A molecular descriptor, also known as a topological graph index, is a mathematical formula that may be implemented to any network that reflects a molecule structure. This index can be used to explore some physical aspects of a molecule and examine mathematical values. As a consequence, it is a better solution for costly and time-consuming laboratory experiments. In this work, we calculate the first temperature index, the second temperature index, the first hyper temperature index, the second hyper temperature index, the sum temperature index, the product temperature index, the reciprocal product temperature index and the $F$-temperature index. These defined indices are computed for the Non-KuKulean benzenoid graph.

Objective: To evaluate the learning curve and safety of total thoracoscopic mitral valve repair (MVP). Background: Total thoracoscopic MVP is characterized by minimal trauma, minimal bleeding, and short postoperative recovery time. The learning curve of any new procedure needs to be evaluated for learning and replication. However, minimally invasive mitral valve technique is a wide-ranging concept, no further analysis of the outcomes and learning curve of total thoracoscopic mitral valve repair has been performed. Methods: One hundred and fifty consecutive patients who underwent minimally invasive MVP alone without concurrent surgery were evaluated. Using Cardiopulmonary bypass (CPB) time and Aortic clamping (AC) time as evaluation variables, we visualized the learning curve for total thoracoscopic MVP using Cumulative sum analysis. We also analyzed important postoperative variables such as postoperative drainage, duration of mechanical ventilation, ICU stay and postoperative hospital stay. Results: The slope of the fitted curve was negative after 75 procedures, and the learning curve could be crossed after the completion of the 75th procedure when AC and CPB time were used as evaluation variables. And as the number of surgical cases increased, CPB, AC, postoperative drainage, duration of mechanical ventilation, ICU stay and postoperative hospital stay all showed different degrees of decrease. The incidence of postoperative adverse events is similar to conventional mitral valve repair. Conclusions: Compared to conventional MVP, total thoracoscopic MVP provides the same satisfactory surgical results and stabilization can be achieved gradually after completion of the 75th procedure.

The James R. Dolorio Water Reclamation Facility in Pueblo, Colorado, uses AvN aeration controls to lower aeration energy while promoting carbon-efficient nutrient removal and hydrocyclone-based wasting to achieve SVI improvements and process intensification. The results from the full-scale installation showed that hydrocyclone-based wasting helped improve settling characteristics by reducing the SVI from 200 ± 52 mL/g to 83 ± 22 mL/g within weeks of operation. PAO and nitrifiers were preferentially retained in dense flocs and granules, while lighter heterotrophic and filamentous organisms were preferentially wasted, thus uncoupling the SRT of these two fractions relative to overall SRT. The SRT of dense aggregates was estimated at 14.4 ± 3.4 days and for lighter flocs at 7.1 ± 2.3 days. The use of AvN control with continuous low DO conditions resulted in low DO conditions (< 0.4 mgO2/L) reducing air demands by 50% while providing excellent nitrogen (effluent TIN < 11 mg/L) and TP removal (effluent TP < 1 mg/L) at low primary effluent COD/N ratio of 6.0. The presence of comammox was demonstrated through molecular analysis, while ex-situ batch tests revealed the presence of DPAO, which could have attributed to the energy and carbon-efficient biological nutrient removal.

Gene splicing plays an extremely important role in the diversity of protein. In eukaryotic gene expression, and entirely eradication of introns and fusing of the remaining exons together is a prominent task because the exon sequence is mostly interrupted by introns. Owing to its importance in genetic engineering, it is extremely recommendable to identify these splicing sites. Through conventional experimental approaches, it becomes a difficult task, even in some situations impossible. The increasing genome sequences at an exponential rate in this area, it remained a challenge to develop a precise, reliable, and robust computational approach for fast prediction of splicing sites thus in the current study an ensemble space of Kmer, RevKmer, and Pseudo Trinucleotide composition (PseTNC) are applied to take out those features that can numerically describe the biological sample. Then these features were passed into three classification algorithms such as random forest, k-nearest neighbor, and support vector machine (SVM). After evaluation through the jackknife test, the proposed model achieved promising results of 93.92% and 96.39% for datasets S 1 and S 2 respectively. It has been noted that the identification performance of our current model (TargetSS) is better than the existing methods. Finally, we conclude that our proposed model for splicing site identification will be proved a useful tool for Bioinformatics, Computational Biology, Molecular Biology, and drug discovery applications.

