Background & Purpose: Reactive oxygen species (ROS) play pivotal roles in cellular signaling. Nrf2 is a key transcription factor that regulates redox homeostasis. However, the relationship between Nrf2 and H2O2 is controversially discussed in the literature. Thus, we aimed to investigate how varying intracellular H2O2 levels, manipulated through different methods, influence Nrf2 regulation in brain endothelial cells under physiological normoxia (5 kPa O₂), contrasting with typical hyperoxic cell culture conditions. Experimental Approach: In this study, we developed and validated a novel oxygen-independent Nrf2 biosensor, Pericellular Oxygen-Insensitive Nrf2 Total LEvel Reporter (POINTER), using human cerebral microvascular endothelial cells (hCMEC/D3). We investigated the relationship between intracellular H2O2 and Nrf2 levels under varying oxygen conditions, employing exogenous H₂O₂ application, chemogenetic production of H2O2 via modified D-amino acid oxidase (mDAAO), and pharmacological induction of H2O2 with Auranofin. Key Results: POINTER confirmed significantly lower Nrf2 levels in hCMEC/D3 cells under physiological normoxia (5 kPa) compared to hyperoxia (room air). Auranofin demonstrated efficacy in modulating intracellular H2O2 increase comparable to exogenous administration of H₂O₂, yet H₂O₂ levels recovered to the baseline 24 hours after this treatment. Moreover, only chemogenetically produced H2O2 remained significantly elevated in cells after 24 hours. More importantly, only Auranofin, but not exogenous provision of H2O2 and chemogenetically induced oxidative stress robustly increased Nrf2 levels under physiological oxygen conditions demonstrated by the POINTER biosensor. Conclusion and Implications: These findings reveal the intricate regulation of Nrf2, indicating that both antioxidant inhibition and the elevation of H₂O₂ are crucial for modulating Nrf2 levels under physioxia.

In this paper, we raise five questions about the Internet, which are: What are the positive and negative effects of broadband Internet? Which type of Internet access is preferable? Which one of them needs more attention? How much and what kind of developments affect the Internet? What solutions are there to improve the current situation? and find solutions for them. First, fourteen advantages and positive effects of broadband Internet in every corner of life are presented. Second, fifty criteria are extracted for comparing wired and wireless broadband Internet. The third task, they are compared by describing the characteristics of optical fiber-based fixed and fifth-generation-based fixed/mobile broadband Internet access. Fourth, issues that directly and/or indirectly affect the global development of broadband Internet are outlined. The fifth task elicits twenty items on balanced sustainable development and strategic planning of broadband Internet. Finally, we propose ten solutions to improve the current state of global broadband Internet, reduce the digital divide in the world, and decrease the negative social effects of broadband Internet.

A document by Joseph Julius. Click on the document to view its contents.

The aperiodic slope of the EEG power spectrum (characterized by a 1/fx distribution in which power decreases as frequency increases) is hypothesized to index the excitatory-inhibitory balance and has been associated with various neurodevelopmental outcomes in older children and adults. However, the dearth of research early in development led us to investigate associations among EEG aperiodic slope, temperament, and maternal internalizing (anxiety and depression) symptoms in a large cohort of typically developing infants. Steeper slope was associated with higher scores on the temperament domains of orienting/regulation and surgency but was not associated with negative affectivity. Maternal symptoms did not appear to be directly associated with slope, but slope moderated the association between maternal symptoms and temperament. Specifically, steeper slope was associated with a stronger negative association between maternal internalizing symptoms and infant orienting/regulation. These results demonstrate associations between slope and behavior as early as infancy, which may reflect early differences in the development of global inhibitory networks. Longitudinal research in early childhood is necessary to better understand the nature of these relations during development and their potential impact on later socioemotional outcomes.

