To address the insufficient configuration of real-time measurement devices in distribution networks, a state estimation method for distribution networks based on a CNN-BiLSTM network and a customised HGGA algorithm is proposed. First, the historical data and real-time data are used as the input of the CNN-BiLSTM network to obtain the branch power pseudo measurement and load node injected power pseudo measurement with high accuracy at the current moment. Second, the pseudo measurement set of the input state estimation is determined using the customised HGGA algorithm. Finally, the real-time measurement, pseudo measurement, virtual measurement, and weight set are input into the state estimation program to complete the real-time state estimation of the distribution network based on the weighted least squares method. Additionally, the proposed method is applied to the data fusion of different measurement systems. Theoretical analysis and example verification confirm that the proposed method can effectively improve the accuracy of pseudo measurement modelling and state estimation results.

In order to explain the polarization Raman noncoincidence effect of specific polar bond，and the noncoincidence phenomenon between FT-Raman and FT-IR spectra, the aggregation induced spectral splitting theory were proposed. In this paper, the vibration splitting theory was demonstrated by two strategies, improving the spectral resolution by cryogenic matrix isolation technique or finding cases where the coupling splitting is large enough to be distinguished. The band of monomer and dimer splitting were detected for acetone cryogenically isolated by Ar matrix. The polarization Raman spectra of β-propiolactone (PIL)/CCl4 binary mixture were collected at room temperature, the spectral splitting phenomenon can be clearly observed. The dynamic transformation between the monomer and dimer can be achieved and detected clearly by simply adjusting the PIL concentration. The theoretical DFT calculation based on the monomer and dimer of PIL, as well as the FT-IR and FT-Raman spectra of PIL confirmed further the observed splitting phenomenon.

Little is known about longitudinal health risks and justice involvement among juvenile justice system (JJS) populations. This study used a sample of n = 388 males involved in the JJS to examined longitudinal associations of a latent health risk factor, comprised of depression, marijuana use, and sexually transmitted infections, with post-JJS placement. Results indicate the health risk factor was relatively stable over three time points of JJS entry and associated with present and future JJS placement. Youths who were Hispanic, older, or living with a family member struggling with alcohol use had higher health risk. These findings underscore the need for JJS services that address health risks among persistent juvenile offenders, with consideration of cultural and family dynamics.

Barley is the most produced crop in Ireland; it is essential as a fodder crop and a key ingredient for the malting industry, contributing to Irish national identity. In Ireland, climate change is bringing more extreme rain; leading to an increase in flooding and waterlogging events. Barley is particularly sensitive to waterlogging urging the need to harness genetic diversity and breed barley with increased waterlogging tolerance to maintain current agricultural production. One of the limiting factors in breeding is the drawbacks of traditional phenotyping. This project relies on modern high-throughput phenotyping, which allow non-destructive, continuous and quantitative data collection. Using imaging sensors in controlled conditions, we phenotyped a collection of barley accessions under controlled and waterlogged conditions using RGB, fluorescent and hyperspectral cameras (VNIR and SWIR). We used the core European Barley Heritage collection (ExHIBiT); made up of 230 diverse 2-row spring barley accessions. This collection was assembled, genotyped, agronomically characterized and its application for association mapping has been established by our team. We observed that 14 days of waterlogging lead to a significant reduction in pixel count (Project Shoot Area) and Quantum yield, showing a large impact on several hyperspectral indices. We also observed that 7 days of recovery after stress are fundamental for differentiate stress resilience. Work is ongoing to optimize hyperspectral image analysis, and establish parameters to distinguish plant performance, enabling to discriminate between resilient and sensitive accessions. These data will be used for association mapping to identify genetic regions contributing to waterlogging tolerance in spring barley.

