordered VOHPO4•0.5H2O phase was mainly formed in precursors during calcination process. However, as the ratio of air and N2 reached 3:1, the amorphous VOPO4 phases would be formed over the surface of precursor during calcination process, which would prevent the formation of ordered V5+ species over the surface of VPO catalyst after activation. The evaluation results of n-butane oxidation showed that, as the ratio of air and N2 was 1:3, the corresponding VPO-1A catalyst presented the highest conversion of n-butane and yield of MA product (57.6 m% at 412 °C). Finally, a kinetic and thermodynamic model was established and calculated to investigate the characteristics of n-butane oxidation reactions for various VPO catalysts.

Melamine production is known to produce tail gas with a significant amount of CO2 and NH3. Ionic liquid as a green and excellent solvent is to be used for separating melamine tail gas. This paper aims to present a detailed multi-objective optimization (MOO) for the CO2/NH3 separation of the tail gas by ionic liquid (IL) in technical-energy-economic evaluation. Functional IL ([Bim][NTf2]) was selected, the ionic liquid-based process was designed, and process performances were investigated by process simulation and optimization. According to the optimization results, specific cases with different objective functions were chosen, and the relationship between key parameters was analyzed. The results showed that the ionic liquid-based technology could realize a better performance regarding the technical-energy-economic than the base case. Deeper analyses with the influence of operation parameters with evaluation index were carried out, thus providing support to the industrial application of the new separation technology.

Natural gas hydrates of Bulgaria and Romania in the Black Sea have been subject to studies by several European research projects. In this study, we have evaluated some well-known hydrate production methods. Thermal stimulation and adding chemicals are considered as not economically feasible. Pressure reduction may not be efficient due to the endothermic dissociation of hydrates and long-term cooling of the sediments. Adding CO2/N2, however, has a dual value. In the future CO2 can be stored at a price proportional to a CO2 tax. The maximum N2 amount is dictated by the demand for the creation of a new hydrate from injection gas but also the need for sufficient heat release from this hydrate formation to dissociate the in situ CH4 hydrates. Based on reasonable assumptions and approximations as used in a verified kinetic model it is found that CH4/CO2 swapping is a feasible method for Black Sea hydrates.

As key components of antifouling material surfaces, the design and screening of polymer molecules grafted on the substrate are critical. However, current experimental and computational models still retain an empirical flavor due to the complex structure of polymers. Here, we report a simple and general strategy that enables multi-scale design and screening of easily synthesized functional polymer molecules to address this challenge. Specifically, the required functions of the antifouling material are decomposed and assigned to different modules of the polymer molecules. By designing different modules, a novel bio-inspired polymer with three zwitterionic poly (sulfobetaine methacrylate) (PSBMA) chains, three catechol (DOPA) anchors (tri-DOPA-PSBMA), and a tris(2-aminoethyl) amine (TREN) scaffold were screened out. Moreover, it was successfully synthesized via an atom transfer radical polymerization (ATRP). The excellent performance of tri-DOPA-PSBMA with a versatile and convenient grafting strategy makes it a promising material for marine devices, biomedical devices, and industrial applications.

Perceived high risk of COVID-19 vaccination: the revealing power of placebo.Beyens Michiel1,2; Toscano Alessandro1,2; Van Damme Pierre3; Dogné Jean-Michel4; Ebo Didier1,2,5*; Sabato Vito1,2,51 Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium2 Immunology, Allergology, Rheumatology, Antwerp University Hospital, Antwerp, Belgium3 Centre for the Evaluation of Vaccination and Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.4 Department of Pharmacy, Namur Research Institute for LIfe Sciences, University of Namur, Namur, Belgium.5 Immunology and Allergology, AZ Jan Palfijn Gent, Ghent, Belgium

In this work, atomic connectivity group contribution (ACGC) method is developed for predicting critical properties of organic compounds. Herein, a new group defining method, namely atomic adjacent group (AAG) method, is proposed to describe the relationship between core atom and its adjacent atoms. For distinguishing isomers effectively, the shape factor (SF) is used to describe the effect of molecular shape on group, and atomic connectivity factors (ACF) are defined for describing the position of each group in a molecule. The external and internal verification methods were utilized during the modelling process. Compared with AAG model, ARE decreased by 6.82-42.57 % when SF was considered and, ARE decreased by 24.19-62.25 % when both SF and ACF were applied as using the ACGC method. Accordingly, SF and ACF are effective in improving the group contribution method and ACGC method is accurate in calculating the properties of organic compounds.

