The cortical somatostatin interneuron population includes several diverse cell types, among them the Martinotti cells. Layer-specific differences in connectivity and function between different subtypes of Martinotti cells are becoming apparent, which require contemporary studies to investigate cortical interneurons in a layer and subtype specific manner. In this study, we investigate the connectivity of a subtype of Chrna2+ layer 5 Martinotti cells in the primary motor cortex, using a monosynaptic retrograde rabies viral tracer. We found direct input from pyramidal cells and local parvalbumin interneurons. In addition, we found long-range direct inputs from the motor thalamus, substantia innominata of the basal forebrain and globus pallidus. Based on the observed input pattern, we tested and found an increased number of falls in the hanging wire test upon temporary overexcitation of Chrna2+ layer 5 Martinotti cells. These results suggest that Chrna2+ Martinotti cells in the motor cortex are involved in sensorimotor integration.