AUTHOREA
Log in Sign Up Browse Preprints
LOG IN SIGN UP

Preprints

Explore 66,105 preprints on the Authorea Preprint Repository

A preprint on Authorea can be a complete scientific manuscript submitted to a journal, an essay, a whitepaper, or a blog post. Preprints on Authorea can contain datasets, code, figures, interactive visualizations and computational notebooks.
Read more about preprints.

ABCB1 c.3435C>T and EPHX1 c.416A>G polymorphisms influence plasma carbamazepine...
Ming-Liang Zhang
Fang-Zhou Liu

Ming-Liang Zhang

and 10 more

May 19, 2020
Background: ABCB1, EPHX1 and SCN1A gene polymorphisms have been reported play important roles in individual variability carbamazepine (CBZ) metabolism and resistance, but the result of that association still remains controversial. Objective: To clarify the associations among ABCB1, EPHX1 and SCN1A gene polymorphisms and CBZ metabolism and resistance. Methods: The PubMed, EMBASE, Cochrane library, Chinese National Knowledge Infrastructure, Chinese Science and Technique Journals Database, China Biology medicine disc and Wan Fang Database were searched for appropriate studies up to April 2020. Results: A total of 18 studies involving 3293 related epilepsy patients were included. ABCB1 c.3435C>T polymorphism was significantly associated with adjusted concentrations of CBZ (CC vs. CT, P=0.004), and EPHX c.416A>G polymorphism was significantly associated with carbamazepine-10, 11-trans dihydrodiol (CBZD) (AA vs. GG, P=0.045; AG vs. GG, P=0.010). Furthermore, ABCB1 c.3435C>T polymorphism was also observed to be significantly influenced CBZ resistance (CT vs TT, P=0.01; CC+CT vs TT, P=0.006). Conclusion: ABCB1 c.3435C>T polymorphism may affect the CBZ metabolism and resistance, EPHX1 c.416A>G polymorphism may only affect CBZ metabolism. These findings provided further evidence for individualized therapy of epilepsy patients in clinics. Nevertheless further large studies are still warranted to provide conclusive evidences.
Importance of Pulse Pressure after Extracorporeal Cardiopulmonary Resuscitation
Seok In Lee
Yong Su Lim

Seok In Lee

and 4 more

April 05, 2021
Background: Recent reports have revealed better clinical outcomes for extracorporeal cardiopulmonary resuscitation (ECPR) than conventional cardiopulmonary resuscitation (CPR).In this retrospective study, we attempted to identify predictors associated with successful weaning off extracorporeal membrane oxygenation (ECMO) support after ECPR. Methods: The demographic and clinical data of 30 ECPR patients aged over 18 years treated between August 2016 and January 2019 were analyzed. All clinical data were retrospectively collected. The primary endpoint was successful weaning from ECMO support after ECPR. Patients were divided into two groups based on successful or unsuccessful weaning off ECMO support (Weaned (n=14) vs. Failed (n=16)). Results: Univariate logistic regression analysis showed that age, CPR duration, ECMO complications, and loss of pulse pressure significantly predicted the results of weaning off ECMO support. However, multivariate logistic regression analysis showed that only CPR duration and loss of pulse pressure independently predicted unsuccessful weaning from ECMO support. Conclusion: We conclude that long CPR duration and loss of pulse pressure after ECPR predict unsuccessful weaning from ECMO. However, unlike CPR duration, loss of pulse pressure during post-ECPR was related to subsequent management. In patients with reduced pulse pressure after ECPR, careful management is warranted because this reduction is closely associated with unsuccessful weaning off ECMO support after ECPR.
Cryptic lineages respond differently to coral bleaching
Matias Gómez-Corrales
Carlos Prada

Matias Gómez-Corrales

and 1 more

May 19, 2020
Coral reefs are losing coral cover across the globe largely as a result of a rise in seawater temperatures that trigger coral bleaching and induce coral mortality. How coral reefs will respond to climate change will be a function of genetic variation and how it is partitioned among species. A critical initial step is to accurately delineate species and quantify their physiological potential to cope with heat stress. Cryptic species, morphologically indistinguishable but genetically different ones, typically harbor distinct physiological variation and respond differently to climatic changes. A dominant Caribbean reef builder severely affected by climate change is the mountainous star coral, Orbicella faveolata. Recently, Dziedzic et al. (2019) reported genetic variation in the physiological response to thermal stress in a single population of this species, suggesting that variation within populations will allow these corals to adapt to rising ocean temperatures. We reanalyzed their data and found multiple cryptic lineages rather than a single panmictic population, with only one of the lineages being heat-tolerant. Our finding of hidden lineages within a threatened species highlights the varying extinction risks faced by these independently evolving groups, especially when the prospects of survival under warmer oceans seem favorable for a few of them only.
Analytic First and Second Derivatives for the Fragment Molecular Orbital Method Combi...
中田浩弥
Dmitri Fedorov

hiroya nakata

and 1 more

May 19, 2020
Analytic first and second derivatives of the energy are developed for the fragment molecular orbital method interfaced with molecular mechanics in the electrostatic embedding scheme at the level of Hartree-Fock and density functional theory. The importance of the orbital response terms is demonstrated. The role of the electrostatic embedding upon molecular vibrations is analyzed, comparing force field and quantum-mechanical treatments for an ionic liquid and a solvated protein. The method is applied for 100 protein conformations sampled in MD to take into account the complexity of a flexible protein structure in solution, and a good agreement to experimental data is obtained: frequencies from an experimental IR spectrum are reproduced within 17 cm$^{-1}$.
Hypothalamic-pituitary-adrenal axis suppression in asthma: A glucocorticoid receptor...
Wisdom Akurugu
Carel van Heerden

