not-yet-known not-yet-known not-yet-known unknown Introduction: Understand the drug-resistant situation and trend of tuberculosis patients in Yuexiu District, Guangzhou City, from 2013 to 2022, provide a scientific basis for development of rational drug-resistant tuberculosis prevention and control strategies. Methods: All patients who were diagnosed with active tuberculosis in Guangzhou Chest Hospital from January 1, 2013 to December 31, 2022 were collected as study subjects, a total of 5,191 patients were enrolled in the study. The basic conditions and diagnostic and therapeutic information of the study subjects were collected. Sputum specimens were subjected to smear and isolation and culture. Culture-positive strains of bacteria were identified by bacterial groups. A total of 1,659 strains of Mycobacterium tuberculosis (MTB) isolates were obtained. The drug susceptibility test was used the proportionality method on the MTB isolates for nine types of anti-tuberculosis medicines,and comparatively analyzed the resistance of the strains. Results: A total of 1,659 patients with MTB were cultured, 439 were drug-resistant, 255 were mono-resistant, and 121 were poly-resistant, 62 were multidrug-resistant. The resistance rate was 26.46% (439/1659), the mono-resistance rate was 15.37 % (255/1659), and the poly-resistance rate was 7.29% (121/1659), the multidrug resistance rate was 3.74 % (62/1659) . The number (rate) of drug-resistant patients in the primary treatment was 389 [(25.63%).(389/1659）].The number (rate)of drug-resistant patients in retreatment was 50[ (35.46%) (50/1659）]. The number (rate) of multidrug-resistant patients in the primary treatment was 39 (2.57%)[(39/1659）]. The number (rate) of multidrug-resistant cases in retreatment patients was 23 [(16.31%)(23/1659）]. The resistance rate and multidrug resistance rate of isolates from retreatment patients were higher than those of isolates from primary treatment patients, and the differences were statistically significant. Conclusions: The problem of drug-resistant tuberculosis transmission in Guangzhou needs to be focused on, and drug-resistant screening should be further increased to do a good job of controlling the source of infection.