Background: Oral squamous cell carcinoma (OSCC) is one of the most common oral malignancies. Since the prognosis is directly related to it’s stage, timely diagnosis is important. miRNAs are potential biomarkers that can regulate the process of carcinogenesis. By examining their expression, it may be possible to use them for diagnosis . Therefore, this study aimed to investigate the expression level of miRNA-376a,b in OSCC and normal adjacent tissue. Materials and methods: 30 tissue samples of OSCC were obtained. The expression level of miR-376a,b was measured using qRT-PCR technique. To compare the expression levels of miR-376a,b, the Paired Sample T Test was used. Also, multiple linear regression test was used to investigate the relationship between age, sex, tumor size, stage and grade with the marker expression level. Result: The results revealed that the average expression level of miR-376a,b in the tumor group was significantly higher(p=0.000). There was no significant correlation between the patients’ age and sex or tumor’s size, stage and grade and with the expression of these markers(p>0.05). Conclusion: The notable increase in the expression of miR-376a,b in OSCC samples suggests that these miRNAs may have potential as biomarkers for the diagnosis of OSCC, as well as for treatment strategies.