The structural strength of the levitation frame is of critical importance for the safe operation of medium and low-speed maglev vehicles. However, the diverse load conditions in the engineering application environment and the current lack of relevant test and evaluation standards pose greater challenges to its reliability and safety. Accordingly, this paper combined classical numerical and dynamic modeling to obtain load boundaries. Subsequently, a full-factor finite element model was established and a full-scale test bench was built. The fourth strength theory and the modified Goodman-Smith fatigue limit diagram are used to systematically evaluate the structural strength and mark the weak points. Finally, a fatigue test with enhanced cyclic loading of 1×10 7 sinusoidal alternating loads is carried out, and the DPT-5 penetrant inspection agent is used for overall flaw detection. The research results show that: (1) the structural strength of the lightweight-designed levitation frame meets the evaluation requirements; (2) the weak points mostly appear at the welds, rib plates and corners of the longitudinal beams, arms and anti-rolling beams under multi-directional loads; (3) no fatigue cracks are found in the overall structure and each weak point after each stage of the tests by penetrant inspection.