Silver nanoparticles (AgNPs) have garnered significant attention due to their unique properties and wide range of applications. The green synthesis of AgNPs using plant extracts is an eco-friendly and cost-effective approach that has been extensively studied. This study presents the first-time synthesis and evaluation of silver nanoparticles (AgNPs) using black rosehip ( Rosa pimpinellifolia L.) seed aqueous extract as a potential pharmacological agent. The green synthesis approach yielded spherical Rp-AgNPs with dimensions between 5-200 nm and an average size of 51.93 nm, confirmed by various characterization techniques including UV-Vis spectroscopy, FTIR, TEM, SEM, XRD, DSC, Raman spectroscopy, and XPS analysis. The Rp-AgNPs demonstrated significant antioxidant, cytotoxic, and antimicrobial properties. Their antioxidant capacity surpassed that of the seed extract alone, with a lower IC 50 value in the DPPH assay. The nanoparticles exhibited cytotoxicity against H460 non-small cell lung cancer cells, with an IC 50 of 65.8 μg/mL. Antimicrobial activity was observed against both bacteria and fungi, with inhibition zones ranging from 10-14 mm for bacteria and 7-8 mm for Candida strains. Minimum Inhibitory Concentration values ranged from 50-100 μg/mL for bacteria and 100-200 μg/mL for Candida. This comprehensive study highlights the potential of black rosehip seed extract-mediated AgNPs for various pharmacological applications, especially antioxidant, anticancer and antimicrobial agents, paving the way for further exploration and development in the field of green nanotechnology for biomedical applications.