Purpose. To assess relative efficacy of dapagliflozin and empagliflozin in routinely treated chronic heart failure (CHF) patients. Methods. In this single-center registry analysis, prevalent and incident CHF patients with a wide range of left ventricular ejection fraction values started on dapagliflozin or empagliflozin in addition to other guideline-directed therapy were mutually balanced on a range of characteristics, and were assessed for incidence of a composite of all-cause death/major adverse cardiac events (primary outcome) over the initial 6 months of treatment, and for New Your Heart Association (NYHA) functional class at 6 months (secondary outcome). Frequentist and Bayes (with a moderately informed skeptical prior) estimates were generated for dapagliflozin vs. empagliflozin comparison. Results. In both prevalent (dapagliflozin n=393, empagliflozin n=328) and incident (dapagliflozin n=124, empagliflozin n=116) patients, those prescribed dapagliflozin had somewhat higher incidence of the primary outcome and were more likely to present with a worse NYHA class at 6 months, but the estimates were imprecise. In the pooled data, primary events (102 in total) were more common in dapagliflozin-prescribed patients (frequentist estimate RR=1.519, 95%CI 1.239-1.861; Bayes RR=1.380, 95%CrI 0.981-1.944). Dapagliflozin-prescribed patients were also were more likely to have a worse NYHA class at 6 months (OR=1.540, 95%CI 1.208-1.962; Bayes OR=1.425, 95%CrI 1.098-1.781). Conclusion. CHF patients prescribed with dapagliflozin apparently had poorer outcomes than those prescribed with empagliflozin over the initial 6 months of treatment. Data emphasize a need for a direct randomized comparison of the two treatments in this setting.

Tongue squamous cell carcinoma and oral lichen planus: a rare case report of a pregnant womanDavid Moretti1,2, Joël Cucherousset3, Nadia Benlagha4 and Ihsène Taïhi1,5,6(1) Department of Dentistry, Faculty of Dental Médicine, Université Paris Cité, 1 Rue Maurice Arnoux, 92120 Montrouge, France(2) Department of Oral Surgery, La Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris, 47-83 Boulevard de l’Hôpital, 75013 Paris, France(3) Department of Pathology, Le Raincy-Montfermeil Intercommunal Hospital Group, 10 Rue du Général Leclerc, 93370 Montfermeil, France(4) Department of Oral Surgery, Sud Francilien Hospital, 40 avenue Serge Dassault 91100 Corbeil-Essonnes(5) Department of Oral Surgery, Rothschild Hospital, Assistance Publique-Hôpitaux de Paris, 5 Rue Santerre, 75012 Paris, France(6) Orofacial Pathologies, Imaging, and Biotherapies Laboratory (URP 2496 BRIO), Université Paris Cité, 1 Rue Maurice Arnoux, 92120 Montrouge, FranceCorresponding author : davidmoretti95@gmail.com

Magnetic resonance imaging (MRI) has significantly improved the monitoring of tumor ablation by providing high-contrast soft tissue images. However, MRI-guided surgical procedures often involve extremely delicate and complex manipulations, requiring an MRI-compatible robotic system to enhance precision and reduce human error from jitter during prolonged high-precision tasks. This article introduces a novel MRI-compatible, hydraulically actuated needle insertion robot for minimally invasive stereotactic neurosurgery. The robot operates based on the principles of spherical chain mechanisms and communicating vessels, being driven by a linear piston and actuator. A double-layer spherical chain mechanism translates the linear motion of the piston into rotational motion. An MRI-compatible linear encoder is constructed using a grating ruler and optical fiber to ensure precise control. Model predictive control (MPC) enabled the robot to track a given trajectory accurately, allowing for precise braking pressure management and fast response times. Testing and analysis revealed that the robotic system achieves an average positional accuracy of 1.435 mm, with angle accuracies of 0.401°. Additionally, the precision and repeatability of the robot fall within the ideal range. The results demonstrate that the developed puncture robot is both materially and structurally compatible with the MRI environment, showcasing effective spatial positioning and trajectory planning capabilities.

The detection of defects on the copper chain in the production process of tobacco cutters is crucial for ensuring product quality. Traditional defect detection methods often rely on spatial domain image analysis, which not only has a large computational load but also performs poorly in handling high-frequency noise and complex backgrounds. To address this issue, this paper proposes a novel neural network model based on frequency domain analysis, called Frequency Domain Attention Network (FDANet). This network first utilizes Discrete Cosine Transform (DCT) to transform the image from the spatial domain to the frequency domain, effectively reducing computational complexity and improving processing speed. Subsequently, through the innovative Frequency Domain Attention Module (FDAM), the network automatically identifies and enhances key discriminative features in the frequency domain, thereby strengthening the model’s ability to identify defects. Finally, the frequency domain attention map, after feature extraction and integration, is inputted into the coupling detection head to achieve high-precision defect detection. Experimental results demonstrate that FDANet performs excellently in the task of detecting copper chain defects in tobacco cutters, showing significant improvement compared to traditional methods and verifying the effectiveness and practicality of the proposed approach.