Setting-up a high quality and efficient pharmacovigilance (PV) system in multi-country clinical trials can be more challenging for academic sponsors than for industrials. Generating high-quality safety data, compliant with legal and regulatory standards such as European or World Health Organization (WHO), places special requirements on the PV system. The scenario becomes more complicated when dealing with multi-country European clinical trials where heterogeneity on safety process can be encountered. Some Sponsors face these challenges are even more difficult for pediatric trials. A possible solution to ensure that the safety of all participants is equally guaranteed and the PV system fulfills all regulations could be to set up a centralized PV system. This paper introduces the key points to consider when implementing and organizing such system in multi-national European clinical trials. It is based on the Inserm-ANRS MIE pharmacovigilance department’s experience and aims to harmonize and anticipate the needs, in particular by implementing safety procedures and a network of local safety officers. This system is very useful to respond to the challenges of a European clinical trial, notably when considering the complexity of local safety requirements of each country, signal management and the specificities of paediatric regulation.

Introduction: Silver nanoparticles have been extensively investigated in dental and orthopedic materials. However, the impregnation of bone graft with silver nanoparticles has been poorly investigated. Objectives: The aim of this study was to evaluate the in vitro antibiofilm activity of a bovine bone impregnated with silver nanoparticles. Methods: Bone scaffolds from cancellous bovine femur were used for the tests and impregnated with silver nanoparticles (50nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentration were performed Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Acinetobacter baumannii, and Escherichia coli. Disc diffusion tests for silver nanoparticles susceptibility and quantification of biofilm production on plate and bone with sessile cell count were also performed. Results: All pathogens were susceptible to silver with low minimal inhibitory concentration (0.25 – 4 mg/L). The scaffold impregnated with silver nanoparticles presented a significant reduction in the biofilm cells for all microorganisms with a reduction of more than 3 logs in colony forming units count. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce biofilm, and it can be a strategical material to be used as an implant for different approaches.

Background: Ventilator-associated pneumonia (VAP) is one of the most common causes of nosocomial infections and is associated with prolonged hospitalization, increased health care costs, and high mortality of critically ill patients during hospitalization in intensive care units (ICUs). Objective: To characterize and evaluate in vitro and in vivo antimicrobial and anti-biofilm activity of an in-house tracheostomy tube impregnated with chlorhexidine and violet crystal. Methods: The tracheostomy tubes were tested in vitro for their ability to prevent biofilm formation by standard strains of S. aureus, P. aeruginosa, and E. coli, and multidrug-resistant bacteria obtained from clinical cultures: Meticillin-resistant S. aureus (MRSA), and carbapenem-resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae. Results: The impregnated tracheostomy tubes demonstrated antimicrobial activity, including for multidrug-resistant bacteria. In this pilot study, 14 patients were evaluated, seven in the chlorhexidine and violet crystal-coated group and seven in the control group. During ventilation, VAP occurred in one patient in the coated group and in three patients in the control group (p=0.28). The biomass in the impregnated tubes did not differ from the control group and no difference was found in the production of sessile cells by the quantitative method, with a median of 15.50 cfu/mL (IQR25-75% 12.00-196.50) and 168.00 cfu/mL ((IQR25-75% 78.50-250.00), respectively. Conclusion: This study provides preliminary evidence to support that antiseptic impregnation of tracheostomy tube provides significant antimicrobial activity.

Atrial septal pouch is a common anatomic variant of the interatrial septum and a nidus for thrombus formation. Left-sided atrial septal pouch has been associated with an increased risk of cryptogenic stroke. We described a case of left-sided atrial septal pouch thrombus, incidentally found prior to transseptal puncture during Watchman device placement. This case highlights the importance of a thorough echocardiographic evaluation of the atrial septum prior to transseptal access and recognition of this finding as a potential cardiac source of embolism.

