Objective: It was aimed to analyze the speech-FFR of Turkish-speaking young adults and middle-aged adults with normal hearing thresholds and to compare the values according to age and gender. Methods: Thirty-one young adults with normal hearing thresholds between the ages of 19 to 25 years and thirty healthy middle-aged adults between the ages of 40 to 57 years participated in this study. The 40 ms speech syllable /da/ was used to record speech-FFR waveforms with Intelligent Hearing Systems at 80 dB SPL from each participant’s right ear. Results: In young adults, females generally exhibited shorter latencies than males in all peaks, while middle-aged females had shorter latencies in A and D peaks compared to males. Young females showed shorter latencies in most peaks compared to middle-aged females, except for the A peak. Young adult males had shorter D and O peak latencies compared to middle-aged males. Amplitude differences were observed only in the V peak for age, with no significant gender-related differences in any peak amplitudes. Overall, there were no significant differences between groups and subgroups in spectral peak amplitudes concerning age and gender. Interpretation: The study revealed the effect of gender and age on neural processing of speech sounds in the brainstem. Although the older participants were in the middle age group, the study revealed that aging began to disrupt the coding of speech and integration in the brainstem. Speech-FFR latency, amplitude and spectral characteristics were determined for young and middle-aged Turkish-speaking individuals.