Abstract
Certainly! Apologies for the previous omissions. Below is the complete LaTeX document that includes all the requested sections, arguments, code snippets, and proofs, organized logically into a single cohesive document.
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Asexual reproduction is an important mode of plant reproduction, and
plants can form large clonal populations through continuous asexual
reproduction. Determining the duration of asexual reproduction and the
size of clonal populations is essential for our deeper understanding of
plant survival and adaptation strategies. Advances in sequencing
technology and improved methods for analyzing genomic data have made it
possible to measure the size or age of clonal populations accurately. So
far, such studies have been limited to only a few cases in seagrasses
and trees, not herbaceous clonal plants on the continent with high
habitat heterogeneity. This study focused on Typha latifolia, a
rhizomatous herbaceous perennial. A 103.2 ha population of T.
latifolia was found in an alpine wetland in Sichuan, China. We first
constructed a gap-free genome as a reference. We then performed genomic
analysis on 72 samples across the wetland based on high-depth
resequencing data and identified this population as a clonal population.
We further estimated the age of the clonal population to be over 2000
years, based on a somatic genetic clock and radiocarbon dating of
Typha pollen-bearing sediments. We also examined the pattern of
somatic mutations in the population and found that 71% of somatic
mutations occurred in transposable elements, which make up 34% of the
genome, and that high percentages of somatic mutations occurred at an
allele frequency of 1.0 and under positive selection. Overall, this work
advances our understanding of asexual reproduction in plants and somatic
mutation accumulation in continuously clonally-reproducing herbaceous
plants.