Background Equine Herpes Myeloencephalopathy (EHM) is a rare manifestation of Equine Herpesvirus-1 (EHV-1) infection. EHM outbreaks have been documented in Europe, North America, and New Zealand, no confirmed cases have been reported in India. Objectives The study presents the virological, clinic-pathological, and outcome data from two EHM outbreaks in Punjab, India. Study design Descriptive epidemiology Methods Two outbreaks of Equine Herpes Myeloencephalopathy (EHM) were reported from distinct agro-climatic zones of Punjab, India. This study documents the clinical, serological, molecular, and histopathological findings of the reported outbreaks. Blood and serum samples were used for virus neutralization test (VNT) to assess serological responses, and quantitative PCR (qPCR) for molecular detection of EHV-1. Additionally, nasal swabs were collected for virus isolation, and tissue samples from necropsied horses were tested for both virus isolation and qPCR. Relative quantification of EHV-1 DNA was performed using real-time PCR targeting gB gene (ORF33) Results In the first outbreak, three stallions showed variable clinical signs, including facial nerve paralysis, hind limb paresis, prolapsed penis, fever, hind limb swelling, and episodic seizures. The outbreak was confirmed serologically, with VNT titers greater than 1:4. The second outbreak began with a 7-year-old stallion showing fever, ataxia, and paresis, which collapsed. EHV-1 was detected in nasal and ocular swabs, and tissue samples of the dead animal. qPCR amplification using specific primers for the EHV-1 gB gene confirmed EHV-1 infection, distinguishing it from EHV-4. No other horses on the farm showed clinical signs of EHM, but one of four horses, a 6-year-old stallion, tested positive for EHV-1 with a VNT titer of 1:8. Main Limitation Small sample size Conclusion This study describes two sporadic outbreaks of Equine Herpesvirus Myeloencephalopathy (EHM) in India, which were limited to a small number of horses. Serological, molecular, virus isolation, and histopathological analyses confirmed the presence of EHV-1.