Objective: Validate diagnostic accuracy of new unique biomarker, gastrointestinal myoelectrical activity (GIMA), detected by electroviscerography (EVG) with Ai-derived disease threshold score calculation to noninvasively diagnose endometriosis. Design: Multicenter prospective blinded trial Setting: Women’s Healthcare Center Population of Sample: 165 patients with and without endometriosis diagnosis Methods: Initial 50 patients meeting inclusion criteria in 165-patient multicenter prospective GIMA biomarker trial were selected for interim analysis. Study population included women 27-55 years old, 25 with diagnosis of endometriosis and 25 non-endometriosis controls. Clinical and GIMA data were collected between February 2007 and September 2017, at all harvesting time points and frequency bands using EVG. Ai-derived threshold score calculations used area under the curve (AUC), age and standardized pain scores variables. Main Outcome Measures: Specificity, sensitivity, NPV, PPV and predictive probability or C-Statistic from logistical regression analyses of all AUC frequency and time points. Results: Non-endometriosis versus endometriosis cohort interim analysis differed significantly (p<0.001) for median (IQR), AUC values, and percent frequency power distribution at baseline, 10-minute, 20-minute, and 30-minute post water-load at frequency ranges 15-20cpm, 30-40cpm, 40-50cpm and 50-60cpm. GIMA threshold scoring revealed sensitivity and PPV of 96%, specificity and NPV of 96% and C-statistic of 100%. Ai-derived GIMA biomarkers threshold scoring predicted 25/25 subjects positive and negative for endometriosis, with surgical confirmation. Hormonal therapy, surgical stage, age nor pain score affected diagnostic accuracy. Conclusions: EVG GIMA biomarker data with Ai-derived threshold scoring accurately distinguished participants with and without endometriosis. This interim analysis supports continued investigation of GIMA biomarkers to diagnose endometriosis.

A document by Wanlin Liu. Click on the document to view its contents.

Cholecystocolonic fistula: A rare presentation of colon adenocarcinomaMojtaba Niknami 1*, Amirhosein Kabiri 1, Rasoul Ebrahimi 1, Iman Razeghian 11 School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran*Correspondence to: Dr. Mojtaba Niknami, Assisstant professor of general surgery, Shahid Beheshti University of Medical Sciences, Tehran, IranE-mail: dr.niknami2019@gmail.com

Rice blast, caused by the destructive fungal pathogen Magnaporthe oryzae, poses a significant threat to global rice production. Developing resistant rice varieties using new resistant sources is the most effective method to manage this disease. This study aimed to (i) evaluate the phenotypic responses of forty-four native rice germplasms from Bangladesh to M. oryzae, and (ii) identify major blast resistance genes present in these germplasms using molecular markers. Among the evaluated germplasms, nine (Acc. 3058, Acc. 3060, Acc. 3068, Acc. 3071, Acc. 3073, Acc. 3975, Acc. 3076, Acc. 3083, and Acc. 3085) exhibited moderate resistance, while one (Acc. 3080) displayed very high resistance to M. oryzae. Out of eight molecular markers tested, the marker Pita3, which is tightly linked with the Pita gene, showed a significant association (p=0.003, R2=0.19) with blast resistance. Based on the combined genotypic and phenotypic data, the rice genotypes were grouped into two distinct population subgroups, with Acc. 3076 forming a separate cluster in the analysis. Our findings suggest that Acc. 3058, Acc. 3075, Acc. 3076, and Acc. 3080 are promising genetic resources for developing durable blast-resistant rice varieties and for the discovery of new resistance genes against M. oryzae.

