Pancreatic cancer is the third most common cause of cancer-associated deaths across the world. Modern cancer treatment such as chemotherapy has several side effects including tissue damage. Therefore, there is a dire need to evaluate the natural substances having anti-carcinogenic activities without any negative or side impact on human health. The current study aimed to investigate the in-vitro and in-vivo anti-carcinogenic potential of ginger ( Zingiber Officinale). For in-vitro analysis, the ginger extract was prepared and antioxidant activities were accessed by the DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay (DPPH). The anti-proliferative activities were measured using 3[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Human pancreatic cancer (Panc-01 cells) was treated with aqueous ginger extract (GE) at different concentrations (0, 12.5, 25, 50,100, and 200µg/ml) for both 24 and 48 hours. For the in-vivo experiment, a total of 30 albino rats aged 7-8 weeks and weighing 115-130 grams were randomized into five groups (n=6 rats/group) and groups were allotted to one of two GE dosage levels (50 and 80 mg) by following the completely randomized design. The GE was given orally during the experimental duration (30 days). The results demonstrated that GE significantly decreased the tumor mass volume bearing significant antioxidant and antiproliferative potential. IC 50 values for 24 and 48 hours of treatment were found to be 0.73 µg/ml and 0.76 µg/ml on the Panc-01 cell line. The present study suggested that GE has immense potential to inhibit tumor progression without affecting the normal physiology and functioning of the body and thus can be used as a cancer therapeutic agent.