With the rapid expansion of food trade, there is a growing concern about health issues. As a result, consumer demand for high quality food is increasing and food quality analysis has become a very important and interesting area of research. The application of biosensors in food analysis is promising because specific biosensors can be used to easily access the nutritional composition inside food products, including macronutrients, trace elements and other bioactive substances. Deep learning includes many different types of artificial neural networks, and convolutional neural networks can be used to extract external features of food products from images, such as shape, size, color, etc. Deep neural networks are able to generate predictive models using different food properties. In this paper, we aim to show how to combine biosensors and deep learning to assess food quality. We first focus on the process of generating predictive models by deep neural networks and the datasets required to train the models. Secondly, we focus on how to use convolutional neural networks to extract external features of food products and representative research work on biosensors for food nutrient content analysis. Finally, the paper summarizes and looks at the challenges and possible solutions of the approach in the field of food quality assessment.

Background: Clinical trials evaluating immune checkpoint inhibition (ICI) in recurrent high-grade gliomas (rHGG) report 7-20% 6-month progression-free survival (PFS), while re-irradiation demonstrates 28%-39% 6-month PFS. Aims: We evaluate outcomes of patients treated with ICI and concurrent re-irradiation utilizing stereotactic body radiotherapy / fractionated stereotactic radiosurgery (SBRT) compared to ICI monotherapy. Methods and Results: Patients >18-years-old with rHGG (WHO grade III and IV) receiving ICI+SBRT or ICI monotherapy between 1/1/16-1/1/19 were included. Adverse events, 6-month PFS and overall survival (OS) were assessed. Log-rank tests were used to evaluate PFS and OS. Histogram analyses of apparent diffusion coefficient maps and dynamic contrast-enhanced magnetic resonance perfusion metrics were performed. Twenty-one patients with rHGG (ICI+SBRT: 16; ICI: 5) were included. The ICI+SBRT and ICI groups received a mean 7.25 and 6.2 ICI cycles, respectively. There were five grade 1, one grade 2 and no grade 3-5 AEs in the ICI+SBRT group, and four grade 1 and no grade 2-5 AEs in the ICI group. Median PFS was 2.85 and 1 month for the ICI+SBRT and ICI groups; median OS was 7 and 6 months among ICI+SBRT and ICI groups, respectively. There were significant differences in pre- and post-treatment tumor volume in the cohort (12.35 vs. 20.51; p=0.03), but not between treatment groups. Conclusions: In this heavily pretreated cohort, ICI with re-irradiation utilizing SBRT was well tolerated. Prospective studies are warranted to evaluate potential therapeutic benefits to re-irradiation with ICI+SBRT in rHGG.

Five minutes for a life is an innovative program designed in Mexico consisting of four main axes: Educational strategy, Cancer Booklet, National Health Law related to white eye search, and measurement of Indicators through research. The strategy is divided into five tumors that have the characteristic of presenting symptoms in their initial stages that can be detected by a non-specialist physician without specialized laboratory or studies. In addition, if treated in early stages, have cure rates of over 80% or avoid mutilation of an organ, in this case the eyes or limbs.