Wisdom Akurugu

and 3 more

May 19, 2020
Background: Asthmatic children on corticosteroids can develop hypothalamic-pituitary-adrenal axis suppression (HPAS). Single nucleotide polymorphisms (SNPs) rs242941 and rs1876828 of the corticotrophin-releasing hormone receptor 1 (CRHR1) gene were associated with lower stimulated cortisol (F) levels, whereas rs41423247 of the glucocorticoid receptor (NR3C1) gene was associated with higher basal F levels. The objective of the current study was to confirm whether these three SNPs are associated with HPAS in asthmatic children. Methods: DNA was extracted from saliva obtained from 95 asthmatic children, who had previously undergone basal F and metyrapone testing. Thirty-six children were classified as suppressed. Non-suppressed children were sub-classified according to their post-metyrapone ACTH (PMTP ACTH) level into a middle (106-319 pg/ml) and a high (>319 pg/ml) ACTH response group. TaqMan® polymerase chain reaction assays were utilized. Results: Only rs41423247 was inversely associated with HPAS (OR = 0.27 [95% CI 0.06-0.90]). Its GC genotype was inversely associated with HPAS (log odds = - 1.28, p = 0.021). √PMTP ACTH was associated with CC (effect size = 10.85, p = 0.005) and GC genotypes (effect size = 4.06, p = 0.023). The C allele is inherited as a dominant trait (effect size = -1.31 (95% CI -2.39 – -0.33; p = 0.012). In the high ACTH response group, both genotypes affected the PMTP ACTH (effect sizes 1.41 and 15.46; p-values 0.023 and < 2x10-26 for GC and CC respectively). Conclusions: The C allele of rs41423247 was found to be protective against HPAS. CC genotype is associated with the highest PMTP ACTH response.
Is electronic monitoring of adherence to inhaled steroids useful in troublesome presc...
Yvonne Bingham
James Cook

Yvonne Bingham

and 7 more

May 19, 2020
Background: Adherence to inhaled corticosteroids (ICS) is suboptimal in school-age children with asthma. Little is known about adherence to ICS in preschool children with troublesome wheeze. Children with aeroallergen sensitisation, or those with multiple trigger wheeze (MTW), should be differential ICS responders. We hypothesised that adherence to ICS and symptom control are only positively related in atopic children, or those with MTW. Methods: Patients aged 1-5 years with recurrent wheeze prescribed ICS were recruited from a tertiary respiratory clinic. Clinical phenotype and aeroallergen sensitisation were determined, and adherence assessed using an electronic monitoring device (Smartinhaler®). Symptom control (Test for Respiratory and Asthma Control in Kids (TRACK)), quality of life (PACQLQ), airway inflammation (offline exhaled nitric oxide (FeNO)) were assessed at baseline and follow-up. Results: Forty-eight children (mean age 3.7 years, SD 1.2) were monitored for a median of 112 (IQR 91-126) days. At baseline n=29 had episodic viral wheeze (EVW) and n= 19 had MTW. 24/48 (50%) wheezers had sub-optimal ICS adherence (<80%). Median adherence was 64% (IQR 38-84). There was a significant increase in TRACK in the group as a whole, unrelated to adherence. When split according to ICS responders, a significant increase in TRACK was only seen in atopic wheezers with adherence >60%. There was no relationship between clinical phenotype, adherence and symptom control. Conclusion: Adherence to ICS was sub-optimal and was positively related to symptom control in atopic wheezers only. Assessments of adherence are important in preschool children with an ICS responsive phenotype prior to therapy escalation.
STUDY OF EPITHELIAL CELL GENES IN A SAMPLE OF EGYPTIAN CHILDREN WITH BRONCHIAL ASTHMA
Ola  Behairy
osama mohammad

