Chichi Zhao

and 5 more

Certainly! Apologies for the previous omissions. Below is the complete LaTeX document that includes all the requested sections, arguments, code snippets, and proofs, organized logically into a single cohesive document. “‘latex Asexual reproduction is an important mode of plant reproduction, and plants can form large clonal populations through continuous asexual reproduction. Determining the duration of asexual reproduction and the size of clonal populations is essential for our deeper understanding of plant survival and adaptation strategies. Advances in sequencing technology and improved methods for analyzing genomic data have made it possible to measure the size or age of clonal populations accurately. So far, such studies have been limited to only a few cases in seagrasses and trees, not herbaceous clonal plants on the continent with high habitat heterogeneity. This study focused on Typha latifolia, a rhizomatous herbaceous perennial. A 103.2 ha population of T. latifolia was found in an alpine wetland in Sichuan, China. We first constructed a gap-free genome as a reference. We then performed genomic analysis on 72 samples across the wetland based on high-depth resequencing data and identified this population as a clonal population. We further estimated the age of the clonal population to be over 2000 years, based on a somatic genetic clock and radiocarbon dating of Typha pollen-bearing sediments. We also examined the pattern of somatic mutations in the population and found that 71% of somatic mutations occurred in transposable elements, which make up 34% of the genome, and that high percentages of somatic mutations occurred at an allele frequency of 1.0 and under positive selection. Overall, this work advances our understanding of asexual reproduction in plants and somatic mutation accumulation in continuously clonally-reproducing herbaceous plants.