Celery root, derived from the Apium graveolens plant, represents a nutritionally potent vegetable. Celery roots have garnered attention from the scientific community not only for antioxidant and anti-inflammatory effects but also for the potential utilization of celery root-plant-derived vesicles (CR-PDVs) through a biotechnological approach. This study delves into the recent surge of research on the biochemical composition of the vesicles originating from celery roots, revealing promising prospects for various diseases. The investigation focuses on the elucidation of vesicle content derived from celery root lysate, aiming to understand its potential applications. The aim is to provide insights into potential future applications by isolating and conducting physical and biochemical analyses of plant vesicles obtained from celery roots. After the isolation of CR-PDVs, size distribution, and concentration determination was performed for physical characterization. Subsequently, proteomic analysis of the isolated vesicles, including detailed database analyses, was carried out. The results highlight the multifaceted roles of proteins within CR-PDVs, encompassing functions such as folding, biogenesis, and carrier systems. Considering the observed content of CR-PDVs, this study opens avenues for their potential utilization in various disease models, including neurodegenerative and autoimmune diseases. The comprehensive characterization of CR-PDVs offers valuable insights for their application in therapeutic development.