The extraction of deeply impacted mandibular third molars could commonly cause the inferior alveolar nerve injury. This case is to share an innovative approach of coronectomy combined with segmental orthodontic appliances to remove impacted teeth which can reduce the risk of inferior alveolar nerve injury and improves periodontal regeneration.

Electron counting is an important concept that often governs properties such as stability of molecules, clusters, and complexes. Here we explore silver clusters doped with a transition-metal atom, where the behavior of d electrons has been an issue, i.e., whether or not they delocalize to participate in electron counting. The present study is performed on cations and anions of AgNM+/− (M = Sc–Ni) clusters to examine their stability through reaction with oxygen, which enables systematic control of the number of valence electrons in the system by the cluster size, the charge state, and the transition-metal element across the periodic table. Among 18-valence-electron clusters, both cations and anions exhibit exceptional stability upon Sc, Ti, and V doping, whereas only cations upon Fe, Co, and Ni doping; i.e., Ag16Sc+, Ag15Ti+, Ag14V+, Ag14Sc−, Ag13Ti−, Ag12V−, Ag11Fe+, Ag10Co+, and Ag9Ni+ are identified as magic clusters. The exceptional stability is attributed to electronic-shell closure with the 18 valence electrons, where 3d electrons are delocalized to contribute to electron counting. In contrast, Ag9Fe−, Ag8Co−, and Ag7Ni− are highly reactive even though they possess 18 valence electrons. Analysis of reaction products along with DFT calculations reveals that the transition-metal atom is endohedrally doped in the former magic clusters, whereas the doping is exohedral in the latter reactive species. It is thus found that an endohedral geometry plays a key role in delocalizing 3d electrons to participate in electron counting. Note that, upon Cr and Mn doping, the half-filled nature forces their 3d electrons to be localized both in endohedral Ag13Cr+ and Ag12Mn+ and in exohedral Ag11Cr− and Ag10Mn−.

In this paper, we provide the global well-posedness of the Cauchy problems of the 3d simplified Ericksen-Leslie system, modelling the incompressible nematic liquid crystal flows, with Fujita-Kato type initial data. More precisely, we prove that for any initial data $(u_0,d_0)\in H^{s}(\mathbb{R}^3)\times H^{s+1}(\mathbb{R}^3)$ with $s>\frac{5}{2}$, there exists a sufficient small $\varepsilon>0$ such that if the initial velocity $u_{0}$ satisfies \begin{align*} \|u_0\|_{\dot{H}^{\frac{1}{2}}}<\varepsilon \nu, \end{align*} and the initial orientation $d_0$ satisfies \begin{align*} \|d_0\|_{\dot{H}^{\frac{1}{2}}\cap\dot{H}^{\frac{3}{2}}}^2 \exp\Big\{ C\frac{\nu+\gamma}{\nu^2\gamma}(1+\|u_0\|_{\dot{H}^\frac{1}{2}}^2)\| u_0\|_{\dot{H}^\frac{1}{2}}^2 \Big\} <\varepsilon \min\{\nu,\gamma\}, \end{align*} for some positive constant $C$, the Ericksen-Leslie system admits a unique global solution $(u,d)\in C(\mathbb{R}_+; H^{s}(\mathbb{R}^3)\times H^{s+1}(\mathbb{R}^3))$.

