The brainstem locus coeruleus (LC) influences a broad range of brain processes and is suspected to be involved in many neurological disorders. The LC contrast is an accepted marker of the integrity and can be assessed with specific Magnetic Resonance Imaging (MRI) structural sequences. The small size of the LC has rendered its functional characterization difficult. A full characterization of the LC in healthy young and late middle-aged individuals is needed to determine to potential roles of the LC in different medical conditions. Here, we wanted to determine whether the activation of the LC in a mismatch negativity task changes in aging and whether the LC functional response was associated to the LC contrast. We used Ultra-High Field (UHF) 7-Tesla functional MRI (fMRI) during an auditory oddball task in 53 healthy volunteers, including 34 younger (age: 22.15y ± 3.27; 29 women) and 19 late middle-aged (age: 61.05y ± 5.3; 14 women) individuals. Whole-brain analyses confirmed brain responses in the typical regions previously associated with mismatch negativity. When focusing on the brainstem, we found a significant response in the rostral part of the LC (and most extensively in the left LC). Individual LC activity was not significantly different between young and late middle-aged individuals. Critically, while the LC contrast was higher in older individuals, the functional response of the LC was not associated with its contrast. These findings show that the age-related alterations of the LC structural integrity may not necessarily be related to changes in its functional response.

Introduction: The Kosovo War is widely remembered as one of the most brutal conflicts in the modern era. With over 13,000 deaths and 90% of the population displaced as refugees, the impact of the war has led to everlasting levels of trauma that impacts the lives of thousands of Kosovar-Albanians today. Objectives: To characterize the psychosocial impact of the Kosovo war by comprehensively assessing the literature to date. We also provide recommendations to improve outcomes for Kosovar-Albanian patients in the post-war era. Methods: A systematic review was conducted using post-war clinical psychological articles on the Kosovo War. Our search involved utilizing full-text English articles from PubMed, OVID Medline, and EMBASE journals. Keywords used were “Kosovo War”, “Psychosocial”, and “Post Traumatic Stress Disorder”. Results: Our post-traumatic stress disorder (PTSD) analysis yielded a total of 10,134 responses from participants ranging from refugees to the general public. Our results showed 2,997 subjects (30%) who reported or met the criteria for post-traumatic stress disorder. When analyzing refugee participants, there was 1,133 with 60% of them meeting the PTSD criteria. In a filtered cohort of 5,117 subjects measured for adverse events, 3,180 subjects (64%) of subjects had experienced combat, while 3,664 (74%) were exiled from their homes. Conclusion: The psychosocial impact of the Kosovo war is likely under-reported by patients due to cultural stigmas, language barriers, and trust barriers with clinicians. Factors such as age, employment, displacement status, number of traumatic events, socioeconomic vulnerability, and health literacy are associated with poor psychiatric morbidity.

Iron (Fe), manganese (Mn), copper (Cu) and zinc (Zn) are essential micronutrients that are necessary for plant growth and development, but can be toxic at supra-optimal levels. Plants have evolved a complex homeostasis network that includes uptake, transport, and storage of these metals. Previously, we reported that the OsbHLH156-OsIRO2 transcription factor (TF) complex is activated under Fe deficient condition and acts as a central regulator on Strategy II Fe acquisition. In this study, the role of the TF complex on Mn, Cu and Zn uptake was evaluated. Fe deficiency caused significant increased Mn, Cu and Zn concentrations in shoot. The increase of these divalent metal concentrations was significantly suppressed in osbhlh156 and osiro2 mutants, suggesting that the TF complex plays roles on Mn, Cu and Zn uptake and transport. RNA-sequencing assay showed that the genes associated with Mn, Cu, and Zn uptake and transport were significantly suppressed in the osbhlh156 and osiro2 mutants. Transcriptional activation assays demonstrated that the TF complex could directly bind to the promoters of OsIRT1, OsYSL15, OsNRAMP6, OsHMA2, OsCOPT1/7 and OsZIP5/9/10 and activate their expression. In addition, the TF complex is required to activate the expression of NA and 2’deoxymugineic acid (DMA) synthesis genes, which in turn facilitate the uptake and transport of Mn, Cu and Zn. Furthermore, OsbHLH156 and OsIRO2 promote Cu accumulation to partially restore the Fe-deficiency symptom. Taken together, the OsbHLH156-OsIRO2 TF complex functions as a core regulator not only in Fe homeostasis, but also in Mn, Cu and Zn accumulation.