Ola Behairy

and 3 more

May 19, 2020
Objective: to assess epithelial cell genes (TMEM178, FKBP5, CLCA1, SERPINB2 and Periostin) in childhood asthma and their utility in predicting asthma severity, level of control and atopic status. Study design: 70 stable asthmatic children included who were further subdivided into mild, moderate and severe persistent asthma, also subdivided into controlled and partially to uncontrolled asthma and 30 apparently healthy children. All children were subjected to medical history taking, clinical examination, complete blood count, serum IgE, and nasal epithelial samples were collected for detection of epithelial cell genes (TMEM178, FKBP5, CLCA1, SERPINB2 and Periostin) by real-time PCR. Results: TMEM178 showed significant down regulation in asthmatic children and its expression levels decreased significantly with the progression of asthma severity. CLCA1, SERPINB2 and Periostin showed statistically significant up regulation in asthmatic children with no statistically significant differences between different degrees of asthma severity. FKBP5 showed neither statistically significant difference with control group nor between different degrees of asthma severity. TMEM178, CLCA1, SERPINB2 and Periostin were significantly up regulated in controlled asthma. While, FKBP5 was significantly up regulated in partially to uncontrolled group. CLCA1, SERPINB2 and Periostin were significantly up regulated in atopic asthma while TMEM178 and FKBP5 showed no statistically significant differences between atopic and non-atopic asthma. Conclusion: TMEM178 expression gained attention as a predictor of asthma severity. CLCA1, SERPINB2 and Periostin expression were upregulated not only in airway epithelial cells of asthmatic children but also in controlled and atopic asthma, whereas FKBP5 was upregulated in partially to uncontrolled asthma.
Novel mutation c.1224_1225insGTCC (p.Cys409Valfs*41) of MEN1 gene in a multiple endoc...
Paula Molina
Ernesto Ruiz

Paula Molina

and 3 more

May 18, 2020
Abstract Multiple endocrine neoplasia type 1 (MEN1) is a rare autosomal dominant disorder characterized by a predisposition for tumors in the parathyroid gland, anterior hypophysis and pancreatic islet cells. Here we describe the first Costa Rican MEN1 case with a novel MEN1 mutation in a 37-year-old male with history of nephrolithiasis and recurrent hypoglycemic episodes. Initial laboratories revealed hypoglycemia and primary hyperparathyroidism. A fasting test was positive at three hours demonstrating hyperinsulinemic hypoglycemia. An abdominal CT SCAN showed one mass in the pancreatic tail and bilateral nephrolithiasis. Direct sequence analysis of MEN1 gene revealed heterozygosity for a novel pathogenic insertion c.1224_1225insGTCC (p.Cys409Valfs*41). Early genetic diagnosis of MEN1 could identify individuals in a presymptomatic stage of the disease which could introduce them in periodic surveillance programs for early identification and treatment of associated tumors. Key words Multiple endocrine neoplasia, primary hyperparathyroidism, insulinoma, hypoglycemia, nephrolithiasis
Electrostatic Immobilization of Ionic Liquids onto SBA-15 as Heterogenized Catalysts...
Nasser Shalaby
Hanan Ahmed

Nasser Shalaby

and 4 more

May 18, 2020
To overcome the drawbacks of homogeneous catalysts in the synthesis of trimethylolpropane trioleate (TMPTO), ionic liquids with different acidity were electrostatically immobilized onto SBA-15 to obtain heterogenized catalysts. The catalytic performance of the as-prepared two catalysts (IL1/SBA-15 and IL2/SBA-15) was investigated at different temperature (100 -140oC) and molar ratio of oleic acid (OA) to trimethylolpropane (TMP) (3:1 to 5:1) at fixed catalyst dosage of 3 wt. % and reaction time of 20h; where the catalyst IL1/SBA-15 with higher acidity exhibits higher activity. The optimum temperature (at OA: TMP = 3: 1) was obtained at 130oC, where the total conversion was 86.1% and 70% with selectivity towards TMPTO of 95.6% and 92.96% for IL1/SBA-15 and IL2/SBA-15, respectively. Above this temperature, the selectivity began to decrease. The effect of OA: TMP molar ratio showed the increase of conversion (92.7% and 79.1%) with the increase of OA: TMP molar ratio up to 4.5. Further increase in OA concentration results in a damping of the catalytic efficiency of the two catalysts. Through six consecutive runs, the periodicity test refers to the relative stability of the employed catalysts with slightly decrease in activity. All results were interpreted and discussed in detail
Production of Protein Isolates from Chilean Granado Bean (Phaseolus vulgaris L.)
Priyanka Kumar
Bih King Chen

Priyanka Kumar

and 5 more

May 18, 2020
The Chilean granado bean (Phaseolus vulgaris L.) contains nutritionally valuable proteins, and there was indication that the proteins can help in the prevention of diabetes. To further explore tis potential purified samples of the bean proteins is required. A membrane-based process was developed for the isolation of proteins from granado beans, adapted from methods reported earlier for mustard protein processing. The optimised process consists of alkaline protein extraction from granado bean flour at pH 10, ultrafiltration at concentration factor 4 and diafiltration with diavolume 4 followed by isoelectric protein precipitation at pH 4. The process starting with granado beans containing 28% protein, recovered 60.1% of the protein as precipitated protein isolate (PPI) and 7.2% as acid soluble protein isolates (SPI). The losses in the process system were approximately 26% of mass and 18.8% of nitrogen due to removal of non-protein nitrogen and small molecular weight components, likely carbohydrates. The protein contents of the PPI and SPI were ~92 % and ~ 62% on a moisture-free basis; the protein content of the SPI produced is considerably lower than typical isolates. This may be due to the co-recovery of high molecular weight carbohydrates. The water absorption capacity and nitrogen solubility index, of the PPI and SPI were measured and compared to other oilseed isolates. The PPI showed high water absorption (<400%). SPI dissolved completely – a nitrogen solubility index of 100%, while PPI had low nitrogen solubility near its isoelectric point. Both isolates had traits desirable for easy incorporation into food products.
    Estrategias de la mercadotecnia y recursos humanos en la globalización  
América Hernández- Grijalva
Griselda Lazalde Solis