The optimization of integrated coal gangue system of mining, dressing and backfilling in deep underground mining is a multi-objective and complex decision-making process, and the factors such as spatial layout, node location, transportation equipment need to be considered comprehensively. In order to realize the intellectualized location of the nodes of the logistics and transportation system of underground mining and dressing coal and gangue, this paper establishes the model of the logistics and transportation system of underground mining and dressing coal gangue, and analyzes the key factors of the intellectualized location of the logistics and transportation system of coal and gangue. In order to solve the problems of complex iterative update, slow running speed and poor stability of output results when particle swarm optimization (PSO) algorithm is used to solve the problem of node location, this paper proposes a particle swarm optimization and quasi-Newton algorithm (PSO-QNMs) for intellectualized node location of coal gangue system. By using MATLAB, this paper compares the calculation results of PSO algorithm and PSO-QNMs algorithm. The experimental results show that PSO-QNMs algorithm reduces the complexity of the calculation, increases the computational efficiency by 8 times, saves 42.8% of the cost, and improves the node optimization efficiency of the mining, dressing and backfilling system under the complex underground environment. Combined with the specific conditions of Xinjulong coal mine, the key nodes of underground coal and gangue system are located intelligently. The results prove that the method has high convergence speed and solving accuracy, which provides a basis for the optimization of mine logistics system in underground mining.

Introduction: During the COVID-19 pandemic, the risk of transmission of SARS-CoV-2 has not been precisely known in bronchoscopy procedures. We have designed a cabinet device called Ankara University Bronchoscopy Cabinet (Aubrocab®) to protect healthcare. In this study, we aimed to evaluate preventing effect of Aubrocab® on aerosol spreading by measuring the number of particles in the bronchoscopy suite. Methods: The patients were categorized into two groups as those who underwent bronchoscopy with and without Aubrocab®. We measured PM0.5 level just before and after the bronchoscopy procedure in the bronchoscopy suite. Results: A total of 82 patients, 62 of whom underwent bronchoscopy with Aubrocab®, were enrolled the study. The PM 0.5 level measured before bronchoscopy were similar in both groups, whereas the PM0.5 level measured just after bronchoscopy was significantly lower in the Aubrocab® group (42,603±8,632 vs 50,377±10,487, p=0.001). The analyses showed that the percent particle change (50.76 ± 19.91% vs 67.15 ± 24.24%, p=0.003) and the difference of the particle numbers between pre and post-procedure (13,638±4,292 and 19,501±5,891, p<0.001) were significantly lower in the Aubrocab® group. Conclusion: Our institution developed a barrier device named Aubrocab® which was shown to prevent excessive aerosol release in addition to routine precautions during bronchoscopy procedures.

Objective: The severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and the resulting COVID-19 pandemic pose significant challenges in terms of diagnosis and recurrent infections. Literature data suggest that age, gender and BMI factors are associated with immune response. The first aim of the study was to analyze the change in antibody titer at 15-day intervals until 60 days post symptom onset (PSO) The second aim was to analyze relationship between antibody titer and symptom grade, gender, age, BMI, therapeutic drugs, vitamin supplements, and herbal therapies. Materials and methods: Blood samples were collected from 43 patients (5 mild, 21 moderate, 17 severe diseases), 18 women (41.9 %), and 25 men (58.1 %), on 15, 30, 45, and 60 days PSO after COVID-19 infection. The serum antibody titers were determined by measuring the COVID-19 IgG antibodies by ELISA. Associations between the duration of symptoms, demographic and clinical parameters, medications and vitamins used, and herbal therapies were evaluated by interviewing the participants. Results: Within the first 15 days of illness, 81.4% of the patients were positive. From day 45 PSO, seropositivity was 89.5%. The anti-SARS-CoV-2 antibody titers were statistically higher in men than women at all-time (p<0.01). Antibody titer was higher in older participants compared to younger participants (p<0.02). Plaquenil or Favipiravir use did not effect antibody response (p>0.05). Men had higher fever (p=0.006), shortness of breath (p=0.004), and chest pain (p=0.03) than women. Conclusion: We found powerful antibody response by sixty days PSO, as well as higher antibody response and severity of symptoms in men gender. Data also showed that SARS-CoV-2 antibodies are higher in individuals with older age, whereas BMI, coexisting chronic disease, and drug used had no effect on antibody titers.