Key Clinical Message:This Letter to the Editor comments on and aims to clarify details regarding the case, 26 cm fall caught on video causing subdural hemorrhages and extensive retinal hemorrhages in an 8-month-old infant . The findings in this case are rarely reported in accidental injury events and are therefore, important to understand.We read with interest the case report by Brook et al. of an 8-month-old with subdural and retinal hemorrhages following a fall from a couch with inertial rotational forces and possible previous head injury1. We thank the authors for the details provided, which are important in considering the differential diagnosis in a particular case, including the possibilities of accidental and inflicted head trauma, and any possible contributing factors. The existing literature, including systematic reviews from Canada, Australia and the UK supports the predominant opinion that subdural hemorrhages and retinal hemorrhages are highly associated with inflicted injury2-6. As a result, this case report is expected to be of interest and importance to others as they assess cases with findings which may raise concern for inflicted injury. We therefore are requesting further details regarding this case.The authors appropriately note previous falls and the possibility that these contributed to the symptoms and findings. The pattern of head circumference increase can be helpful clinically in understanding the sequence of events and underlying contributors to bleeding. The authors noted that the head circumference was large at the 99th percentile one month prior to presentation to hospital. It was reported that one month prior to presentation, a fall from a trampoline occurred and there was an episode of shoulder and arm twitching. Could the authors clarify the timing of the fall from the trampoline, relative to the timing of the twitching symptoms and the date of the head circumference? Additionally, is the previous pattern of head circumference growth known to the authors?The authors describe symptoms of vomiting and poor eating two days prior to the fall from the couch. We note that these can be symptoms of traumatic head injury, in addition to other possible etiologies. Could the authors clarify the clinical assessment regarding the cause of these symptoms?Abnormalities of coagulation are typically considered in the differential diagnosis of infants presenting with bleeding (subdural hemorrhages and retinal hemorrhages). Could the authors clarify whether a bleeding disorder work-up was completed and if so, it would be helpful to understand which tests were completed and the results?We are interested in the details of the head imaging. Were the subdural hemorrhages all supratentorial, or were there also infratentorial hemorrhages? Would it be possible to share an axial or coronal MRI FLAIR image, which may provide improved visualization of the subdural and subarachnoid spaces?We note the ophthalmology comment that the optic nerves in each eye were mildly swollen, suggesting possible increase in intracranial pressure. Was there indication of cerebral edema on the CT scan completed on the day of the described event? It would also be helpful to understand the clinical course of symptoms following the day of the fall from the couch and during hospitalization.Case reports of this nature are critical to deepening our understanding of potential mechanisms for subdural hemorrhages and retinal hemorrhages in infants. We read this case report with interest and appreciate the authors’ efforts in responding to our questions.

HIV exhibits significant genetic diversity, with genetic recombination being a major evolutionary process. The co-circulation of HIV-1/M and HIV-1/O variants has led to the description of 20 HIV-1/M+O dual infections since 1998. Despite the genetic divergence between these variants, HIV-1/M+O dual infections have resulted in the emergence of HIV-1/MO intergroup recombinant forms, with 20 unique HIV-1/MO recombinant forms (URF_MO) currently described, raising the question of a possible benefit of the recombination and the modalities of their emergence.. This review summarized the current knowledge on HIV-1/MO recombinant forms, including their virological and genetic characteristics, phylogenetic analysis, genome profiles, and breakpoints number and location. This work also identified the potential impacts of HIV-1/MO recombination on diagnosis, monitoring, and treatment, as well as the replicative capacity of such recombinants. This review highlighted the greater diversity and complexity of HIV-1/MO recombinants than originally thought, offering new research perspectives on their emergence and virological properties.

Aims Conventional management (CM), substantial fertilization (furrow and hole application) and flooding irrigation, has led to soil acidification, the decrease of soil bacterial diversity in bamboo forests. Integration of water and fertilizer management (IWF) can effectively improve the utilization efficiency of water and fertilizer, but its effect on soil environment, especially on microbial communit, is still unclear. Methods Here, we used next-generation high-throughput sequencing to compare soil properties and bacterial communities through different fertilization and irrigation methods under IWF and CM. Results Compared to the control group, CM significantly reduced soil pH and bacterial diversity, while IWF increased soil pH, improved soil nutrition status, and increased soil bacterial diversity to a level similar to the control group. Compared with CM, IWF also improved the relative abundance of beneficial bacteria and copiotrophic bacteria community in the soil, and the bacterial community in IWF clustered well with the control group. The structure of the bacterial community was also significantly correlated with soil total nitrogen, hydrolyzable nitrogen, available potassium and soil organic matter, while soil bacterial diversity was mainly associated with soil hydrolyzable nitrogen. Conclusions IWF can play an important role in preventing soil acidification, the loss of soil bacterial diversity, and improving the structure of the bacterial community.