Proteins play an essential role in the vital biological processes governing cellular functions. Most proteins function as members of macromolecular machines, with the network of interacting proteins revealing the molecular mechanisms driving the formation of these complexes. Profiling the physiology-driven remodeling of these interactions within different contexts constitutes a crucial component to achieving a comprehensive systems-level understanding of interactome dynamics. Here, we apply co-fractionation mass spectrometry and computational modeling to quantify and profile the interactions of ~2,000 proteins in the bacterium Escherichia coli cultured under ten distinct culture conditions. The resulting quantitative co-elution patterns revealed large-scale condition-dependent interaction remodeling among protein complexes involved in diverse biochemical pathways in response to the unique environmental challenges. Network-level analysis highlighted interactome-wide biophysical properties and structural patterns governing interaction remodeling. Our results provide evidence of the local and global plasticity of the E. coli interactome along with a rigorous generalizable framework to define protein interaction specificity. We provide an accompanying interactive web application to facilitate exploration of these rewired networks.

IntroductionGunshot wound injury is notorious for its variable pattern and projectile-related trauma, particularly in the spine. Generally, irrigation and debridement are accepted as the first-line operative treatments. However, it may not result in better outcomes in most low-energy gunshot wounds of the extremities [1]. Whether to remove the bullet immediately in patients with intact neurological function also remains a dilemma. While retaining the foreign body may lead to late migration and even neurological compromise [2], aggressive management may cause collateral iatrogenic injury [3]. Several studies have demonstrated a minimally invasive technique to remove bullets from the cervical spine [4,5]; however, similar procedures for the lumbar spine have rarely been mentioned. Advancements in navigation-assisted surgery have expanded the indications from thoracolumbar pedicle screws to deformity correction and spine tumor resection over the years [6]. Our aim is to examine the applicability of navigation-assisted surgery for lumbar spinal foreign body removal. In this case report, we successfully performed minimally invasive surgery to completely remove one bullet from the lumbar spine with minimal iatrogenic damage to the surrounding tissues by taking advantage of the accuracy and velocity supported by the navigation system.

Floodplain restoration can enhance capacity for carbon sequestration by facilitating higher water tables, deposition of fine sediment, and increased input and residence time of organic matter. We measured floodplain soil organic carbon stocks in nine stream restoration projects across the western United States and compared them to nearby degraded and reference condition floodplains. Degraded floodplains had the lowest soil mean carbon stocks in the majority of floodplains measured (range 161-894 Mg C/ha), and reference stocks had the highest stocks (range 391-904 Mg C/ha) of those with statistically significant differences between the three categories. Across all sites measured, stream restoration sites, referred to as treatment sites, had stocks (range 203-1028 Mg C/ha) similar to degraded condition floodplains but the largest range. When modeled under degraded conditions, four out of nine of the treatment sites had significantly higher OC stocks than predicted. Climate and geologic variables are most influential in predicting carbon stocks, and floodplains in the interior western USA have the highest carbon stocks. As the demand for carbon sequestration increases due to climate change, ecologically responsible floodplain restoration provides a significant opportunity for carbon storage. However, despite the statistically significant relationships we observed in this dataset, the variations within the data in relation to degraded/treatment/reference categories illustrate the uncertainties in quantifying the effects of restoration on floodplain carbon stocks.

A document by SIVABALAKRISHNAN R. Click on the document to view its contents.

High levels of airborne dust, frequent dust storms and infrequent rain events are some of the reasons why soiling can drastically reduce the energy yield of photovoltaic modules in desert areas. There are ongoing and increasing efforts to identify appropriate and economically feasible strategies that can be used to mitigate soiling in deserts. Both innovative tracking with adapted resting positions during night and anti-soiling coatings (ASC) are considered as potential solutions to reduce soiling. In this study, the individual mitigation potential of both ASC and tracking routines as well as the combination of the two approaches are investigated. For this, outdoor exposure tests were carried out in desert region of Saudi Arabia. Coated and uncoated glass samples were tested in different tilt configurations: fixed, 1-axis tracking with horizontal stowage (facing the sky) and 1-axis tracking with vertical stowage during the night. Both methods indicate significant soiling reductions, especially for the combined solution of ASC and tracking with vertical night stowage, where soiling losses can be reduced by up to 85%. In addition, it has been shown that by adapting tracking, the relative ASC performance can be improved compared to fixed tilt or standard 1-axis tracking scenarios.