Understanding the temporal stability of spatial patterns of soil moisture (SMSP) is essential for predicting and modelling soil moisture; however, there is a relative lack of existing research on the effects of long-term vegetation restoration on the temporal stability of soil moisture. In this study, the temporal stability of soil moisture in the catchment at different stages of vegetation restoration (10 years (RF10), 20 years (RF20) and 40 years (RF40) of farmland rehabilitation) and the associated influencing factors were investigated. The results show that within the RF10 catchment, water deficits occurred during the growing season and that long-term revegetation improved soil moisture in the catchment and increased the water surplus. The overall spatial pattern of soil moisture was consistent across the three catchments. Decreases in soil moisture stability indicators (MRD and ITS) and increases in Spearman’s rank correlation coefficients for soil moisture suggest that revegetation has enhanced the stability of the spatial pattern of soil moisture in the catchment. In addition, the R2 fit of moisture at representative sample sites to the mean moisture in the catchment increased; fluctuations in the spatial pattern of soil moisture were mainly influenced by plant indicators such as fine root biomass (FRB), shrub grass biomass (SGB) and leaf area index (LAI) in the first and middle stages of revegetation, while the temporal stability of soil moisture gradually became more dependent on soil indicators such as Clay and SOC in the middle and late stages of revegetation.

Many proteins must interact with molecular chaperones to achieve their native folded state in the cell. Yet, how chaperone binding and binding-site characteristics affect the folding process is poorly understood. The ubiquitous Hsp70 chaperone system prevents client-protein aggregation by holding unfolded conformations or by unfolding misfolded states. Hsp70 binding sites of client proteins comprise a nonpolar core surrounded by positively charged residues. However, a detailed analysis of Hsp70 binding sites on a proteome-wide scale is still lacking. Further, it is not known whether proteins undergo some degree of folding while chaperone bound. Here, we begin to address the above questions by identifying Hsp70 binding sites in 2,258 E. coli proteins. We find that most proteins bear at least one Hsp70 binding site and that the number of Hsp70 binding sites is directly proportional to protein size. Aggregation propensity upon release from the ribosome correlates with number of Hsp70 binding sites only in the case of large proteins. Interestingly, Hsp70 binding sites are more solvent-exposed than other nonpolar sites, in protein native states. Our findings show that the majority of E. coli proteins are systematically enabled to interact with Hsp70 even if this interaction only takes place during a fraction of the protein lifetime. In addition, our data suggest that some conformational sampling may take place within Hsp70-bound states, due to the solvent exposure of some chaperone binding sites in native proteins. In all, we propose that Hsp70-chaperone-binding traits have evolved to favor Hsp70-assisted protein folding devoid of aggregation.

Landscape genetics is increasingly transitioning away from microsatellites, with Single Nucleotide Polymorphisms (SNPs) providing increased resolution for detecting patterns of spatial-genetic structure. This is particularly pertinent for research in arid-zone mammals due to challenges associated with unique life history traits, boom-bust population dynamics and long-distance dispersal capacities. Here, we provide a case study assessing the performance of SNPs versus microsatellites in evaluating three explicit landscape genetic hypotheses (isolation-by-distance, isolation-by-barrier, and isolation-by-resistance) in a suite of small, arid-zone mammals in the Pilbara region of Western Australia. Using clustering algorithms, Mantel tests, and linear mixed effects models, we compare functional connectivity between genetic marker types and across species, including one marsupial, Ningaui timealeyi, and two native rodents, Pseudomys chapmani and P. hermannsburgensis. SNPs resolved subtle genetic structuring not detected by microsatellites, particularly for N. timealeyi where two genetic clusters were identified. Furthermore, stronger signatures of isolation-by-distance and isolation-by-resistance were detected when using SNPs, and model selection based on SNPs tended to identify more complex resistance surfaces (i.e., composite surfaces of multiple environmental layers) in the best-performing models. While we found limited evidence for physical barriers to dispersal across the Pilbara for all species, we found that topography, substrate, and soil moisture were the main environmental drivers shaping functional connectivity. Our study demonstrates that new analytical and genetic tools can provide novel ecological insights into arid landscapes, with potential application to conservation management through identifying dispersal corridors to mediate the impacts of ongoing habitat fragmentation in the region.