The introduction of self-sampling in cervical cancer screening has raised the importance of HPV testvalidation on self-collected samples. This study aimed to evaluate the clinical performance of the OncoPredict HPV Screening (SCR) assay on self-collected vaginal and first-void urine (FVU) as part of the VALHUDES framework. Vaginal (FLOQSwabs) and FVU (Colli-Pee) samples were self-collected by 500 women referred to colposcopy, followed by a clinician-collected cervical sample prior to colposcopy, which were all tested using OncoPredict HPV SCR. OncoPredict HPV SCR demonstrated similar relative clinical sensitivity to detect cervical intraepithelial neoplasia grade 2 or worse (≥ CIN2) in urine (ratio: 0.95 [95%CI 0.88-1.02]) and vaginal self-samples (ratio: 0.96 [95%CI 0.90-1.02]) compared to cervical samples. The clinical specificity was lower in vaginal but not in urine samples compared to cervical which improved following cut-off optimization. A higher cellularity was found in vaginal as compared to cervical and FVU samples. Moderate to excellent agreement in HPV detection in self-collected samples and cervical scrapes was demonstrated (Kappa values: 0.53 to 1.00). OncoPredict HPV SCR assay demonstrated similar accuracy on self-collected vaginal and FVU samples compared to cervical samples, although cut-off adjustment improved clinical specificity when applied to vaginal samples.

Intrathecal Pain Pump Causing Delayed Acute Flaccid Paralysis: A Case Report and Review of the LiteratureMegan Finneran, DO, MS1*; Emilio Nardone, MD1Affiliations:1Department of Neurosurgery, Carle BroMenn Medical Center, Normal, IL, USA

Case report: severe mosquito bite allergy as a symptom of relapsed adult chronic active Epstein-Barr virus disease and its poor prognosisLian Liu1,2†, Sida Peng1,3†, Zhanwu Lv4, Danyun Yuan1,2, Boyun Shi1,2, Chenrui Zhuo1,2, Wenhui Gu1,2, Zhenqian Huang1,3 *and Huo Tan1,2 *1 Guangzhou Medical University, Guangzhou, 511436, China2 Department of Hematology, Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou,510700, China3 Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou,510120, China4 Guangzhou Jinyu Medical Testing Center Co., Ltd, Guangzhou,510005, ChinaLian Liu1,2† and Sida Peng1,3†These authors contributed equally to this work and share first authorship

Early approval mechanisms, such as conditional approval in the EU, have been used extensively to provide timely access to therapeutic innovations to cancer patients with unmet medical needs. While based on promising early evidence, such approvals are challenging from many perspectives due to the lack of comprehensive data. The limitation typically relates to data that demonstrates clinical benefit via particular endpoints and is only acceptable when the early evidence is particularly convincing to assume that the benefits of early access are greater than the potential harms. This paper describes the requirements for conditional approval and reviews common pitfalls in oncology, such as misunderstandings about the strength of evidence from exploratory trials and secondary analyses, lack of planning, and opportunities to improve communication. Thereafter, we present a framework (“EDGE”) on how to improve the submission and evaluation of drug applications for conditional approval in the EU.

Microstates are periods of quasi-stability in large-scale neural networks, and they are ubiquitous spanning sleep, rest and behaviour. One possibility is that the temporal and spatial features of these states vary from person to person contributing to inter-individual behavioural differences. Another possibility is that the microstates support momentary behavioural needs and as a result their properties may vary with behavioural fluctuations in the same person. Here, we leverage the history of smartphone touchscreen interaction dynamics and combined smartphone and EEG recordings (N = 61), to address if and how microstates contribute to real-world behaviour. We find that microstates measured when at rest correlated with the history (~30 days) of smartphone interaction dynamics captured in the real world, indicating their role in the behaviour. During smartphone behaviour, the configuration of microstates varied over time. However, this variance was largely unrelated to the rich behavioural dynamics, as in similar temporal and spatial features were observed during rapid and slow smartphone interactions. We show that in the real world microstates do not respond to the fluctuations of the ongoing behaviour. Still, as microstates measured at rest are correlated to the inter-individual behavioural differences captured on the smartphone, we propose that microstates exert a top-down influence to broadly orchestrate real-world behaviour.

