The purpose of this study was to assess and contrast the anti-diabetic properties of chitosan-silver oxide nanocomposite (CS-Ag ₂O NC) and silver oxide nanoparticles (Ag ₂O NPs). A green synthesis approach was used to create the nanoparticles and nanocomposite, The vitro models were used to assess the anti-diabetic effects. According to the findings, CS-Ag ₂O nanocomposite demonstrated better glucose absorption, insulin sensitivity, and alpha-glucosidase inhibition than Ag ₂ONPs, demonstrating greater anti-diabetic action. Additionally, the nanocomposite demonstrated enhanced biocompatibility and decreased toxicity. According to the study, CS-Ag ₂O NC shows promise as a material for the creation of anti-diabetic medications. The nanoparticles and nanocomposite were characterized by UV- vis spectroscopy, infrared spectroscopy (FTIR) and, X ray diffraction (XRD) and EDAX analysis. The results showed a considerable Ag ₂ONPs and CS-Ag ₂O NC demonstrating that strong anti-diabetic potential