Real-time PCR has been used in clinical diagnostics as the gold standard to detect monkeypox virus (MPXV), which causes monkeypox disease (mpox). However, to better prevent the spread of mpox disease, fast and self-administrated detection medical devices are urgently needed. In this study, we employed loop-mediated isothermal amplification (LAMP) to detect mpox using lyophilized microspheres and read the results with color change and lateral flow immunoassay strips. We first tested the optimal temperature for our LAMP assay and found that 65°C gave the best results. We then used this temperature and tested three different primer groups targeting different regions of the MPXV genes and found that the F3L-1 primer outperformed the other two with a limit of detection (LoD) of 70 genome copies per reaction. Adding another primer group to the F3L-1 group only lowered the LoD to 60 genome copies per reaction. For interference assays, we chose substances and/or microorganisms that are commonly applied to or found on patients’ skins and showed that none of them interfered with the testing results at the indicated concentrations. Finally, we tested clinically collected human skin swabs containing MPXV viruses and demonstrated that our assay performed well with these samples at 1x, 2x, 3x, and 5x LoD. Based on these findings, we proposed a medical device that can be used as a point-of-care solution in areas where traditional PCR equipment is not available.