Objectives: The objective of this study was to investigate the impact of acetylcholine signaling pathways on tumor progression, focusing on the activation of nicotinic acetylcholine receptors in breast, prostate, and lung cancer cell lines. Methods: We examined the production and release of acetylcholine in the central nervous system and its potential effects on tumors in the peripheral environment. Utilizing breast, prostate, and lung cancer cell lines, we explored the signaling pathways associated with the activation of nicotinic acetylcholine receptors. Results: Our study revealed insights into the modulation of specific tumor profiling signaling pathways through the activation of nicotinic acetylcholine receptors. We observed notable implications for patient well-being and mortality rates based on the manipulation of these pathways. Conclusion: The findings from this investigation provide valuable information on the intricate relationship between acetylcholine signaling and tumor progression. By elucidating these pathways, there is potential for targeted interventions to enhance patient outcomes and mitigate mortality rates in breast, prostate, and lung cancer cases.