Despite recent advances in prophylactic vaccination, SARS-CoV-2 infections continue to cause significant morbidity. A better understanding of immune response differences between vaccinated individuals with and without later SARS-CoV-2 breakthrough infection is urgently needed. CoV-ADAPT is a prospective long-term study comparing humoral (anti-spike-RBD-IgG, neutralization capacity, avidity) and cellular (spike-induced T-cell interferon-γ release) immune responses in individuals vaccinated against SARS-CoV-2 at four different time points (three before and one after third vaccination). In this cohort study, 62 fully vaccinated individuals presented with SARS‑CoV-2 breakthrough infections vs 151 without infection 3-7 months following third vaccination. Breakthrough infections significantly increased anti-spike-RBD-IgG (p<0.01), but not spike-directed T-cell interferon-γ release (TC), antibody neutralization capacity or avidity. Anti-spike-RBD-IgG and antibody avidity decreased with age (p<0.01) and females showed higher anti-spike-RBD-IgG (p<0.01), and a tendency towards higher antibody avidity (p=0.051). The association between humoral and cellular immune responses previously reported at various time points was lost in subjects after breakthrough infections (p=0.807). Finally, a machine-learning approach based on our large immunological data set (a total of 49 variables) from different time points was unable to predict breakthrough infections (AUC: 0.55). In conclusion, distinct differences in humoral vs cellular immune responses in fully vaccinated individuals with or without breakthrough infection could be demonstrated. Breakthrough infections predominantly drive the humoral response without boosting the cellular component. Breakthrough infections could not be predicted based on immunological data, which indicates a superior role of environmental factors (e.g. virus exposure) in individualized risk assessment.

Integrating chatbots (ChatGPT) in the process of manuscript writing and proposing a roadmap for their future adoptionMuhammad Ali1,*, Nazir Ahmad2, Hussein A Younus31 Department of Life Sciences, School of Science, University of Management and Technology (UMT), Johar Town, Lahore 54770, Pakistan. muhammad_ali@umt.edu.pk; ali4982@gmail.com2 Department of Chemistry, Government College University Lahore, 54000, Pakistan. dr.nazirahmad@gcu.edu.pk3 Nanotechnology Research Centre, Sultan Qaboos University, PO Box 17, PC 123, Al-Khoud,Oman. hay00@fayoum.edu.egArtificial intelligence (AI) language chatbots overwhelmed the scientific community these days, specifically the ChatGPT1 . From writing assignments to the original manuscripts, it influenced to an extent that it was listed as one of the authors at few instances2,3raising debate among the scientific community. Before AI language bots, language editing tools were commonly used by the scientific community, however, chatbots are considered superior in context with the speed, refined language, and the so-called contribution. However, there are some serious concerns of the global scientific community regarding the chatbots’ judicial use.Although ChatGPT is a promising tool assisting scientists encompassing relevant literature / data and writing manuscripts in an impressive way, it is not without the associated threats, like generation oferroneous citations.4 Also, the output may be manipulated supporting arguments with tailored efforts, the text-output generated, therefore, needs critical review and consultation(s) / verification(s) with the original literature.5Looking into the recent updates, and to allow its judicial use, the following measures may be taken: (a ) authorship for the language chatbots should be discouraged as these tools cannot be held accountable for any statement or any ethical breach, (b ) it would, in similar way, not appropriate to acknowledge the tool in the ‘acknowledgment section’ as the more appropriate was would be to cite it, instead, and (c ) the most importantly, there are tools developed to detect text generated with language chatbots, at least GPTZero6 . And that the experience with ChatGPT has raised concerns over both the health of the generated content and citations, there is a dire need to re-enforce measures. One way to deal with it is to get disclaimer or the more appropriate would be to have a cutoff value of using ChatGPT in a manuscript. Just like a cutoff for similarity index/plagiarism (i.e. , 19%), for instance. Similar cutoff for the judicial use language chatbots be devised. This may, on one hand, allow its judicial use and, on the other side, will allow the reader the extent to which the manuscript under study be relied upon.