This report describes the evisceration of the jejunum following acute dehiscence of the abdominal wall in an 18 year old gelding which had undergone treatment for suspected sarcoids on the ventral abdomen with a topical caustic/chemotherapy agent 6 weeks previously. The sarcoid lesions appeared to be responding appropriately to the topical therapy until the day of presentation. The jejunum was safely contained in an abdominal bandage and the horse underwent emergency referral before immediate anaesthesia and further assessment. The horse was euthanased at the owners’ request following a guarded prognosis.

In order to accurately and quickly predict the storage life of non-asbestos gaskets, on the basis of accelerated aging tests in a hot air environment, the acceleration coefficients at different accelerated temperatures were calculated to simulate the storage aging process of the gaskets. According to the relationship between performance change of the gasket and aging time, used the Arrhenius model and taking compressibility as the performance index, obtained the relationship between the compressive performance change and storage time of sample 1 (with multi-walled carbon nanotubes (MWCNTs)) and sample 2 (without MWCNTs added). The results show that the compressive properties of the non-asbestos gaskets gradually deteriorated with the prolongation of the aging time or the increase of aging temperature. An aging life model under a storage environment was established to evaluate the storage life of non-asbestos gaskets at 25 °C. The extrapolation results show that the storage life of the gasket with MWCNTs was 2.91 years and 1.81 years for the storage life of the gasket without MWCNTs at 25 ℃. Thus, adding MWCNTs can effectively improve the storage life of non-asbestos gaskets.

Fatigue results from the occurrence of several damage mechanisms and their interactions. The cyclic plastic strain and damage accumulation at the crack tip are widely pointed as the main agents behind FCG. In this work, the authors propose the prediction of FCG through a node release numerical model that offers several possibilities regarding the modelling of the mechanisms behind fatigue. A hybrid propagation method is presented where both cumulative plastic strain and porous damage represent parallel propagation criteria. Accordingly, the node is released once either a critical plastic strain or a critical porosity, at the crack tip, is reached. The Gurson-Tvergaard-Needleman (GTN) damage model is employed to predict porous damage evolution through the processes of nucleation and growth of microvoids. The model is validated through comparison with experimental data. Finally, the interactions between plastic strain, porous damage, crack closure and stress triaxiality are accessed.

Day to day life has become changed after the wide-spreading adaptability of AI Researchers and AI scientists are making their unstinting efforts to make human life more comfortable and technologically advanced by using AI tools and techniques. Sustainable education is an approach of aiming to establish the sustainability act in students, and communicate the values among schools, colleges and communities the values and motivations future-in one's own life, in their communities, and on a worldwide platform.Along with I.C.T. students, their fresher professionals also need an intelligent system that can understand each professional's learning needs and help them excel in their skills.

We reported a case of a 53-year-old patient with coarctation of aorta and multiple aneurysmatic changes on aortic arch. Enhanced CT and reconstruction revealed significant coarctation and multiple aneurysmatic dilatation. The patient underwent stent implantation and was discharged with symptoms relived. Follow-up examination progression of aneurysms, however, without symptoms.