Background: Human papillomavirus (HPV) is the causative agent of nearly all forms of cervical cancer, which can arise upon viral integration into the host genome and concurrent loss of viral regulatory gene E2. Gene-based delivery approaches show that E2 reintroduction reduces proliferative capacity and promotes apoptosis in vitro. This work explored if our calcium-dependent protein-based delivery system, TAT-CaM could deliver functional E2 protein directly into cervical cancer cells to limit proliferative capacity and induce cell death. Methods: TAT-CaM and the HPV16 E2 protein containing a CaM-binding sequence (CBS-E2) were expressed and purified from E. coli. Calcium-dependent binding kinetics were verified by Biolayer Interferometry. Equimolar TaT-CaM:CBS-E2 constructs were delivered into the HPV16+ SiHa cell line and uptake verified by confocal microscopy. Proliferative capacity was measured by MTS assay and cell death was measured by release of lactate dehydrogenase. As a control for specificity to HPV+ cells, human microvascular cells (HMECs) were used. Results: TAT-CaM bound CBS-E2 with high affinity in the presence of calcium and rapidly disassociated in its absence. After introduction by TAT-CaM, E2 was detected in cellular interiors by orthogonal projects taken at the depth of the nucleus. In dividing cells, E2 relocalized to regions associated with the mitotic spindle. Cells receiving a single daily dose of CBS-E2 for 4 days showed a significant reduction in metabolic activity at low doses and cell death at high doses compared to controls. This phenotype was retained for 7 days with no further treatments. When subcultured at day 12, treated cells regained their proliferative capacity. Conclusions: Using the TAT-CaM platform, bioactive E2 protein was delivered into living cervical cancer cells, inducing senescence and cell death in a time- and dose-dependent manner. These results suggest that this nucleic acid and virus-free delivery method could be harnessed to develop novel, effective protein therapeutics.

Background Multiple myeloma (MM) patients have variable responses to mRNA vaccination to COVID-19. Little is known regarding their vaccine-induced antibody levels over time. Methods We monitored spike IgG antibody levels over 24 weeks among a subset of 18 MM patients who showed a full response after two mRNA vaccinations. MM patients had a more rapid decline in antibody levels as compared to 8 healthy controls, with power law half-lives of 72 days (versus 107 days) and exponential half-lives of 37 days (versus 51 days). Results The patients with longer SARS-CoV-2 antibody half-lives were more likely to have undetectable monoclonal protein than those with shorter half-lives, suggesting better disease control may correlate with longer duration of vaccine-induced antibodies. Regardless, by 16 weeks post-second dose of mRNA vaccination, the majority of patients had antibody levels below 250 binding arbitrary units per milliliter, which would be unlikely to contribute significantly to preventing COVID-19. Conclusions Thus, even MM patients who respond adequately to vaccination are likely to require more frequent booster doses than the general population.

Social touch is closely related to the establishment and maintenance of social bonds in humans and the sensory brain circuit for gentle brushing is already active soon after birth. Brain development is known to be sexually dimorphic, but the potential effect of sex on brain activation to gentle touch remains unknown. Here, we examined brain activation to gentle skin stroking, a tactile stimulation that resembles affective/social touch, in term-born neonates. Eighteen infants aged 11-36 days, recruited from the FinnBrain Birth Cohort Study, were included in the study. During natural sleep, soft brush strokes were applied to the skin of the right leg during functional magnetic resonance imaging (fMRI) at 3cm/s velocity. We examined potential differences in brain activation between males (n = 10) and females (n = 8) and found that females had larger blood oxygenation level dependent (BOLD) responses (brushing vs. rest) in bilateral orbitofrontal cortex (OFC), right ventral striatum and bilateral inferior striatum, pons and cerebellum compared to males. Moreover, the psychophysiological interactions (PPI) analysis, setting left and right OFC as seed regions, revealed significant differences between males and females. Females exhibited stronger PPI connectivity between the left OFC and posterior cingulate/cuneus. Our work suggests that social touch neural responses are different in male and female neonates, which may have major ramifications for later brain, cognitive and social development. Finally, many of the sexually dimorphic brain responses were subcortical, not captured by surface-based neuroimaging, indicating that fMRI will be a relevant technique for future studies.

Social communication draws on several cognitive functions such as perception, emotion recognition and attention. In a previous study, we demonstrated that macaques associate audio-visual information when processing their species-specific communicative signals. Specifically, cortical activation is inhibited when there is a mismatch between vocalisations and social visual information whereas activation is enhanced in the lateral sulcus, superior temporal sulcus as well as a larger network composed of early visual and prefrontal areas when vocalisations and social visual information match. Here, we use a similar task and functional magnetic resonance imaging to assess the role of subcortical structures. We identify three subcortical regions involved in audio-visual processing of species-specific communicative signal: the amygdala, the claustrum and the pulvinar. Like the cortex, these subcortical structures are not activated when there is a mismatch between visual and acoustic information. In contrast, the amygdala and claustrum are activated by visual, auditory congruent and audio-visual stimulations. The pulvinar responds in a task-dependent manner, along a specific spatial sensory gradient. Anterior pulvinar responds to auditory stimuli, medial pulvinar is activated by auditory, audio-visual and visual stimuli and the dorsal lateral pulvinar only responds to visual stimuli in a pure visual task. The medial pulvinar and the amygdala are the only subcortical structures integrating audio-visual social stimuli. We propose that these three structures belong to a multisensory network that modulates the perception of visual socioemotional information and vocalizations as a function of the relevance of the stimuli in the social context.

