The genus Strobilurus is prevalent in the Himalayan temperate forests of Pakistan, with sporocarps collected from the Hindukush region. Extensive field surveys spanning five years (2018-2022) were conducted in these locales, resulting in multiple collections of the present specimen. Identification of the specimen was accomplished through comprehensive morphological, anatomical, and molecular analyses. The primary macro- and micro-morphological characteristics of the Pakistani collection include a flat pileus with a rimose margin, varying from yellow to dark-yellow in color, free lamellae, strigos, smooth and reddish stipe, ovoid to ellipsoid basidiospores with a rounded apex, guttulate basidia bearing 2-3 sharp sterigmata, fusoid-ampullaceous cheilocystidia, fusoid-ventricose pleurocystidia, conical and thick-walled hyaline pilipellis, and branched and gutulllated stipitipellis. Morphological, molecular, and phylogenetic analyses collectively confirm the specimen’s identity as Strobilurus albipilatus, marking a new record for Pakistan.