Background. Respiratory syncytial virus (RSV) infection is a cause of substantial morbidity and mortality in young children. There is currently no effective therapy available. Methods. This was a phase 2 study of the oral RSV fusion protein inhibitor AK0529 in infants aged 1-24 months, hospitalized with RSV infection. In part 1 patients (n=24) were randomized 2:1 to receive a single dose of AK0529 up to 4 mg/kg or placebo. In part 2 patients (n=48) were randomized 2:1 to receive AK0529 at 0.5, 1 or 2 mg/kg bid or placebo for five days. Sparse pharmacokinetic samples were assessed using population pharmacokinetics modelling. Safety, tolerability, viral load, signs and symptoms were assessed daily during treatment. Results. No safety or tolerability signals were detected for AK0529: grade ≥3 treatment-emergent adverse events occurred in 4.1% of patients in AK0529 and 4.2% in placebo groups, respectively, and none led to death or withdrawal from the study. In part 2, targeted drug exposure was reached with 2 mg/kg bid. A numerically greater reduction in median viral load with 2 mg/kg bid AK0529 than with placebo at 96 hours was observed. A -4.0 (95% CI: -4.51, -2.03) median reduction in RSV signs and symptom score from baseline to 96 hours was observed in the 2 mg/kg group, compared with -2.0 (95% CI: -3.42, -1.82) in the placebo group. Conclusions. AK0529 was well tolerated in hospitalized RSV-infected infants. Treatment with AK0529 2 mg/kg bid was observed to reduce viral load and clinical signs and symptoms.

Background. Patients are allowed to be released from quarantine after virus turns negative for asymptomatic or mild covid-19. Factors affecting the negative conversion of nucleic acid are unknown. Methods. This study included asymptomatic or mild covid-19 patients quarantined in Shanghai shelter hospital from April to May 2022. They were tested daily for SARS-CoV-2 nucleic acid. The clinical characteristics were collected. Univariate and multivariate Cox regression were used to explore the relationship between clinical characteristics and the time of virus turning negative (TTN), finally a risk model was established. Results. A total of 7836 patients enrolled were divided into training or validation set randomly. In training set, age (≥40 Y), symptoms (yes) , diabetes (yes) and vaccination status(yes) were correlated with TTN. We used HR values of the above factors in multivariate Cox regression as risk coefficients to build a risk model for predicting TTN. TTN of patients with high risk score was longer than with low risk score. This risk model has been well validated in the validation set and the complete set. Conclusions. Our study found that age, symptoms, diabetes and vaccination status were correlated with the TTN,and we first build a risk model to predict TTN in asymptomatic or mild Covid-19.

Introduction Post ablation of the accessory pathway (AP), the patient is observed in the catheterization laboratory for a variable period for resumption of pathway conduction. Aim of the study was to determine whether the administration of intravenous adenosine at 10 minutes after ablation of accessory pathway (AP) would have the same diagnostic accuracy as waiting for 30 minutes in predicting the resumption of AP conduction. Methods: This was a prospective interventional study conducted in two centers. Post ablation of the AP, intravenous adenosine was administered at 10 minutes to look for dormant pathway conduction. The response was recorded as positive (presence of pathway conduction), negative (absence), or indeterminate (not able to demonstrate AV and VA block and inability to ascertain AP conduction). Results: The study included 110 procedures performed in 109 patients. Adenosine administration at 10 minutes showed positive result in 3 cases (2.7%), negative result in 99 cases (90%) and indeterminate result in 8 cases (7.3%). Reconnection of accessory pathway at 30 minutes post ablation was seen in 8 cases (7.3%). Of these 8 cases, 10minutes adenosine administration showed positive test in 3 patients and negative test in 5 patients. Adenosine test at 10 minutes has a sensitivity, specificity, positive predictive value, and negative predictive value of 37.5%, 100%, 100% and 94.9% in identifying the recurrence of accessory pathway conduction at 30 minutes, respectively. Conclusion: Absence of pathway conduction on administration of adenosine 10 minutes post ablation does not help predict the absence of resumption of conduction thereafter.