América Hernández- Grijalva

and 2 more

October 28, 2020
Antecedentes De acuerdo con  \cite{mera2005globalizacion}  La globalización ha cambiado las formas de la competencia empresarial, que ahora están dominadas por los activos intangibles y por las personas que, o bien los componen o bien los utilizan. Por ello, las empresas que actúan globalmente prestan atención especial a la dirección de sus Recursos Humanos, es decir que los toman como un componente estratégico y, al mismo tiempo, su consideración como personas; esto es, atender a su dimensión ética y moral. Para ello se propone como primer paso de un camino que no es exclusivo ni excluyente, el empleo de certificaciones sobre Recursos Humanos. De acuerdo con \cite{aranguren2006gestion}  La globalización es un fenómeno contemporáneo que implica retos importantes para las empresas, pues deben adaptarse a condiciones cambiantes que amenazan permanentemente su competitividad y posicionamiento en los mercados locales, nacionales o internacionales. No obstante, con frecuencia se presentan diversas manifestaciones definidas por muchos autores como flexibilización, achatamiento, exrternalización, entre otras, las cuales en muchos casos desembocan en fraude laboral. A la gestión de los recursos humanos, partícipe de este proceso, le corresponde implementar los cambios en su materia, aunque probablemente su participación en la toma de decisiones haya sido muy limitada o en el peor de los casos, nula. Esto plantea un reto a las formas actuales de gestionar recursos humanos, que buscan por un lado, el aumento de la productividad y por el otro, el aumento de la calidad de vida en el trabajo, siempre dentro de los preceptos éticos de acuerdo a los nuevos enfoques de corte humanista, bajo el supuesto de que si se mejora el nivel de vida en el trabajo, se contará con un trabajador más sano, dispuesto, motivado y, por tanto, productivo.
Discrete Multiplicative Difference Duality
Benedict Irwin

