Josh Vermaas V

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The thylakoid membrane is in a temperature-sensitive equilibrium that shifts repeatedly during the life cycle in response to ambient temperature or solar irradiance. Plants respond to seasonal temperature by changing their thylakoid lipid composition, while a more rapid mechanism for short-term heat exposure is required. The emission of the small organic molecule isoprene has been postulated as one such possible rapid mechanism. The protective mechanism of isoprene is not known, but some plants emit isoprene during periods of high-temperature stress. In this work, we investigate the dynamics and structure for lipids within a thylakoid membrane at different temperatures and varied isoprene content using classical molecular dynamics simulations. The results are compared with experimental findings from across the literature for temperature-dependent changes in the lipid composition and shape of thylakoids. We find that the surface area, volume, and flexibility of the membrane, as well as the lipid diffusion, increase with temperature, while the membrane thickness decreases. Saturated thylakoid 34:3 glycolipids derived from eukaryotic synthesis pathways exhibit significantly different dynamics than lipids from prokaryotic synthesis paths, which could explain the upregulation of specific lipid synthesis pathways at different temperatures. Increasing isoprene concentration was not observed to have a significant thermoprotective effect on the thylakoid membranes, and that isoprene readily permeated the membrane models tested here.

Luke Gregory

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OpinionPlant Cell & EnvironmentThe triose phosphate utilization limitation of photosynthetic rate: out of global models but important for leaf modelsLuke M. Gregory1,2, Alan M. McClain1,3,4, David M. Kramer1,3, Jeremy D. Pardo2,4, Kaila E. Smith1,2,4, Oliver L. Tessmer1, Berkley J.Walker1,2, Leonardo G. Ziccardi5, Thomas D. Sharkey1,31MSU-DOE Plant research Laboratory, Michigan State University, East Lansing, MI 48824 US A2 Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA3 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824 USA4 Plant Biotechnology for Health and Sustainability Program, Michigan State University, East Lansing, MI 48824 USA5 Department of Forestry, Michigan State University, East Lansing, MI 48824 USAXiao et al. (2021) present a method for estimating the variability of estimated parameters of the Farquhar, von Caemmerer, Berry (FvCB) model of photosynthesis (Farquhar et al., 1980). This model has been very effective at predicting photosynthetic responses to CO2, light, and temperature. The original model assumed one of two conditions: (1) rubisco is saturated with ribulose 1,5-bisphosphate (RuBP) and so responds to CO2 with Michalis Menten kinetics (with a competitive inhibitor/ second substrate oxygen) (rubisco-limited) or (2) rubisco uses RuBP as fast as it is made (RuBP regeneration-limted). In that case, rubisco activity is determined by the rate of RuBP regeneration, typically as a result of being light-limited. But even though photosynthetic CO2assimilation (A ) is light limited, it responds to increasing CO2 because of suppression of photorespiration. Carboxylation plus oxygenation stays constant under RuBP regeneration limited conditions so if oxygenation goes down as CO2increases, carboxylation will go up. The model was expanded to include a third condition, where RuBP regeneration is limited by how fast phosphorylated intermediates, primarily triose phosphates, are converted to end products, thereby releasing phosphate (Sharkey, 1985). This is usually called “triose phosphate utilization (TPU ) limitation.” Xiao et al. (2021) limited their analysis to rubisco-limited and RuBP-regeneration-limited fittings and said that TPU could also be included. We have tested how inclusion of TPU affects parameterization of the FvCB model.The model is most often parameterized by measuring CO2assimilation as a function of CO2 inside the air spaces of the leaf (Ci ), called anA /Ci curve. Rubisco-limited data points show a strong response to CO2 while RuBP-regeneration-limited points show less response but still increase with increasing CO2. TPU-limited points are characterized by no response to CO2 and sometimes an inhibition under increasing CO2. The condition is further diagnosed by a decline in photosynthetic electron transport caused by an increase in CO2 or decrease in O2 (measured by chlorophyll fluorescence analysis). The TPU limitation is rarely seen at physiological CO2partial pressure and temperature but is very frequently seen when CO2 is marginally higher than what the plant experienced during growth, especially if the temperature during the measurement is marginally lower than the growth temperature. TPU conditions are also associated with oscillations in photosynthetic rate (Sharkey et al., 1986), complicating measurements of TPU -limited photosynthesis rates.The parameters that can be estimated by the fitting models are the maximum rate of rubisco turnover (Vcmax ) and the rate of electron transport (J ) (since the analysis can be done at limiting light, this need not be Jmax ). Also estimated are respiration in the light (previously called day respiration) (RL ) and mesophyll conductance (gm ). If TPU is considered, this rate of triose phosphate use (TPU ). We have used equations proposed by Busch et al. (2018) to include carbon flow out of photorespiration as glycine (αG ) or serine (αS ).Some groups have concluded that TPU limitations are likely to be small and thus constitute an unnecessary complication for modeling photosynthesis at global scales (Kumarathunge et al., 2019; Rogers et al., 2021), and Xiao et al. (2021) also left TPU out of their recent analysis describing Bayesian methods for estimating parameters of the FvCB model and the uncertainties in those estimates. Given the obervations of declining A and photosynthetic electron transport in their data we believe ignoring TPU can lead to errors. We have systematically explored the consequences of including or ignoringTPU when parameterizing the FvCB model when