Objective: There has been a rise in the prenatal detection of right sided aortic arch (RAA). When associated with a left-sided arterial duct (LD), this forms a vascular ring encircling the trachea. Infants may have symptoms or signs suggestive of trache-oesophageal compression but many are asymptomatic. The objective of this study was to investigate the relationship between symptoms and severity of tracheo-bronchial compression assessed by bronchoscopy. Methods: Retrospective review of all cases of prenatally diagnosed RAA-LD in the absence of associated congenital heart disease at Evelina London Children’s Hospital and Kings College Hospital over a 4-year period between April 2015-2019. Clinical records, fetal echocardiograms and free-breathing flexible bronchoscopy (FB) data were reviewed. Results: 112 cases of isolated RAA-LD were identified of whom 82 cases (73%) underwent FB. FB was performed median age of 11 months (range 1-36 months), no complications occurred. Aberrant left subclavian artery (ALSA) was present in 86% (96/112) and mirror image branching (MIB) in 13% (15/112). 34/112 (30%) reported symptoms during follow up. 36/77 (47%) with ALSA who underwent FB showed moderate-severe compression mostly at distal tracheal and carinal level of whom 38% had parent-reported symptoms. Moderate-severe compression was seen in 3/5 (60%) with MIB mostly at mid tracheal level of whom 3 were symptomatic but only 2 with compression. In total 36% (18/50) of investigated asymptomatic patients showed moderate to severe compression. Respiratory symptoms were poorly predictive of moderate-severe compression (positive predictive value 66%). Conclusion: The absence of symptoms did not exclude significant tracheal compression. The anatomical effect of the vascular ring is underappreciated when symptoms alone are used as a marker of tracheal compression.

The incidence rate of vaccine induced autoimmune disease is unknown, but frequently reported. Here I report my own mRNA vaccine induced autoimmune disease that has been mitigated by a diet strategy, and reference several other cases, including a life threatening episode treated with a polypharmacy approach. I discuss the mechanisms by which a whole food plant based diet may mitigate autoimmunity, including the mitigation of autoantibodies that target neural tissue and likely cause many of the symptoms.

Landscape features are effective geographical barriers resulting inpopulation differentiation of plant species. Taihang Mountains in China possess complexly geographical topology and specific landscape characteristics. Two closely related syntopic Opisthopappus species, mainly distribute in different areas of Taihang Mountains, respectively. How the landscape of Taihang Mountains affects the gene exchange between these two species still unclear. Combined SNP data from restriction-site associated DNA sequencing (RAD-seq) and recently developed landscape genetic methods (EEMS, Samβada, LFMM), we conducted a landscape genetic analysis of these two species. It found that the diversity of O. longilobus was higher than that of O. taihangensis, the gene flow was mostly from north to south along Taihang Mountains. However, a general north–south gene exchange barrier between O. longilobus and O. taihangensis was detected. Among the landscape factors of Taihang Mountains, eight was found to be the important ones, including average precipitation in August, October, and November, solar radiation in August, soil PH, built-up land, rain-fed cultivated land, and workability. And these eight factors were closely related to the occurred barriers, indicating that climatic conditions and human activities rather than geographical environment resulted in these barriers. Twenty-nine selected SNPs were identified to be significant correlated with the eight factors, especially average precipitation in November. Thus, the average precipitation in November could be regarded as an ecological indicator for O. longilobus and O. taihangensis. The results revealed the effect of landscape features on two species and the adaption on the landscape environment of Taihang Mountains during the long-term evolution.

