Probiotic bacteria are playing a pivotal role in the natural treatment of gastrointestinal disorders including diarrhea, constipation, lactose intolerance, some bowel cancers. Beneficial effects are mostly attributed to postbiotic metabolites produced by probiotics, and the unique biological characteristics may vary depending on their species and source. Present study focused on identifying unique Lacticaseibacillus paracasei strains with unique probiotic and anticancer effects originating from fermented pickles. Within the scope of the study, bacterial strains were first isolated and bacterial identification was conducted by 16S rRNA sequence analysis. In vitro environments simulating the gastrointestinal tract were created using the pancreatin and different concentrations of pepsin and bile. Subsequently, the probiotic potentials of Lacticaseibacillus paracasei strains was determined by testing their viability rates in the environments simulated. The adhesive capabilities of isolates on L-929, HT-29, and Caco-2 cell lines were evaluated. Furthermore, extracellular postbiotic metabolites (PM) and bacterial extracts (BE) were prepared and the cytotoxic effects of the PM and BE on cell lines were explored. Alongside the considerable impact in bacterial adhesion, all strains, primarily LP001, displayed generally acceptable biological activities. It is also clear that PM promises a more acceptable cell viability rate than BE samples.