TPU is apparent in the data.We began by re-analyzing the experimental data provided by Xiao et al. (2021). Four A /Ci curves measured with rice were provided. In three out of four cases, reverse sensitivity to CO2 of A was observed and in all four cases, photochemical yield (measured by chlorophyll fluorescence analysis) declined at high CO2. These behaviors indicate thatTPU was occurring. The authors specified in their methods section that they had to wait much longer for stability at the high CO2 concentrations and the data at high CO2 was noisy, also an indicator of TPU. We tested the effect of adding TPU to the analysis.We converted the most recent version (2.9) of the fitting spreadsheet that has been provided by Plant Cell and Environment (Sharkey, 2016) to an R script with a user-friendly interface (Shiny app), see  https://github.com/poales/msuRACiFit.The script iteratively fits data sets to biochemical models using rubisco-limited, RuBP-regeneration-limited, or TPU -limited assumptions, then calculates which process is likely to be rate-limiting for each data point, thus eliminating the need to assign specific limiting process to each of the data points.We then fitted the data supplied by Xiao et al. (2021), first withoutTPU and then with TPU (Figure 1). For all four curves supplied (only repetitions 2 and 3 are shown in Figure 1), includingTPU in the fitting improved the fit to the data at high CO2 and this was reflected in a reduction in the sum of the squared residuals (SSR) (data for repetitions 2 and 3 are given in Table 1). The reduction in SSRs was much greater than the increase in degrees of freedom introduced by including TPU as a fitting parameter.When data points are treated as J -limited but are actually limited by another process such as TPU , then J is likely to be underestimated. The estimate of J was higher whenTPU was included in the analysis (Table 1). Our fitting program could not estimate gm when TPU -limited points were treated as being J -limited and hit the limit imposed during fitting of 100 µmol m-2 s-1Pa-1. Because J -limited measurements hold the most information on mesophyll conductance, the estimate of mesophyll conductance is affected by fitting without TPU . When TPU is included it becomes clear how few data points are J -limited and since J -limited points have the most information aboutgm it becomes clear why gmcan be difficult to measure when A /Cicurves are measured at satuating light. Using high but not saturating light can increase the amount of J -limited data when estimatinggm (Sharkey, 2019)(see box 1).Three of the four A /Ci curves had noticeable discontinuity in the middle of the curves. We reasoned this was caused by the method used to make the measurements. It is common for researchers to report A /Ci curves assessed by measuring at 400 ppm CO2 and then measuring at a series of declining CO2 concentrations followed by a jump back to 400 ppm and measuring at a series of increasing CO2 concentrations. We call this the split method and it requires that photosynthesis be identical before and after measuring photosynthesis at ambient CO2, a requirement that often does not hold in our experience.We examined the effect of the sequence of CO2concentrations measured during an A /Cicurve and conclude that these measurements should be made by monotonic increasing (or decreasing) CO2 as opposed to starting at an ambient CO2 concentration and going down in CO2, jumping back to the middle and going up in CO2 (we call this the split method).We tested split versus monotonic methods with tobacco (Fig. 1 E and F). The curves did not show an obvious discontinuity but the SSR was higher for data generated by a split A /Ci curve than monotonic curve (Table 1) (These SSRs are comparable because the models used were the same and so the degrees of freedom did not differ.) Even when the curves do not show an obvious discontinuity in the middle when measured by the split method, results from “split” experiments tend to show stronger deviations from continuous fits to models, suggesting that hysteresis can strongly impact the interpretation. Moreover, the discontinuity comes at the section of the curve that has most information on mesophyll conductance and so significantly reduces confidence in mesophyll conductance values of such split curves.We conclude that 1. it is important to include TPU when fittingA /Ci curves that show evidence for it; 2.A /Ci curves should be carried out monotonically. 3. Additional data may be needed depending on how the fittings are to be used, for example it may be necessary to measure curves at saturating and also substaurating light to get robust measures of all parameters. Because of the danger of over fitting, when possible, parameters should be fixed. For example, if there are independent measures of mesophyll conductance or light respiration, these can be supplied and then fixed during fitting. It must be accepted that some parameters can change within minutes and this biological source of variance should be considered. Very rapid, monotonicA/Ci curves are likely to be very helpful in assessing the physiology of photosynthesis just as a high speed shutter on a camera helps bring things into focus, especially when the subject is dynamic.Reporting the parameters of the FvCB model can be helpful for global modeling, for detecting effects of the environment on photosynthesis, and changes in specific components of photosynthetic capacity. For large datasets fitting batches of curves using programs like R can be very helpful. What is presented expands on part of an earlier R Package (Duursma, 2015) but now includes TPU. The Shiny app allows users to test specific hypotheses and can be a convenient way to explore how changing conditions such as temperature and light affect predicted rates of photosynthesis.Please see  https://github.com/poales/msuRACiFitfor how to access and use the R-script and Shiny app used for this work.Funders: Division of Chemical Sciences, Geosciences and Biosciences, Office of Basic Energy Sciences of the United States Department of Energy (Grant DE-FG02-91ER20021).

Isaac Osei-Bonsu

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