For diagnosis of CMV infection in colon biopsies, immunohistochemistry (IHC) and polymerase chain reaction (PCR) can be helpful besides pathologic examination. We performed CMV IHC and PCR on formalin–fixed paraffin-embedded (FFPE) and fresh tissue (FT) in clinically suspicious children for colitis and compared the results with the patients’ clinical course. In a prospective cohort study, among 153 candidate children for the colonoscopy due to colitis, 50 showed positive CMV PCR on FT. PCR on FFPE tissue was positive in 17/40 (42.5 %) cases, of which 2/40 (5%) had positive IHC. Low titer plasma CMV DNA level was seen in 7/17 FFPE-PCR positive cases. Two patients had a high plasma CMV DNA level, who were the two IHC-positive cases. Fecal calprotectin level showed an association with disease histologic activity, but not with CMV positivity on either of the methods of detection. IHC positivity was more likely to be associated with a high plasma CMV DNA level. There was a high rate of false positivity by CMV PCR test on fresh tissue that was neither associated with histopathology nor with the CMV DNA plasma level. Antiviral treatment was correlated with a poorer outcome in patients with inflammatory bowel disease (P=0.035). Conclusion: The PCR result of FT is correlated neither to histology nor to the serum CMV-DNA level. High fecal calprotectin level is associated with active lesions in histopathology. Large randomized clinical trials are recommended to assess the efficacy of antiviral treatment in acute colitis cases that have a high copy number of CMV in the blood or positive results on IHC.

A document by Niranjan Thapa. Click on the document to view its contents.

A targeted sequencing technique was applied to detect cases of suspected congenital thrombocytopenia as a case series study. Six of 18 patients tested had positive results from the existing catalogue, while a definitive diagnosis was not determined for the remaining 12. Two neonates revealed defects in PTPN11, indicating Noonan syndrome.

Mesozooplankton is a key component of the ocean, regulating global processes such as the carbon pump, and ensuring energy transfer from lower to higher trophic levels. Yet, despite the importance of understanding mesozooplankton diversity, distribution and connectivity at global scale to predict the impact of climate change in marine ecosystems, there is still fragmented knowledge. To fill this gap, we applied DNA metabarcoding to mesozooplankton samples collected during the Malaspina-2010 circumnavigation expedition across temperate and tropical oceans from the surface to bathypelagic depths. By conducting a hidden diversity analysis, we highlight the still scarce knowledge on global mesozooplankton diversity and identify the Indian Ocean and the deep sea as the most understudied areas. By analysing mesozooplankton community spatial distribution, we confirm global biogeographical patterns across the temperate to tropical oceans both in the vertical and horizontal gradients. Additionally, we reveal a consistent increase in mesozooplankton beta-diversity with depth, indicating reduced connectivity at deeper layers, and identify a water mass type-mediated structuring of bathypelagic communities, instead of an oceanic basin-mediated as observed at upper layers. This suggests limited dispersal at deep ocean layers, most likely due to weaker currents and lower mixing of water mass types. Overall, our work supports the neutral theory of biodiversity and thus the importance of oceanic currents and barriers in dispersal in shaping global plankton communities, and provides key knowledge for predicting the impact of climate change in the deep-sea.

Dry ice is one of the world’s most in-demand commodities for cold chain distribution of temperature sensitive products in insulated shippers. A rule of thumb for packaging dry ice has been that it sublimates about 2.3 to 4.5 kg every 24 hours, without any consideration for specific dry ice type, size or geometry [(CISA, 2020)](#ref-0004). This study explored sublimation rates of dry ice composition (binding agent and density), age, and geometry (size and shape). Dry ice block manufacturing often involves dosing propylene glycol-water “binder” solution to liquid carbon dioxide. Tests performed in this study revealed that the binding agent did not influence the dry ice sublimation rate. Dry ice density was found to impact sublimation rate. Fresh and aged blocks of similar dimensions and weight were found to have similar performance outcomes. Weight loss curves for pellets and various sized dry ice blocks showed a dependence on surface area and volume. Chunked blocks and pellets with and without binding agent sublimated at similar rates of 2.84 % h -1 and 2.53 % h -1 (w/w), respectively, whereas block varieties with significantly lower surface area to volume ratios had lower sublimation rates of 0.98-1.60 % h -1.

1. IntroductionChikungunya fever is a persistent joint pain disease caused by mosquito-borne arboviruses: chikungunya virus (CHIKV). In Chikungunya fever, inflammatory cytokines such as IL-6, Il-17, IL-22, and IL-23 ［1,2］ are involved in chronic joint pain, but there are no reports on whether IL-36 is specified as a cause of joint pain. This case study compares the changes in IL-36α and β levels during infected chikungunya fever and the recovery. This article highlights the relationship between chikungunya fever and IL-36.

