This study investigates the pollution factors present in the water of the Al-Shatrah Stream, focusing on identifying the health risks associated with these factors. To achieve this objective, the researcher employed the Canadian Water Quality Index (CWQI) as a tool for classifying and examining the impact of spatial and seasonal variations on pollution levels. The study analyzed nine indicators, including physical and chemical parameters such as temperature, turbidity, pH, total dissolved solids, total suspended solids, chlorides, sulfates, and alkalinity. Additionally, a bacteriological analysis was conducted to assess the presence of fecal coliform bacteria at three sites along the stream during the months of February and July. The researcher employed statistical analysis to calculate the arithmetic mean and standard deviation of the measured values, both temporally and spatially. Variations between sites were assessed using analysis of variance (ANOVA), and the least significant difference test was applied at a significance level of 0.05 for all indicators. The findings from the Canadian Water Quality Index (CWQI) analysis are concerning, as they indicate that the water quality of the Al-Shatrah Stream is subpar and unsuitable for human consumption. The CWQI equation, a widely accepted and reliable method for assessing water quality, has classified the sampled sites along the stream as having poor water quality.