Background: Hepatitis B infection is a significant public health issue both in India and globally. Understanding the seroprevalence among populations and identifying associated risk factors are crucial for early diagnosis, effective treatment, and timely prevention of viral hepatitis and its complications. The viral load is a key predictor of the disease’s prognosis and severity. Objectives: A prospective study was conducted to assess the seroprevalence of HBV and to identify possible risk factors for acquiring HBV infection among the patients attending Dr. B. R.A. M. Hospital, Raipur and to estimate the viral load in seropositive individuals. Methods: Blood samples were collected over the duration of 14 months October 2022-December 2023 from the patients attending OPDs of Dr .B. R. A. M. Hospital, Raipur. The serum was subjected to ELISA for HBsAg detection. Viral load quantification was done by Mylab PathoDetect HBV Quantitative PCR Kit. Detailed history, socio-demographic parameters, information about risk factors were obtained from each of the patient according to pre-designed questionnaire by one to one oral interview. Result: 3002 individuals were screened for HBV infection among them 49(1.63%) were positive for HBsAg. High risk sexual behavior and blood transfusion were the major risk factors observed among the patients. The mean viral load of HBV was 8.533×10 6 ± 3.627×10 7IU/ml. Conclusion: The rising prevalence of HBV in our region is a public health concern. Preventive measures must be implemented to cease the disease spread.