Antimicrobial resistance (AMR) poses a growing global threat, compromising the effective treatment of infections and increasing morbidity and mortality rates. This study investigated the antibacterial potential of bacteria associated with termites as an alternative therapy. Thirty-three termites were processed for bacterial isolation and identification using biochemical tests, BD Phoenix, and 16S rRNA sequencing. Secondary metabolites from isolates were extracted with ethyl acetate and evaluated for antibacterial activity via agar diffusion methods. GC-MS identified bioactive compounds, Insilco technique was performed via STRING analysis. PCR targeting the 16S rRNA gene produced 1.0 kb DNA fragments. Sequencing identified all as Bacillus cereus (99.06–99.77% similarity; Accession Nos. PV660301–PV660305) with homology to GQ406846.1, MT103054.1, and OP830493.1. Antibacterial activity was observed against E. coli (12–15 mm), S. aureus (17–21 mm), Citrobacter sp. (13–15 mm), and S. typhi (12–15 mm), with T2 and T3 showing highest activity against S. aureus (21 mm) and Citrobacter sp . (15 mm), respectively. Ciprofloxacin (control) yielded 16, 24, 17, and 22 mm zones. PV660301–PV660305 produced 17–23 metabolites each, with major compounds including 1-Heptacosanol (53.17%), Squalene (32.45%), Dibutyl phthalate (up to 24.24%), and a Vitamin E derivative (14.77%). STRING network analysis of squalene in S. aureus identified 11 nodes and 55 edges, enriched in steroid biosynthesis (hsa00100), KEGG metabolic pathways (hsa01100, hsa00900), and sterol-related functions, highlighting squalene’s role in membrane integrity and virulence. Termite-associated Bacillus cereus showed strong antibacterial potential, warranting further elucidation of the metabolite via NMR