Bioinformatic analyses have become an integral part of modern life sciences. This manuscript is an ideal introduction into the exciting world of transcriptome analysis and is aimed at beginners. For example, as a comprehensible introduction for students, or as a useful addition to school lessons. Bioinformaticians deal with the research of genomes, i.e. the collection of blueprints of living organisms. For a few decades, they have been able to read the text of the individual blueprints, the transcripts, using sequencing methods. Over time, more and more sequencing methods have been developed and, for around 20 years, a method called RNA-Sequencing (RNA-Seq) has even made it possible to determine how often individual blueprints are read and required by a living organism. The combination of sequence information with information on the frequency of use enables an enormous number of insights into the function of the organism. Since RNASeq generates huge amounts of data, the work of bioinformaticians is similar to that of gold miners, with an extensive search for the relevant information. In this article you will find out how modern gold miners work and what the gold nuggets they are looking for look like.