Cardiac failure due to sepsis is a common cause of death in patients with sepsis, but the specific mechanism leading to cardiac dysfunction is not clear. Therefore, we established a rat model of lipopolysaccharide (LPS)-induced sepsis-mediated cardiac dysfunction to investigate changes in the expression of miR-499 in the plasma and cardiac tissue and the potential mechanism. The results showed that after LPS stimulation, the expression of miR-499 in the plasma and myocardium was downregulated, the expression of α-myosin heavy chain (MHC) in the myocardium was significantly downregulated, and the expression of β-MHC in the myocardium was significantly upregulated. β-MHC/α-MHC dysregulation was reversed after miR-499 was overexpressed with the intravenous administration of a miR-499 agomir. An increased left ventricular ejection fraction (LVEF) , increased cardiac dysfunction and β-MHC/α-MHC dysregulation were observed after the injection of a miR-499 antagomir. These results suggest that miR-499 can improve sepsis-induced cardiac dysfunction in rats by regulating the expression levels of α-MHC and β-MHC, and targeting miR-499 could be an effective approach for treating sepsis-induced cardiac dysfunction.

Background: Juvenile temporal arteritis (JTA) is a rare manifestation of TA before 50-year- old. Objective: To describe a patient with juvenile temporal arteritis (JTA) and ocular involvement and excellent response to temporal excision. Case report: A 41-year-old male patient with an unmarked past medical history pain in his right eye with no change in visual acuity and sensitivity on the right cephalic temporal area. An angio-magnetic resonance imaging revealed a hyper signal on the right ophthalmic artery compatible with retinal vasculitis. Prednisone 40mg/day for 3 months was started, and no change in pain was noted. Laboratory tests were normal, except for antinuclear antibodies positive in a titer of 1:160 and a nuclear dense fine speckled pattern, with positive anti-LEDGF75 antibodies. A diagnosis of JTA was determined. A temporal artery was performed, with normal histology. After surgery, all symptoms disappeared. Conclusion: This article describes an additional JTA case who was successfully treated after temporal artery biopsy/excision.

Ultrasound is a non-invasive technique that has been shown to affect the cell proliferation, migration, differentiation, and promote the regeneration of cell. Our previous studies had proved that ultrasound improve natural killer (NK) cells mediated ovarian cancer therapy. To further explore the underlying mechanisms of ultrasound in NK cell and ovarian cancer cell, both cells treated by low-intensity pulsed ultrasound (LIPUS) and detected through ELISA analysis and western blot. The result demonstrated that the expression of Granzyme A, Granzyme B, perforin protein secreted by NK cells were increase. In the NK-SKOV3 co-culture system, the expression level of membrane permeability related proteins in the Wnt/β-catenin pathway of SKOV3 cells increased. This study presented cellular regulation in NK cells and ovarian cancer cells after LIPUS treatment and may expand the understanding of the complex molecular mechanism of LIPUS treatment on NK cells.

Accurately detecting steps in genetic diversity across landscapes is important for locating barriers to gene flow, identifying selectively important loci, and defining management units. However, there are many metrics that researchers could use to detect steps and little information on which might be the most robust. Our study aimed to determine the best measure/s for genetic step detection along linear gradients using biallelic single nucleotide polymorphism (SNP) data. We tested the ability to differentiate between linear and step-like gradients in genetic diversity, using a range of diversity measures derived from the q-profile, including allelic richness, Shannon Information, GST, and Jost-D, as well as Bray-Curtis dissimilarity. To determine the properties of each measure, we repeated simulations of different intensities of step and allele proportion ranges, with varying genome sample size, number of loci, and number of localities. We found that alpha diversity (within-locality) based measures were ineffective at detecting steps. Further, allelic richness-based beta (between-locality) measures (e.g., Jaccard and Sørensen dissimilarity) were not reliable for detecting steps, but instead detected departures from fixation. The beta diversity measures best able to detect steps were: Shannon Information based measures, GST based measures, a Jost-D related measure, and Bray-Curtis dissimilarity. No one measure was best overall, with a trade-off between those measures with high step detection sensitivity (GST and Bray-Curtis) and those that minimised false positives (a variant of Shannon Information). Therefore, when detecting steps, we recommend understanding the differences between measures and using a combination of approaches.