Background: Several recent studies have investigated the association between maternal diet during pregnancy and asthma in children. However, whether a specific dietary pattern during pregnancy protects children from asthma remains unclear. This study investigated the association between a healthy diet, dietary inflammation potential during pregnancy, and the risk for wheezing and atopic eczema in children. Methods: This study included 1330 mother-child pairs who attended the Kuopio Birth Cohort (KuBiCo) study and had dietary information during the last trimester and information on children’s health in the first year of life. The Alternative Healthy Eating Index for Pregnancy (AHEI-P) and Dietary Inflammatory Index (DII) indicate a healthy diet and dietary inflammation potential during pregnancy. The AHEI-P and DII were compared with reported wheezing and doctor-diagnosed atopic eczema in children during the first year of life. Results: Neither the AHEI-P nor the DII was associated with wheezing or atopic eczema in children when analyzed by continuous variables or tertiles. The odds ratio for AHEI-P and wheezing was 0.99 (0.98–1.01); for AHEI-P and atopic eczema was 1.01 (0.99–1.02); for DII and wheezing was 1.02 (0.95–1.09), and for DII and atopic eczema was 0.97 (0.91–1.04). Conclusion: In this cohort study, a maternal healthy or anti-inflammatory diet during pregnancy was not associated with wheezing or atopic eczema in the first year of life. Interventions in the overall dietary pattern during pregnancy might not effectively prevent atopic diseases in children.

Background: Asthma is a major health issue with an extensive burden to family and health systems. The purpose of this study was to assess the burden of illness among school children with asthma in Jordan. Methods: A descriptive exploratory design was used. Children with asthma (7-14 years of age) and their parents completed questionnaires about asthma management and burden including symptoms and health services use. An assessment of children’s proper use of inhalers was conducted. Results: Outcomes indicate high burden of illness manifested in the majority of children who experienced symptoms, asthma attacks, ER visits, and admission because of their asthma. The majority performed incorrect inhaler technique which reflects uncontrolled asthma and poor management. Parental perception of their child’s health compared well with their burden of asthma. Conclusions: The high reported burden (symptoms and services use, use of inhalers) calls for collaboration between professionals and stakeholders with children and caregivers in planning and implementing care. This study supports the importance of parental perceptions in managing their child’s asthma.

Using manure appropriately can enhance organic carbon, and hydro-physical properties without negatively impacting the environment. However, how manure impacts soils, especially at deeper depths, is still not well studied. Therefore, the objective of this study was to assess the impact of different manure and inorganic fertilizer application rates on soil profile organic carbon and hydro-physical properties under corn(Zea mays L.)-soybean(Glycine max L.)-spring wheat (Triticum aestivum L) rotation at Beresford (initiated, 2003) and Brookings (2008) sites in South Dakota. Treatments included: low manure(LM), medium manure(MM), high manure(HM), medium fertilizer(MF), high fertilizer(HF), and control(CK). Four replicated intact soil cores were collected from all the treatments at 0-10, 10-20, 20-30, and 30-40 cm depths. Considering treatments by depth interactions, the HM increased the SOC and TN stocks by 16-62% as compared to the CK, MF, and HF at 0-10 cm for either site. However, treatments did not always impact these parameters beyond 20 cm depth. On observing treatment as the main effect, the MM and HM increased the SWR at 0, -5 kPa compared to MF, HF, and CK in Brookings, and the MM increased the SWR at -30 kPa as compared to the MF in Beresford at 0-40 cm depths. Data suggests continuous manure application may enhance organic carbon and hydro-physical properties at deeper depths. Therefore, this study concluded that long-term manure application is more beneficial when you consider the application rate and its timing. It can improve hydro-physical properties, thereby stabilizing the soil structure and improving water retention at deeper depths.

