Background: Globally, 29% of under-five mortalities were due to poor vaccine cold chain management and poor immunization uptake, as reported by WHO in 2017. Objective: This study aimed to assess the factors affecting vaccine cold chain management practices in public health facilities in Hargeisa, Somaliland. Methods: This study used a cross-sectional survey, using purposive sampling, of 42 public health facilities in the Hargeisa district. Logistic regression analysis was performed using STATA. Results: Most respondents (71.4%) of the total sample are females and individuals aged 26-35 (71.4%). The vaccine cold chain management practices among respondents indicated that most public health facilities had poor practices (54.76%). The experience of vaccine handlers significantly affected cold chain management practices with an adjusted odds ratio (AOR 0.06, p = 0.025) and supportive supervision (AOR 20.31, p = 0.004). Education also significantly affected vaccine cold chain management practice (AOR 20.54 p-value of 0.044). Conclusion: the study concludes that enhancing the experience, training, and supportive supervision of vaccine handlers, along with promoting advanced education opportunities for diploma holders, is essential for improving vaccine cold chain management practices in public health facilities in Hargeisa, Somaliland.