Root activity creates a unique microbial hotspot in the rhizosphere and profoundly regulates soil carbon (C) dynamics, but empirical assessments of the soil microbial carbon pump (MCP) and associated ecological consequences on soil C storage based on insight of the rhizosphere remains lack, especially for different root functional modules. Based on the assessment of MCP efficacy in the rhizosphere of absorptive and transport roots, we found that rhizosphere MCP efficacy was ~ 60%. The RMCP-efficacy was 26.5% higher in the rhizosphere of absorptive roots than in that of transport roots. The plant-derived C contributed only 5.71 ~ 10.48% of the increased SOC in the rhizosphere. These observations suggest that the soil MCP is intensely stimulated in the rhizosphere, especially in the rhizosphere of absorptive roots. Our study provides novel and direct empirical evidence for the active soil MCP functions in SOC sequestration from the perspective of the rhizosphere.

Aim- Imatinib is one of the tyrosine kinase inhibitors used for the treatment of chronic myeloid leukemia (CML) patients. The exact association of imatinib with anemia in CML patients is still unclear. The current study aimed to find the prevalence of anemia in chronic myeloid leukemia patients treated with imatinib. Methods- The relevant articles were searched in PubMed, Google scholar, and Clinical trials registries till 31st January 2021. The quality of the articles was assessed using the Newcastle-Ottawa Scale. The prevalence rate with 95% C. I was calculated using StatsDirect Statistical analysis software V.3. Results A total of 18 studies containing 3,537 patients (male-1912 and female-1478) were found relevant for the analysis. The pooled prevalence of anemia in CML was found to be 34% (95% CI: 23%-46%). However, the heterogeneity among studies was found to be high. Conclusion: The physician should also take care of anemia while treating CML patients with imatinib. Anemia is a major concern associated with CML patients treated with Imatinib.

Vascular endothelial growth factors (VEGF) regulate significant pathways in angiogenesis, myocardial and neuronal protection, metabolism, and cancer progression. There is growing evidence that the VEGF-B isoform is involved in cell survival, anti-apoptotic and antioxidant mechanisms, through binding to VEGF receptor 1 and neuropilin-1 (NRP-1). However the details of the interaction with NRP-1 and its functional importance have not been elucidated. We employed surface plasmon resonance technology and X-ray crystallography to characterize the direct binding between VEGF-B and the b1 domain of NRP-1, and developed VEGF-B – C-terminus derived peptides to be used as chemical tools for studying VEGF-B – NRP-1 related pathways. Results were compared with the corresponding VEGF-A derived peptides. Peptide lipidation was used as a means to stabilise the peptides. The binding of VEGF-B to NRP1-b1 was inhibited by a well characterized small molecule targeting the NRP1 arginine binding site. A crystal structure of a peptide with NRP-1 demonstrated that VEGF-B peptides bind at the canonical C-terminal Arginine binding site. VEGF-B – derived peptides containing a C-terminal arginine show potent binding to NRP1-b1 and inhibition of VEGF-A165 functional activity in HUVEC cells. Peptide lipidation increased binding residence time and improved plasma stability while maintaining binding affinity. The VEGF-B C-terminus imparts higher affinity for NRP-1 than the corresponding VEGF-A165 region. This binding is mediated through the canonical arginine site and the tight binding may impact on the activity and selectivity of the full-length protein. The VEGF-B167 derived peptides were more effective than VEGF-A165 peptides in blocking functional phosphorylation events.

Here we report a 5 year old girl with a new frameshift mutation (c.2318dup:p.S774Vfs*12) in the KMT2A gene.The patient’s clinical manifestations: postnatal growth retardation, early teething, rapid tooth replacement,dysplasia of dentition; wide eye spacing; generalized hirsutism; stubby fingers; low muscle tension and retarded mental development.

Vedolizumab is an immunosuppressive drug that acts locally on the gastrointestinal tract, is mainly used for the treatment of inflammatory bowel disease, and has been reported to be effective against gastrointestinal acute graft-versus-host disease (GI-aGVHD) in adults. However, there is insufficient evidence for pediatric GI-aGVHD. We used vedolizumab in three cases of GI-aGVHD aged 1.5-4.4 years. It was significantly effective in two cases and all cases had no serious side effects. Vedolizumab might be effective and safe for pediatric GI-aGVHD refractory to other treatments. Future study awaits.

