The nanomedicine and nanobiotechnology is a fast-developing field that has the potential to improve treatment regimens. The antidiabetic and antioxidant activity of iron oxide nanoparticles was investigated. This work focuses on the environmentally friendly manufacture of iron oxide nanoparticles (Fe 2O 3NPs) utilizing a polar extract of the root Azadirachta indica. The size, structural shape, absorption spectrum, and other pertinent characteristics of the produced nanoparticles were evaluated. The surface plasmon resonance (SPR) peaks at 231 nm were apparent in the UV-visible spectra, dynamic light scattering, scanning electron microscopy, and Fourier transform infrared spectroscopy were used for characterization. The antioxidant activity was validated approximately 50% DPPH inhibition, while the antidiabetic effect was approximately 65.26% suppression of α-amylase, surpassing the control’s performance. This biosynthetic approach offers a cost-effective, environmentally benign method with potential applications in various fields.

The purpose of this study was to assess and contrast the anti-diabetic properties of chitosan-silver oxide nanocomposite (CS-Ag 2O NC) and silver oxide nanoparticles (Ag 2O NPs). A green synthesis approach was used to create the nanoparticles and nanocomposite, The vitro models were used to assess the anti-diabetic effects. According to the findings, CS-Ag 2O nanocomposite demonstrated better glucose absorption, insulin sensitivity, and alpha-glucosidase inhibition than Ag 2ONPs, demonstrating greater anti-diabetic action. Additionally, the nanocomposite demonstrated enhanced biocompatibility and decreased toxicity. According to the study, CS-Ag 2O NC shows promise as a material for the creation of anti-diabetic medications. The nanoparticles and nanocomposite were characterized by UV- vis spectroscopy, infrared spectroscopy (FTIR) and, X ray diffraction (XRD) and EDAX analysis. The results showed a considerable Ag 2ONPs and CS-Ag 2O NC demonstrating that strong anti-diabetic potential