Background Despite several targeted antiviral drugs against SARS-CoV-2 currently being available, the application of type I interferons (IFNs) still deserves attention as an alternative antiviral strategy. This study aimed to assess the therapeutic effectiveness of IFN-α in hospitalized patients with COVID-19 and community-acquired pneumonia of viral etiology. Methods The prospective cohort study included 130 adult patients with coronavirus disease (COVID-19). 80,000 IU of IFN-α2b was administered daily intranasally for 10 days. The primary parameters were the length of hospital stay, CT-diagnosed lung injuries and the SpO2 level after-before dynamics following IFN-α2b treatment. Results Adding IFN-α2b to standard therapy for patients with severe COVID-19 reduces the length of the hospital stay by 3 days (p < 0.001). The level of CT-diagnosed lung injuries was also reduced from 35% to 15% (p = 0.011) at the time of discharge, and CT injuries decreases at the end of hospital stay from 50% up to 15% (p = 0.017). In the group of patients receiving IFN-α2b, the SpO2 index before and after treatment increased from 94 (92-96, Q₁ - Q₃) to 96 (96-98, Q₁ - Q₃) (p < 0.001), while the percentage of patients with normal saturation increased (from 33.9% to 74.6%, p < 0.05), and decreased in the categories low (from 52.5% to 16.9%) and very low (from 13.6% to 8.5%). Conclusion Addition of IFN-α2b to standard therapy has a positive effect on the course of severe COVID-19. Nevertheless, more research on the therapeutic use of Type I IFNs should be performed.

High sensitivity flexible strain sensors that can perceive and respond to strain stimulation are an essential part of wearable smart devices. Yet, the detection of subtle strains on the human body and external stimulation suffers from low sensitivity and slow response of many existing sensors. Here, an ultrasensitive flexible optical sensor based on microfiber coupler encapsulated by Polydimethylsiloxane (PDMS) has been proposed to realize weak strain detection and fast response. Benefiting from dramatic change of coupling ratio (CR) of the microfiber coupler under weak strain, this flexible strain sensor exhibits ultrahigh strain sensitivity (gauge factor, GF=900), low detection limit of 0.001%, ultrafast response time (< 0.167 ms), and superior durability and stability (>10 000 cycles). Synchronously, the simultaneous detection of CR and loss parameters not only realizes the 0.001% low detection limit, but also expands the sensing range to 0.45%. This highly sensitive microfiber coupler sensor has been demonstrated to capture and recognize respiration, broadband sound signals and pulse waves at different sites of human body in real time. Moreover, based on frequency division multiplexing technology, the wrist pulse and human voice could be detected and demodulated simultaneously. This flexible photonics strain sensor could be useful in the development of convenient and wearable healthcare devices. Corresponding author(s) Email:  msgan@scut.edu.cn； zhangzs@fosu.edu.cn;

Adeyemi O. Omoge1*; Modupe O. Oyedele2; Oluwabunmi B. Erinsakin3& Oladunni D. Oluwafemi4

The preprocedural imaging before the left atrial appendage closure device is critical for selecting the appropriate device for the appropriate patient. CT scans and TEE have been used in the past for preprocedural imaging. In this report, the authors used cardiac MRI as a pre procedural planning tool in the wake of scarcity of contrast availability.

Tomato chlorosis virus (ToCV) is a member of the genus Crinivirus in the family Closteroviridae. It has a wide host range and wide distribution, causing serious harm to the vegetable industry. The autophagy pathway plays an important role in plant resistance to virus infection. Viruses and plant hosts coevolve in defence and antidefence processes around autophagy. In this study, ToCV p22 protein was selected to verify the interaction between ToCV p22 and NbBAG5. Through overexpression and downregulation of NbBAG5, it was found that NbBAG5 could negatively regulate ToCV infection. NbBAG5 was found to be localised in mitochondria and can change the original localisation of ToCV p22, which is colocalised in mitochondria. Further study found that NbBAG5 inhibited the expression of mitophagy-related genes and the number of autophagosomes, thereby regulating viral infection by affecting mitophagy. In summary, this study demonstrated that ToCV p22 affects autophagy by interacting with NbBAG5, established the association between virus infection, BAG proteins family and autophagy pathway and explained the molecular mechanism by which ToCV p22 interacts with NbBAG5 to inhibit autophagy to regulate viral infection.