The present paper gives an extraordinary view of the normal congruence of surfaces including the s lines and b lines in terms of electromagnetic wave vectors in ordinary space. Frenet Serret frame of given a space curve are described in E3 in terms of anholonomic coordinates which includes eight parameters. Using the expression the Frenet frames and electromagnetic wave vectors on the curve with a linear transformation in terms of each other, the changes of !t , !E and !B between any two points in the tangential and binormal direction along with the curved path = (s; n; b) are obtained in terms of geometric phase ; respectively. Intrinsic geometric properties of this normal congruence of surfaces are obtained in terms of electromagnetic wave vectors.

In this paper, a modified Leslie-Gower predator-prey model with strong Allee effect and double harvesting was studied analytically and numerically. The aim of the research is to provide an understanding of the impact of harvesting on the predator-prey model. The existence and stability of the equilibria was discussed, and we analyzed the existence of some bifurcations, such as saddle-node bifurcation, Hopf bifurcation and Bogdanov-Takens bifurcation. Furthermore, some numerical simulations were proposed to illustrate the dynamics of the model. Numerical results are consistent with the analytical results.

This paper introduces an accelerated uniformly convergent finite difference numerical approach for the singularly perturbed Burger-Huxley equation. The semi-linear property is treated with the help of the quasilinearization technique. A first-order finite difference approximation is applied in the temporal direction, and a nonstandard methodology of Mickens procedure is used in the spatial direction. To accelerate the rate of convergence from first to second-order, the Richardson extrapolation technique is applied. The convergence analysis of the proposed method has been established. Numerical experiments were conducted to support the theoretical results. Further, the result shows that the proposed method gives a more accurate solution than some existing methods in the literature.

Systemic lupus erythaematosus (SLE) is a chronic autoimmune disease associated with inflammation and organ damage. Based on accumulating evidence, activated T cells are key cells promoting the pathogenesis of SLE. Langchuangding (LCD) is an effective clinical traditional Chinese medicine prescription for treating SLE with few side effects and good patient compliance. LCD has proven great potential as a supplement to conventional treatments in previous clinical studies However, few studies have investigated its potential mechanism. Here, we prepared LCD-treated rat serum (LCDs) and evaluated their effects on activated Jurkat T cells stimulated with β-oestradiol in vitro. We developed a sensitive and reliable high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) method for the quality control of LCDs. T cell proliferation and apoptosis were detected with the CCK-8 assay and flow cytometry analysis. Caspase-3 mRNA and protein expression were detected using real-time PCR and western blotting, respectively. Two compounds, ferulic acid and isoferulic acid were simultaneously identify and their concentrations of high, middle and low doses of LCDs (H, M and L) were determined respectively. LCDs treatment inhibited the proliferation (P<0.05) of activated Jurkat T cells and induced apoptosis by up-regulating the mRNA and protein levels of caspase-3. Taken together, our results suggested that LCDs treatment depressed β-oestradiol-activated T cell proliferation by inducing apoptosis through caspase-3 activation, finally contributing to the amelioration of SLE. Thus, LCD potentially represents a promising therapeutic prescription for SLE supplement treatment with no adverse effects.

Objective: In the effective fight against SARS Cov-2 cases, which have started to be seen in our country since March 2020, the filiation studies conducted for resource detection and contact isolation facilitated the control of the epidemic and increased the success in epidemic control in the early period. After the definitive case detection, contact and close contact persons are detected by the filiation teams, and when necessary, these persons are tested and asymptomatic individuals are followed up by family physicians for 14 days. Method: For the evaluation, the Employee Satisfaction Scale and the Health Anxiety Scale (short version) Hospital Anxiety and Depression Scale (HAD), organized by the Ministry of Health, were used. Study data were collected using face-to-face interview technique. Results: Employee satisfaction in the pandemic was found to be moderate, and the risk of anxiety in women was higher than in men (p <0.05). There was no relatıon depression risk for both sexes (p> 0.05). Conclusions: Employee satisfaction in the pandemic was found to be moderate, and the risk of anxiety was higher in women than in men. No significant increase in depression risk has been reported in either sex. Multi-center studies with large participants are needed on this subject.