Aims: Epidermal necrolysis (EN) is a rare and severe condition characterized by a diffuse skin and mucosal detachment, mainly induced by drugs. Literature is scarce regarding the rate of recurrences and drug re-exposure. The aim was to assess the rate of subsequent EN recurrences such as re-exposure of high notoriety drugs in patients with EN. Methods: We used the French Health system database and included all EN patients. The primary outcome was the rate of EN recurrence. Secondary outcomes were high notoriety drugs re-exposure or cross exposure, i.e., exposure to a drug of the same family, after the acute phase of EN, when initially suspected. Results: A total of 1,203/1,440 patients (83.5%) survived the acute phase, with 27 patients (2.2% (CI95%: 1.5-3.2)) meeting the recurrence criteria. In patients with allopurinol as suspected drug, 10/77 (13.0%) were cross exposed to febuxostat, without recurrence. Similarly, in patients with carbamazepine/oxcarbazepine as suspected drug, 2/26 (7.6%) were cross exposed to lamotrigine, without recurrence. Conversely, 12/38 (31.5%) and 16/37 (43.2%) patients were respectively re-exposed to pantoprazole and esomeprazole when suspected, and 12/42 (28.6%) were re-exposed to amoxicillin. Only one recurrence was noted in a pantoprazole re-exposed patient. Conclusions: Among EN patients, the rate of recurrence seems low, contrasting with several re-exposures among beta lactam antibiotics and proton pump inhibitors, when suspected. Although we cannot exclude that the suspected drugs were not the responsible ones for several patients, future studies should assess the possible existence of transient risk factors inducing EN.

This is the first work to investigate the effectiveness of BERT-based contextual embeddings in active learning (AL) tasks on cold-start scenarios, where traditional fine-tuning is infeasible due to the absence of labeled data. Our primary contribution is the proposal of a more robust fine-tuning pipeline - DoTCAL - that diminishes the reliance on labeled data in AL using two steps: (1) fully leveraging unlabeled data through domain adaptation of the embeddings via masked language modeling and (2) further adjusting model weights using labeled data selected by AL. Our evaluation contrasts BERT-based embeddings with other prevalent text representation paradigms, including Bag of Words (BoW), Latent Semantic Indexing (LSI), and FastText, at two critical stages of the AL process: instance selection and classification. Experiments conducted on eight ATC benchmarks with varying AL budgets (number of labeled instances) and number of instances (about 5,000 to 300,000) demonstrates DoTCAL’s superior effectiveness, achieving up to a 33% improvement in Macro-F1, while reducing labeling efforts by half compared to the traditional one-step method. We also found that in several tasks, BoW and LSI (due to information aggregation) produce results superior (up to 59% ) to BERT, especially in low-budget scenarios and hard-to-classify tasks, which is quite surprising.

This study explores the convergence of homelessness and climate hazards within media and political discourse, focusing on three key questions: a) How did media outlets differ in their portrayal of clearing of the Zone, a downtown Phoenix encampment; b) to what extent did Phoenix’s Safe Outdoor Space feature in local media narratives of homelessness during this time; and c) how was exposure to environmental hazards, specifically heat and air pollution, included in the political and media narratives surrounding homelessness during the clearing of the Zone and creation of the Safe Outdoor Space? These questions are examined through a conceptual and empirical analysis of public discourse related to the Zone and the Safe Outdoor Space, drawing on data from Phoenix, Arizona’s local media outlets from January to December 2023. Our findings highlight the sociopolitical dynamics of narrative building around interventions for homelessness and underscore how environmental hazards are often an undertone—rather than a cornerstone—of media depictions of homelessness in the American Southwest.

This article presents a case study of a 31-year-old woman with a dual diagnosis of Borderline Personality Disorder (BPD) and Eating Disorder Not Otherwise Specified (EDNOS). Paula received a 12-month Dialectical Behavior Therapy (DBT) outpatient treatment. DBT is considered a transdiagnostic treatment approach to address emotion dysregulation, which shifts the therapy focus traditionally placed only on behavioral change towards including also validation and acceptance and dialectical strategies. DBT addresses eating symptomatology as a dysfunctional form of emotional regulation and has shown promising results regarding its efficacy for the treatment of BPD and EDNOS comorbidity. Given the growing evidence, a standard DBT treatment plan was developed for this case. Specifically, pre-treatment and phase 1 of the DBT program are described. During pre-treatment and phase 1, individual therapy aims to improve and maintain client’s motivation to change and engage in treatment, as well as to establish and prioritize treatment goals. As for group therapy, the main goal of the skills training in DBT is to enhance individual’s capability by increasing skillful behavior (mindfulness, emotion regulation, distress tolerance and interpersonal effectiveness skills). Paula received 24 weekly skills training sessions over a year. The results after a 12-month standard DBT treatment showed that Paula no longer met criteria for BPD, she had a significant decrease in difficulties in emotional regulation and impulsiveness and in EDNOS symptomatology. This case study may enhance learning about how to apply a transdiagnostic treatment to address BPD and EDNOS together in clinical practice.