Benedict Irwin

March 13, 2026
ABSTRACT We investigate an apparently fundamental operation on commonly occurring mathematical series. MAIN If we take the hypergeometric series for example: \;_2F_1(a,b,c,x) = ^\infty {(c)_k}{k!} we can notice that for the transform $$ _n[f(n)](m) = ^m f(n) = g(m) $$ which is almost the indefinite product, we have the inverse transform of the summation kernel $$ ^{-1}_k \left[{(c)_k}{k!}\right](n) = {(n+c-1)}{n} $$ we now proceed to define a generating function of this new kernel as G(t) = ^\infty {(n+c-1)}{n} t^n with $$ G(t) = _1(2,c;c+1;t))}{c-1} $$ for an example of $f(x) = {\pi}K(x)$ with a = b = 1/2, c = 1, then $$ G(t) = _2(t)}{4}-{t-1}+x \log (1-t) $$ we have that the inverse Z-transform of $G({t})$ gives $$ ^{-1}\left[G({t})\right] = {4n^2}, n>0 $$ and we can re-extrude this as $$ \prod_n {4n^2} = {2}+n)x^n}{\pi \Gamma(1 + n)^2} $$ yielding $$ ^\infty ^n {4n^2} = {\pi}K(x) $$ TRANSFORM From this we essentially have an operator $_x$ that maps a function to the discrete difference reduced term, for the hypergeometric example this would be $$ _x[\,_2F_1(a,b,c,x)] = {(n+c-1)}{n}. $$ The inverse operation $^{-1}$ is then exactly $$ ^{-1}_n[\square](x) = ^\infty ^m x \square $$ this is used as $$ ^{-1}_n[f(n)](x) = ^\infty ^m x f(n) $$ this means the operator Q is a composition of “coefficient of” operator, commonly denoted [xn] and the discrete difference derivative Δn* as $$ _x[\square](n) = \Delta^*_n [x^n] \square $$ CONNECTION TO MELLIN TRANSFORM We can connect this to the Mellin transform and the Ramanujan master theorem, which essentially extracts coefficients. For a function f(x) = ^\infty {k!} \phi(k) x^k we have that the Mellin transform is related to the coefficient function by $$ [f](s) = \Gamma(s)\phi(-s) $$ for suitable functions. In effect this becomes the method of coefficient extraction, but brings a sign flip, σ, operation in. Thus for a function defined as in equation [eqn:RMT] we have $$ Q f = \Delta^* \sigma {\Gamma(s)} ^{-1} f $$ then an operator G would indicate summing over positive non-zero integers $$ G_n[\square](t) = ^\infty t^n \square $$ some important identities that are not immediately obvious when reducing more complex series expansions such as elliptic integrals ^n {(2n-2)!} = \Gamma(2n+1) \\ ^n {(mn-m)!} = \Gamma(mn+1) \\ ^n {(mn-m+b)!} = {b!} \\ ^n {1-2n} = {1-2n} EXAMPLES Transforms from function to generating function: G Q[e^x] = -\log(1-t) \\ G Q[e^{-x}] = \log(1-t) \\ GQ\left[{1-x}\right] = {1-t} \\ GQ\left[{1+x}\right] = -{1-t} \\ GQ\left[I_0()\right] = _2(t)}{4} \\ GQ\left[I_0()\right] = _2(t)}{4} \\ GQ\left[J_0()\right] = -_2(t)}{4} \\ GQ[ {\pi} K(x)] = _2(t)}{4}-{t-1}+\log (1-t) \\ GQ[ {\pi} E(x)] = _2(t)}{4}+{1-t}+2\log (1-t) \\ GQ[ )}{}] = 3 + {1-t} - 4 {}}{} - {2}\log(1-t) \\ GQ[ 3)/3)}{}] = -{t-1}-{9} \log (1-t)-\left(\right)}{9 }+{9} \\ GQ\left[(1-x)^{-5/9}\right] = {1-t} + {9} \log(1-t) \\ GQ\left[(1-x)^{a-1}\right] = {1-t} + a \log(1-t)\\ GQ\left[1-\tanh^{-1}()\right] = {1-t} - 2 \tanh^{-1}() \\ \cosh() \to \tanh^{-1}() + {2}\log(1-t) \\ \cos() \to - \tanh^{-1}() - {2}\log(1-t) \\ ^\infty {(3k)!} \to {2} \, _2F_1\left({3},1;{3};t\right)-{2} \, _2F_1\left({3},1;{3};t\right)-{6} \log (1-t) here we see that $$ GQ\left[ {\pi} K(x)\right] = GQ\left[I_0()\right] + GQ\left[{1+x}\right] + G Q[e^{-x}] $$ There could be some secret equivalence between the function on the left and that on the right. I.e. the elliptic K function may transform under an operator and the combination of functions on the right may transform in analogy under a different operator. For example $$ tD_t \pm \log(1-t) \to {1-t} $$ so this meta derivative converts $$ e^{\pm x} \to {1\pm x} $$ this can be seen to be similar to an inverse Borel transform! From the above list of transforms it is clear that we see repeating units or “elements”, for example log(1 − t) is very common. It may be instructive to find a naming system for these units to give a compact representation of the resulting function. Whether these elements form some kind of basis for the underlying function space is yet to be investigated. We appear to have functions of the form x ₂F₁(a, b, c, x), or at least for shorthand -\log(1-t) = t\;_2F_1(1,1,2,t) = t_{1,1;2} \\ \sin^{-1}() = t_{{2},{2};{2}} with this we can immediately see $$ ^\infty {(3k)!} \to {3},1;{3}}}{2}-{3},1;{3}}}{2}+}{6} = {2} & -{2} & {6} t_{{3},1;{3}} \\ t_{{3},1;{3}} \\ t_{1,1;2} $$ important terms might include $$ ^\infty H_n t^n = -{1-t} = }{1-t} $$ to handle this we would need to evaluate $$ ^n H_k = f(n) $$ and apparently little is understood about these terms in OEIS A097423 and A097424. DERIVATIVES Consider the derivative of a sequence, we have $$ {dx} ^\infty a_k x^k = ^\infty (k+1)a_{k+1}x^k $$ where we have made sure to keep the sequence index from 0 to ∞. We can write $$ ^k {n} = k+1 $$ which tells us $$ Q[f'(x)] = ^\infty {n} \Delta^*_k[a_{k+1}](n) t^n $$ for example if for ex we have ak = 1/k!, then the derivative gives $$ Q[e^x] = ^\infty {n} \Delta^*_k[{k!}](n) t^n $$ and $\Delta^*_k[{k!}](n) = (n+1)^{-1}$ which consistently gives $$ Q[e^x] = ^\infty {n} t^n = -\log(1-t) $$ this is powerful, and we can use this to calculate unknown derivatives Δk*, and potentially solve differential equations in a mirror domain. In general we have a beautiful relationship $$ {dx^n} ^\infty a_k x^k = ^\infty (k+1)_n a_{k+n}x^k $$ for ex this means $$ \Delta^*_k[{\Gamma(k+n+1)}]= \Delta^*_k[{\Gamma(k+1)}] = {n} $$
Genome analysis of Getah virus (GETV) isolated from a live swine vaccine: The commerc...
Feng Zhou
Aojie Wang

Feng Zhou

and 6 more

May 18, 2020
We reported a GETV strain, named GETV-V1, was isolated from a commercial modified live vaccine (MLV) against porcine reproductive and respiratory syndrome virus (PRRSV), which is widely used on pigs in China. Further results showed that nine batches of MLV vaccine (three batches per year) from the same manufacturer between 2015 and 2017 were all positive for GETV. The complete genomes of the isolate were sequenced and it was found that the genomes of GETV-V1 had the highest similarities with a strain 16-I-674, which was isolated from the sick horses in Japan. This is the first report of GETV contaminant in live swine vaccine in China. Our finding demonstrates that vaccination of the commercial live vaccines might be contaminated and became a potential new route for GETV transmission in swine, pointing to the need for more extensive monitoring of the commercial live vaccines in China.
Root-derived inputs are major contributors to soil carbon in temperate forests
Adrienne Keller
Edward Brzostek