 Introduction: Diabetes being one of the most common comorbidities of COVID-19, is prioritized for SARS-CoV-2 vaccination. To evaluate the T-cell mediated immune responses in T2DM, this study assessed and compared IFN-\(\gamma\) and IL-2 secreting PBMCs against the SARS-CoV-2 BNT162b2 mRNA vaccine. Methods: 19 known cases of type 2 diabetes and 16 healthy controls were included through purposive sampling and were regularly sampled before 1st dose, after 1st and 2nd dose of vaccination. Ex-vivo ELISpot assays for IFN-\(\gamma\) and IL-2 were done using isolated PBMCs along with HbA1c status and SARS-CoV-2 spike-specific IgG antibody. A p-value < 0.05 was considered significant. Results: All participants were seroconverted after 1st dose and showed a robust rise of IFN-\(\gamma\) and IL-2 secreting PBMCs counts in T2DM participants irrespective of hypertension, gender and age after 2 doses of vaccination. However, IFN-\(\gamma\) secreting PBMCs counts were significantly lower in type 2 diabetic group than healthy control group after 1st dose. Conclusion: Although type 2 diabetic participants had a lower response initially, BNT162b2 mRNA vaccine overall induces a robust T-cell mediated immune response  

Objective To systematically explore the association between pathogenic/likely pathogenic copy number variations (pCNV) and ultrasonographic anomalies and soft markers. Design Retrospective cohort study. Setting Data were obtained from multiple centers in china. Population or Sample Fetuses performed low-pass genome sequencing and ultrasonography between 2016 and 2020. Method The yields of pCNV under various ultrasonographic indications were compared with that of fetuses with no identifiable anomalies. In addition, the ultrasonographic characteristics of aneuploidy and pCNV were described in comparison with those of fetuses without chromosomal aberrations. Main Outcome Measures Yields of aneuploidy and pCNV in different ultrasonographic indications. Results Ten of the 12 ultrasonographic anomalies had significantly higher yield of pCNV, except for fetal hydrops and abnormal amniotic fluid, of which the gastrointestinal, facial, respiratory systems, and abdominal wall defect were rarely reported. Similarly, five of the 12 soft markers had significantly higher yield of pCNV, with single umbilical artery being rarely reported. Furthermore, this study reported that four duplications/deletions were associated with novel ultrasonographic findings. Conclusions Based on specific ultrasonographic phenotypes, prenatal genetic testing could be considered in a tailored fashion. Keywords Low-pass genome sequencing; ultrasonographic anomaly; soft marker; copy number variations; aneuploidy; prenatal diagnosis Tweetable abstract Fetuses with structural anomalies and specific soft markers are recommended for copy number variations analysis

OBJECTIVE To assess the relationship between allostatic load in early pregnancy and CVD, 2 to 7 years postpartum, and potential pathways contributing to racial disparities in CVDs. DESIGN Secondary analysis of an observational cohort study. SETTING nuMom2b Heart Health Study. POPULATION Pregnant individuals. METHODS Our primary exposure was dichotomous high allostatic load in the first trimester, defined as four or more out of 12 biomarkers in the “worst” quartile. The primary outcome was new diagnosis of composite CVD, consisting of HTN and or MD (fasting glucose greater than 100 mg/dL or medication for diabetes). Each outcome and allostatic load component was analyzed secondarily. Multivariable logistic regression was used to test the association between high allostatic load and CVD adjusted for potential confounders. Mediation and moderation analyses assessed the role of high allostatic load in racial disparities of CVD. MAIN OUTCOME MEASURE Composite CVD. RESULTS Among 4,022 individuals, CVD was identified in 1,462 (36.4%); 26.6% had HTN, and had 15.4% MD. High allostatic load was present in 33.0%. After adjustment for covariates, high allostatic load was associated with CVD (aOR 2.0, 1.8-2.3), HTN (2.1, 1.8-2.4), and MD (1.7, 1.5-2.1). There was a reduction in the magnitude of the relationship between race and CVD with the addition of allostatic load. Self-reported race did not significantly moderate the relationship between allostatic load and CVD. CONCLUSION High allostatic load is associated with CVD. Allostatic load was a partial mediator between race and CVD. Race did not moderate the relationship between allostatic load and CVD.

