Objectives: Present study tested the distinctive mediation model of moral injury (MI) and positive affect with burnout via wellbeing and negative affect. It also examined the moderating effect of positive emotions in the association between MI with wellbeing and negative affect among police officers. Furthermore, this study provided data about the prevalence of MI and burnout among a sample of police officers. Methods: One hundred eighty-seven active law enforcement officers completed the online self-report measures. The hypothesized model included MI as the predictor, positive affect as moderator, wellbeing and negative affect as mediators, burnout as the outcome, and gender as covariate. Results: The indirect effects of MI on burnout via wellbeing ( ab = 0.08) and negative affect ( ab = 0.08) were significant. Similarly, the indirect effect of positive affect on burnout via well-being ( ab = –0.12) was significant. The indirect effect of positive affect on burnout via negative affect was insignificant ( ab = –0.04). Positive affect did not moderate the association between MI and well-being ( ab = 0.12) or negative affect ( ab = 0.04). Officers showed low to high scores on various subscales of MI and burnout. Conclusion: The results highlighted the importance of considering MI and positive affect as phenomena that can impact law enforcement officers’ well-being, emotional state, and burnout.