Adrienne Keller

and 4 more

May 18, 2020
Roots promote the formation of slow-cycling soil carbon (C), yet we have limited understanding of the magnitude and controls on this flux. We hypothesized that root-derived inputs from ectomycorrhizal (ECM)-associated trees would be greater than those from arbuscular mycorrhizal (AM)-associated trees, and that soils receiving the greatest inputs would promote greater root-derived C accumulation in mineral-associated pools. We installed δ13C-enriched ingrowth cores across mycorrhizal gradients in six Eastern U.S. forests (n = 54 plots). Counter to our hypothesis, root-derived C was 54% greater in AM versus ECM-dominated plots, resulting in 175% more root-derived C in mineral-associated, slow-cycling pools in AM compared to ECM plots. Notably, root-derived soil C was comparable in magnitude to leaf litter inputs and aboveground net primary production. Our results suggest that variation in root-derived C inputs due to tree mycorrhizal dominance may be a key control of soil C dynamics in forests.
Novel mutations A210G and L15Q TBX5 gene in the patients with non-syndromic congenita...
fariborz soheili

FS

May 18, 2020
The TBX5 transcription factor plays an important role during morphogenesis and development of the heart. Mutations in this gene often lead to Holt-Oram syndrome (HOS). This study identified mutations in patients with non-syndromic congenital heart defects (CHD). Screening for mutations TBX5 gene in non-syndromic CHD, including 100 patients with a septal defect and 50 healthy subjects as controls were performed by the technique of high-resolution melt (HRM). Exons were sequenced for samples that showed HRM curve differences compared to controls. Structural stability and pathogenic potential of mutated protein were evaluated by bioinformatics analysis. HRM curve analysis showed that the curves of three samples deviated from the curves of controls. Sequencing showed three heterozygous missense mutations including two novel mutations NM_000192.3:c.44T>G, (p.L15Q), NM_000192.3c.629C>G (p.A210G) and a known mutation NM_000192.3:c.331G>T (p.D111Y). The PolyPhen-2 software predicted the p.D111Y and p.A210G substitutions to be disease-causing and p.L15Q as possibly benign, while protein structural stability analysis by MUpro and DynaMut suggested that these mutations reduce stability and increase the flexibility of the protein. This study presents two novel missense mutations within the TBX5 gene that may be causal for non-syndrome CHD.
Burden of Rare Deleterious Variants in WNT Signaling Genes Among 511 Myelomeningocele...
Luke Hebert
Paul Hillman

Luke Hebert

and 8 more

May 18, 2020
Genes in the noncanonical WNT signaling pathway controlling planar cell polarity have been linked to the neural tube defect myelomeningocele. We hypothesized that some genes in the WNT signaling network have a higher mutational burden in myelomeningocele subjects than in control subjects. Exome sequencing data from 511 myelomeningocele subjects was obtained in-house and data from 29,940 ethnically matched subjects was provided by version 2 of the publicly available Genome Aggregation Database. To compare mutational burden, we collapsed rare deleterious variants across each of 523 human WNT signaling genes in case and control populations. Ten WNT signaling genes were disrupted with a higher mutational burden among Mexican American myelomeningocele subjects compared to Hispanic controls (Fishers exact test, P ≤ 0.05) and seven different genes were disrupted among individuals of European ancestry compared to controls. Gene ontology enrichment analyses indicate that genes disrupted only in the Mexican American population play a role in planar cell polarity whereas genes identified in both populations are important for the regulation of canonical WNT signaling. In summary, evidence for WNT signaling genes that may contribute to myelomeningocele in humans is presented and discussed.
Spike development inhibition in the ftin mutant is associated with multiple phenotypi...
Yong-sheng Zheng
Jinpeng Zhang

Yong-sheng Zheng

and 7 more

May 18, 2020
Spike development of wheat is closely associated with the ability of response to cold stress and inhibited under cold stress in spring. Morphological investigation showed that the ftin gene in 3558M is associated with multiple phenotypic characteristics, including fewer tillers, delayed floral transition, and the death of shoot apical meristem. In this study, we systematically researched the genetic nature of spike development using ITRAQ, transcriptome sequencing, western blot and RNAi technologies. The results showed that the ftin mutant is cold sensitive and activates the cold acclimation pathway. Multiple defence responses, including the ROS-mediated hypersensitive response, SA-mediated systemic acquired resistance and FLS2-induced pathogen-associated defence response, were activated to respond to normal cold stress and led to the apex death. Meanwhile, the continuous cold acclimation pathway inhibited the SVP-SCO1-LFY flowering pathway and leads to inhibition of spike development. Two TaPIN proteins were significantly downregulated, and multiple auxin signalling genes were also differentially expressed after cold stress. We used RNAi technology to knock down the two TaPIN genes and the tiller number was significantly reduced in TaPINa-RNAi and TaPINb-RNAi wheat. Taken together, the results revealed that the ftin gene might directly or indirectly regulate multiple biological pathways to affect multiple phenotypic characteristics.
Effect of 20 years of mineral and organic fertilization on CO2 and N2 fixation bacter...
Caixia Liu
Linhua Cao

