The report discusses the implications of using different volumes of flushing media during embryo flushing (EF) procedures in a single donor mare that experienced multiple unintended pregnancies following negative EF outcomes. The study involved two sets of EF experiments over two years on a non-lactating Spanish purebred mare with a single functioning ovary. The initial experiments (group, n=6) used 1L of flushing medium (Ringer´s Lactate), and subsequent tests (group 2, n=5) increased the volume to 2L, to assess whether a larger volume could enhance embryo recovery rates. No luteolytic agent was administered after any of the EF procedures. Data collection included the number of double and single ovulations (OV), embryos recovered and subsequent pregnancies. The overall incidence of double OV was 36.3%, and did not vary (p>0.05) between group 1 and 2 (33.3% and 40%). The results indicated a significant improvement (p<0.05) in embryo recovery rates with the increased medium volume in group 1 and 2, (25% and 85.7%, respectively). A tendency was noted (p=0.06) for more positive EF outcomes in group 2 (100% positive outcome). The number of pregnancies found based on the number of OV recorded was significant (4/8 and 0/7, p<0.05). The study concluded that a higher volume of flushing medium yields a higher embryo recovery rate and minimizes unintended pregnancies in the subject mare.