Disturbance and connectivity control biodiversity, ecosystem functioning and their interactions across connected aquatic and terrestrial ecosystems, that form a meta-ecosystem. In rivers, detrital organic matter (OM) is transported across terrestrial-aquatic boundaries and along the river network and decomposed on the way by diverse communities of organisms, including microorganisms and invertebrates. Drying naturally fragments most river networks and thereby modify organism dispersal and OM transfers across ecosystems. This may prevent organisms from reaching and consuming OM, generating mismatches between community composition and decomposition. However, little evidence of the effects of drying on river network-scale OM cycling exists. Here, we aim to examine the effects of fragmentation by drying on the structure of consumer communities and ecosystem functioning within interacting aquatic-terrestrial river ecosystems. We monitored leaf resource stocks, invertebrate communities and decomposition rates in the instream and riparian habitats of 20 sites in a river network naturally fragmented by drying. Although instream resource quantity and quality increased with drying severity, decomposition decreased due to changes in invertebrate communities and particularly leaf-decomposer abundance. Invertebrate-driven decomposition peaked at intermediate levels of upstream connectivity, suggesting that intermediate levels of fragmentation can promote the functioning of downstream ecosystems. We found that the variability in community composition was unrelated to variability in decomposition at sites with low connectivity and high drying severity, suggesting that such conditions can promote mismatches between community composition and decomposition. Decomposition instream was correlated to decomposition in the riparian area, revealing one of the first network-scale evidence of the links between ecosystem functions across terrestrial-aquatic boundaries. Our river network-scale study thus demonstrates the paramount effect of drying on the dynamics of resources, communities and ecosystem functioning in river networks, with crucial implications for the adaptive management of river networks and preservation of their functional integrity.

Aflatoxin and other mycotoxin contamination are major threats to global food security and present an urgent need to secure the global food crop against spoilage by mycotoxigenic fungi. Cocoa material is noted for naturally low aflatoxin contamination. This study was designed to assess the potential for harnessing cocoa-associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus. The candidate fungi were isolated from fermented cocoa beans collected from four cocoa-growing areas in Ghana. Molecular characterization included ITS-sequencing for identification and PCR to determine mating type. Effects of the candidate isolates on growth and aflatoxin-production by an aflatoxigenic A. flavus isolate (BANGA1) were assessed. Aflatoxin production was monitored by UV fluorescence and quantified by ELISA. Thirty-six filamentous fungi were cultured and identified as Aspergillus, Cladosporium, Lichtheimia or Trichoderma spp. isolates. The isolates generally interacted negatively with BANGA1 growth and aflatoxin production. The A. niger and A. aculeatus biocontrol candidates showed the strongest colony antagonism (54–94%) and reduction in aflatoxin production (12–50%) on agar. In broth, the A. niger isolates reduced aflatoxin production by up to 97%. Metabolites from the A. niger isolates showed the strongest inhibition of growth by BANGA1 and inhibited aflatoxin production. Four of the candidate isolates belonged to the MAT1-1 mating type and 12 identified as MAT1-2. This may be indicative of the potential for genetic recombination events between fungi in the field, and finding which is particularly relevant to the risk posed by A. flavus biocontrol measures that rely on atoxigenic A. flavus strains.

Many attempts have been made in the past to regain the spectral accuracy of the spectral methods, which is lost drastically due to the presence of discontinuity. In this article, an attempt has been made to show that mollification using Legendre and Chebyshev polynomial based kernels improves the convergence rate of the Fourier spectral method. Numerical illustrations are provided with examples involving one or more discontinuities and compared with the existing Dirichlet kernel mollifier. Dependence of the efficiency of the polynomial mollifiers on the parameter P is analogous to that in the Dirichlet mollifier, which is detailed by analysing the numerical solution. Further, they are extended to linear scalar conservation law problems.

Although IE incidence has increased due to the gradual increase of survival rate in congenital heart disease (CHD) children, the mitral and aortic valves are the most commonly involved, while tricuspid valve infection is rare, and pulmonary valve involvement is extremely rare. To improve the diagnosis in echocardiography, we described two cases of right-sided infective endocarditis (RSIE), explained the ideas of ultrasonic diagnosis, analyzed the differential diagnosis, and demonstrated the important role of echocardiography in diagnosing and treating children.

According to the dynamic, complex, and opening characteristics of land ecosystems, we constructed an evaluation indicator system for early-warning of land ecosystem health (LEH) based on a perspective of state–danger–response. Then, we analyzed the warning condition of LEH in the Poyang lake eco-economic zone in China over the past decade using the variable weight matter-element model. The LEH status in the study region improved obviously from 2010 to 2020. The warning condition of regional LEH in 2010, 2015, and 2020 was in the “warning”, “relatively healthy”, and “healthy” stages, respectively. The evolution of LEH was better demonstrated by the variable weight matter-element model than by the traditional method because the former could modify the weight of early-warning indicators and eliminate the incompatibility between indicators. Our study provides an alternative approach for research into ecosystem health, and the results will be useful for improving LEH in the study region.