Caixia Liu

and 6 more

May 18, 2020
The fixation of atmospheric CO2 and N2 by soil bacteria is important to the terrestrial carbon (C) and nitrogen (N) cycles and can be greatly affected by anthropogenic disturbance. The application of mineral fertilizer combined with manure (MCM) instead of MF have been used to mitigate soil degradation caused by long term mineral fertilizer (MF) practiced in widely distributed Moso bamboo. We conducted an investigation, employing the statistical approach of space-for-time substitution, to evaluate the effect of MCM on soil CO2 and N2 fixation bacteria in subtropical region of China. Intensively managed Moso bamboo plantations receiving MCM for 0, 6, 10, 15, and 20 years were examined. MCM management enhanced the content of soil organic C and available soil N, P, K, although some fluctuating values were observed. Soil pH values were generally stable, ranging between 5.0 and 5.5. The abundance of the genes cbbL and nifH decreased significantlyafter 6 years of IM and then gradually recovered. Both CO2 and N2 fixation bacteria have their similar dominant species, such as Bradyrhizobium, but these differed in their relative abundances among treatments. The diversity of both bacterial groups either decreased or increased at the 10-year sampling and later returned to their original levels. The alterations of abundances and measures of community diversity for both CO2 and N2 fixation bacteria were not driven by unique factor. In conclusion, IM practice with MCM in Moso bamboo plantation had an overall positive effect on soil CO2- and N2-fixing bacteria as well as soil properties.
Drought and re-watering modify ethylene production and sensitivity, and are associate...
Andre Lima
Iasminy Santos

Andre Lima

and 8 more

May 18, 2020
Coffee flowering requires a period of water deficit followed by rainfall to break flower bud dormancy and promote anthesis. Since drought followed by re-watering can increase shoot ethylene production, we investigated changes in root, leaf and flower bud ethylene production and expression of genes within the ethylene biosynthesis and signalling pathways and their relationship to coffee flowering. Drought decreased foliar and flower bud ethylene production without changing root ethylene production, even though all tissues likely accumulated the ethylene precursor ACC (1-aminocyclopropane-1-carboxylic acid), since ACS gene expression was maintained while ACO gene expression decreased. The ethylene receptor CaETR4-like was not differentially expressed in leaves under water deficit, but it was downregulated in roots. Re-watering restored shoot ethylene production, which seems important in promoting anthesis. 1-MCP, an ethylene action inhibitor, triggered coffee anthesis without re-watering the plants, which hitherto was considered essential to allow flowering. 1-MCP positively regulated ethylene biosynthesis genes (CaACS1-like and CaACO1-like), similar to re-watering, and downregulated CaETR4-like, suggesting that changes in ethylene levels and sensitivity are required to promote coffee anthesis. Thus, drought and re-watering-induced changes in ethylene levels and sensitivity allow coffee flowering, while the growth regulator 1-MCP can potentially regulate anthesis time and intensity.
Genome-Wide Identification of Grapevine's C3H2C3 Type RING E3 Ubiquitin Ligases Famil...
Yihe Yu
Shengdi Yang

Yihe Yu

and 6 more

May 18, 2020
RING is one of the largest E3 ubiquitin ligase families, playing an important role in plants’ development and growth and their biotic and abiotic stress responses. The C3H2C3 type is the largest subfamily of RING. Here, 143 C3H2C3-type RING genes (RCHCs) were discovered from the grapevine genome and separated into groups (I~XI) according to their phylogenetic analysis, with these genes named according to their positions on chromosomes. Gene replication analysis showed that 26 group segmental duplications and 14 tandem duplications of VyRCHCs were identified. All VyRCHCs have introns, VyRCHC29 has a maximum of 19 introns and 96 VyRCHCs have introns of 1 to 3. ARE, ABRE and O2 site Cis-acting elements very conservative in the promoter of VyRCHCs, with a large number. The expression profiles of eight DEGs in RNA-Seq after drought stress were similar to those in qRT-PCR analysis. The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, while Arabidopsis thaliana overexpressing VyRCHC114 had stronger tolerance of drought stress than the control. Valuable new information on the evolution of grapevine RCHC family genes and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress emerged from this research.
Effects of physical activity on quality of life of coronary artery bypass grafted pat...
ying mao
Ling sun

ying mao

and 2 more

May 18, 2020
Objective: Coronary artery disease (CAD) is associated with remodeling and narrowing of the coronary arteries and is the primary cause of mortality and handicap around the globe. Coronary artery bypass grafting (CABG) is an established method for the treatment and symptomatic relief of severe form of CAD. Post-GABG patients experience poorer quality of life and worse long-term psychological outcomes. This study systemically evaluates the effects of physical activity intervention on the quality of life of post GABG patients. Methods: Two randomized controlled trials recruiting 120 post-CABG patients met the inclusion criteria and were systemically analyzed. Endpoints measured in this study included physical and mental components related to quality of life. Data was analyzed through selected statistical tool while using mean difference and 95% confidence interval for analysis. Results: Mild to moderate physical activity in the form of exercise or physiotherapy intervention for 4 to 24 weeks improved the scores of QoL in post-CABG patients in intervention group (36.41 ±5.12) as compared to control group (25.39 ±6.22) at the end of follow up period. In comparison to control group, scores of both physical and mental components including psychological role, physical function, general health, bodily pain, social function, vitality, and mental health were improved for post-CABG patients in intervention group. Conclusion: Results reveal that mild to moderate physical activity can improve quality of in post-GABG patients moderate manner, however, further investigation in large population size in needed prior to recommending physical activity in rehabilitation programs of the target patients.
MORPHOMETRIC STUDY OF Kalophrynus palmatissimus AT TWO FOREST RESERVES: AYER HITAM FO...
Muhammad Abdul Aziz
Marina Mohd. Top @ Mohd. Tah

