Stress can activate physiological changes mediated by the sympathetic nervous system and the hypotalamic-pituitary-adrenal axis, triggering the release of biomarkers such as cortisol and proinflammatory cytokines. Although physiological stress has been studied in relation to different inducers and diseases, there is still a gap regarding the association of academic stress with biological markers. Thus, this study aimed to associate the levels of academic stress against biological markers isolated from saliva from undergraduates’ students. Eighty-one students (53 females and 28 males) were recruited and completed the SISCO inventory to determine their academic stress level. The levels of cortisol, interleukin-1β, C-reactive protein and immunoglobulin A from saliva samples were determined by ELISA assays, and data were analyzed using ANOVA, Pearson correlation tests. A predictor model was estimated by lineal regression.Stress categorization following the SISCO inventory showed that 37% of the students grouped in the low stress level, 35% grouped in the moderate stress level , and 28% in high stress level. Salivary marker levels were similar across all stress categories; however, trends identified-such as decreased cortisol and increased proinflammatory markers in male participants classified in the high stress group, suggesting a possible association between these biomarkers and sex-dependent academic stress. The multivariable model including the 4 biomarkers resulted in R2 = 0.14 with predictions that were roughly within +/- 20% of stress levels. In conclusion, no significance was found in the association of salivary biomarkers with academic stress levels. However, trends were observed with increasing levels of academic stress in men.