Rabies is a fatal viral zoonotic disease that is maintained in certain wildlife populations in South Africa, and is associated with spillover events between wildlife, domestic animals, and humans. Control efforts consist primarily of mass vaccination drives targeting domestic dogs, which stem from the One Health intersectoral collaboration including humans, animals, and the environment in mitigation approaches. The risk of human rabies infection is an important public health concern, given that the human encroachment on previously unused, privately owned land is increasing. With the rewilding of the Addo-to-Fish Biodiversity Corridor between Addo Elephant National Park and the Great Fish River Nature Reserve, previously isolated wildlife populations are now interacting with each other and the communities that line these borders. We conducted a survey (n = 56) in areas around the Addo to Fish Biodiversity Corridor focused on townships that are within or share a border with game reserves, or those that are further away from game reserve borders. The survey was designed for households with pets and/or livestock. Survey results identified a need for education on rabies and access to the rabies vaccine for their pets, with an overall 67% vaccination rate reported among households sampled. Although slightly below the World Health Organization recommendation of a minimum 70% coverage, the overall vaccination rate demonstrates great coverage from private veterinary clinics in the region. In rural areas within and nearer to private reserves, dogs were under vaccinated and included households not highly educated, thus representing increased risk for contracting rabies. More resources are necessary to reach the 70% vaccination rate per township, and dog owners should be given more support and educational awareness in understanding the clinical signs associated with rabies virus infection in animals and humans.