Muhammad Abdul Aziz

and 4 more

May 18, 2020
A research study on morphometrics of Kalophrynus palmatissimus (known as Lowland Grainy Frog) at Ayer Hitam Forest Reserve (AHFR), Selangor and Pasoh Forest Reserve (PFR), Negeri Sembilan was carried out from 12 November 2016 to 13 September 2017. The study was conducted to examine data on the morphometric traits of K. palmatissimus at the two forest reserves. 15 morphometric traits of K. palmatissimus were taken by using vernier calipers. Frog surveys were done by using 15 and 18 nocturnal 400 m transect lines at AHFR and PFR, respectively. In addition, five climatic data were recorded. The results showed that most of the morphometric traits in AHFR (n = 34) and PFR (n = 31) were positively correlated within each other. General Linear Model (GLM) analysis, showed that snout-vent length (SVL) influenced most morphometric traits, except for hand length. Later, it was found that the snout-vent length of K. palmatissimus in AHFR were slightly larger than PFR. From PCA analysis, morphometric traits were grouped into two components for AHFR and PFR, respectively. In AHFR, head length, eye diameter, head width, internarial distance, interorbital distance, forearm length, tibia length, foot length, and thigh length were strongly correlated while snout length and eye-nostril distance were strongly correlated. In PFR, eye diameter, head width, internarial distance, interorbital distance, foot length and thigh length were strongly correlated, while snout length and eye-nostril distance were strongly correlated; hence, suggesting that all morphometric traits grow simultaneously in K. palmatissimus with eye-nostril distance (EN), and snout length (SL) were closely growing simultaneously at AHFR and PFR. To conclude, the data collections showed the 15 different morphometric traits of K. palmatisssimus between AHFR and PFR with K. palmatissimus at AHFR were slightly larger than at PFR. Key words: Kalophrynus palmatissimus, forest reserve, morphometrics, climatic factors, transect lines
Thermodynamic analysis of an ecologically restored plant community:Theoretical basis
Mingli Chen
Zijian Wu

Mingli Chen

and 4 more

May 18, 2020
The nature of matter and energy exchange of an ecological process defines the applicability of the thermodynamic functions for describing an ecosystem. A plant community is an open system consisting of living species as material components. Following the basic laws of thermodynamics, the enthalpy H stored in biomass form of a plant community will be related to its total equivalent biomass quantity CT, the weighted average standard chemical potential μ0, Gibbs free energy G, entropy S and temperature T by H = G + TS = CTμ0. Using h, f and s to denote H/(RT), G/(RT) and S/R (R denoting the gas constant), respectively, the conventional function can be transformed to h = f + s = CTμ0/(RT). The relation sm/CT = SIm = ln(N) derived from the maximal discrete entropy theorem shows that sm (the maximum s) and SIm (the maximum information entropy) will increase with increase in the total number of species N, suggesting that N has an upper limit Nm subject to regional species resource. As an upper limt of SI and s/CT, ln(N) is applied as a biodiversity index. As an upper limt of ln(N), ln(Nm) can thus be regarded as a biodiversity potential index as it takes into account the available number of species distributed in the surrounding areas of the plant community, showing the potential limit for further increase in its biodiversity. The difference between ln(Nm) and ln(N) dtermines the distribution of H as G and TS, indicating that the internal energy distribution of an acosystem is a function of its productivity and biodiversity. The potential trends of increasing N towards Nm and increasing s towards sm suggest that an ecosystem can possess natural trends towards increase in both its species richness and evenness.
pysisyphus - Exploring Potential Energy Surfaces in Ground- and Excited States
Johannes Steinmetzer
Stephan Kupfer

Johannes Steinmetzer

and 2 more

May 18, 2020
Predicting the energetics of chemical transformations requires localizing stationary points on a potential energy surface. Whereas educts and products of a chemical reaction may be known, transition state optimization is challenging, as good guesses may be unavailable. Extending stationary point searches to excited states leads to additional difficulties as several states may be close in energy, requiring efficient state-tracking. Herein we report the implementation of pysisyphus, an external optimizer, that allows not only the localization of stationary points in the ground state, but also for excited states by providing several state-tracking algorithms. Pysisyphus offers all necessary tools for calculating reaction paths starting from the optimization of the reactants, running chain-of-states methods like the nudged elastic band or the growing string method with subsequent transition state optimization and a concluding intrinsic reaction coordinate calculation.
← Previous 1 2 … 2585 2586 2587 2588 2589 2590 2591 2592 2593 … 2754 2755 Next →

| Powered by Authorea.com

  • Home