Urethral diverticula have been described in other species. Congenital anomalies of the urinary tract in horses are rare. A 26-year-old gelding presented with complaints of abdominal discomfort and dysuria. Urine dribbling was reported since several years. General clinical examination was within normal limits. Rectal palpation showed a distended bladder, which was emptied spontaneously after manipulation. Urine cytology showed an increased white blood cell count and presence of bacteria. Bacterial culture however remained sterile. Cystoscopy identified two symmetrical urethral diverticula dorsal to the entrance of the bladder, lined with normal epithelium. Mucosa of the bladder, urethra and two diverticula were hyperemic with evidence of urine accumulation in both diverticula. Ultrasound, abdominal and transrectal, did not identify the diverticula. Treatment included trimethoprim-sulfamides and meloxicam. After 2 weeks, the owner reported resolution of clinical signs. It is unclear if the diverticula in this case were congenital or acquired. In humans it is described that urethral diverticula can be asymptomatic and incidental findings. Surgical intervention has been described in small animal and human medicine with complete resolution of clinical signs, however in this case was not possible due to financial restraints.

Mapping the spatial and temporal dynamics of species distributions is necessary for biodiversity conservation land-use planning decisions. Recent advances in remote sensing and machine learning have allowed for high resolution species distribution modelling that can inform landscape-level decision making. Here we compare the performance of three popular Sentinel-2 (10m) land cover maps including Dynamic World (DW), European land cover (ELC10) and World Cover (WC), in predicting wild bee species richness over southern Norway. The proportion of grassland habitat within 250m (derived from the land cover maps), along with temperature and distance to sandy soils, were used as predictors in both Bayesian Regularized Neural Network and Random Forest models. Models using grassland habitat from DW performed best (RMSE = 2.85; averaged across models), followed by WC (RMSE = 2.86) and ELC10 (RMSE = 2.89). All satellite-derived maps outperformed a manually mapped Norwegian land cover dataset called AR5 (RMSE = 3.02). When validating the model predictions of bee species richness against citizen science data on solitary bee occurrences using generalized linear models, we found that ELC10 performed best (AIC = 2800), followed by WC (AIC = 2939), and DW (AIC = 2973). While the differences in RMSE we observed between models were small, they may be significant when such models are used to prioritize grassland patches within a landscape for conservation subsidies or management policies. Partial dependencies in our models showed that increasing the proportion of grassland habitat is positively associated with wild bee species richness, thereby justifying bee conservation schemes that aim to enhance semi-natural grassland habitat. Our results confirm the utility of satellite-derived land cover maps in supporting high resolution species distribution modelling and suggest there is scope to monitor changes in species distributions over time given the dense time series provided by products like DW.

Digital technologies (DTs) have been recognized and adopted for safety training in construction to enhance the safety performance of organizations. This study aimed to understand the state-of-art review of the DTs adopted for safety training and their application areas in construction since 2000. Fifty-nine articles were found after conducting a systematic literature review (SLR) using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) review method. The research trend was identified and described using bibliometric analysis in terms of the number of publications per year, sources of documents, influential authors and regions, highly cited articles, and research areas. The findings indicated that immersive virtual reality (VR) technology was used widely to train and educate individuals in construction research. In terms of research areas, most of the DTs were focused on enhancing individuals’ hazard recognition (HR) skills. The findings summarized the mainstream research areas, discussed existing research gaps, and suggested future directions in DTs application for safety training in construction. The recommended future directions could improve safety training performance in the construction industry.

Metasurfaces tuning is performed using different ways for wide range of applications. This study presents the design of thermally-tuned all-dielectric reconfigurable metasurface. A microfluidic channel, filled with different concentrations of tellurium – selenium (Te-Se) alloy, is added on the top of the elliptical dielectric resonator (EDR) unit cell of the considered metasurface. The electrical properties of used semiconductor alloy are varied in the range of 400°C to 700°C (steps size of 100°C). The impact of thermal tuning on the reflection and transmission characteristics of the designed metasurface is analyzed in the frequency range 20-30 GHz using COMSOL Multiphysics. Obtained results demonstrated that the realized metasurface exhibits reconfigurable behavior in terms of variations in the reflection and transmission characteristics with a change in either temperature or concentrations of selenium and tellurium. The wider bands with high reflection and low transmission frequency bands are obtained with lower concentrations of selenium and tellurium for all operating temperatures.