Many factors affect the fatigue life of materials, including environmental factors and type of loading. The load application frequency is effective as one of the characteristics of loading in the fatigue phenomenon, and in this article, the effect of load application frequency on the fatigue life of aluminum alloy 1100 in two environments, vacuum and atmosphere, is investigated. The fatigue life of the mentioned alloy is evaluated in the areas away from the resonance frequency in both atmosphere and vacuum environments, and a relationship has been proposed to predict the fatigue life according to the load application frequency.