As a globally strategic fiber crop, cotton (Gossypium hirsutum L.) faces escalating yield constraints from abiotic stresses, particularly drought [(Zhu, 2016)](#ref-0001). Therefore, finding genes that increase cotton drought tolerance, while cultivating genetically modified cotton with enhanced drought tolerance, is currently one of the important breeding goals for breeders [(Genc, et al., 2019)](#ref-0002). This study functionally characterizes an R2R3-MYB transcriptional regulator, GhMYB4, that orchestrates molecular responses to water-deficit conditions.