Background It is important to examine the effects of quality of life and determine the cost of illness of Acute Myocardial Infarction, one of the leading causes of death worldwide. factors, and comorbidities by this prospective study. Methods We calculated the cost of illness according to the bottom-up costing approach from the perspectives of patient and relatives, payer, and societal, and evaluated the disease-specific quality of life with HeartQoL and the general quality of life with EQ-5D-5L. Results The mean HeartQoL global score is 2.13±0.73. The mean EuroQol index score of the patients is 0.84±0.24, and the mean EuroQol VAS score is 70.74±22.94. The cost of illness per patient is $489.34 from the patient and relatives perspective, $641.98 from the payer perspective, and $1,131.32 from the societal perspective. The general quality of life scores of those without comorbidities are higher than those with comorbidities. Conclusion We concluded that the quality of life of patients with a family history of hypertension, diabetes, cholesterol, and obesity is lower than patients without any. It has been determined that the cost of illness is higher in patients with risk factors, and loss of income, medical supplies, and direct medical care costs have an important place in the cost of Acute Myocardial Infarction. We concluded that the quality of life of patients with low cost of illness compared better to patients with high cost of illness.