Advanced sequencing technologies are becoming more accessible due to decreasing costs. However, these technologies require strict adherence to standards regarding the amount of DNA input and its integrity. This study addresses the challenge of obtaining high-quality host DNA in sufficient quantities from small biomass and multiple DNA source arthropods, using the tick species Amblyomma triguttatum as a model organism. We evaluated different tissue types and disruption methods to optimise DNA yield, considering quantity, quality, and composition of purified genomic DNA (gDNA). Our factorial experiment included three types of tissue (Whole and Bisected specimens, and specimen Legs) and three levels of disruption methods (Undisrupted, Sliced, and liquid nitrogen bead Homogenisation). We also contrasted two extraction kits. Results showed using the Qiagen MagAttract High Molecular Weight Kit significantly increased the proportion of high molecular weight fragments (20-48.5 kbp) by 11-fold, compared to the Qiagen DNeasy Blood & Tissue Kit. Aggressive homogenisation techniques produced the highest proportion of short fragments (1-10 kbp) at 97% (0.970). While Whole-Homogenised specimens yielded the highest DNA concentration (198 ng µL-1), Bisected-Undisrupted specimens offered a good balance, yielding 146 ng µL-1 of gDNA with a higher proportion of large fragments at 3.15% (0.0315). Bacterial content in DNA did not vary significantly across treatments. Our findings highlight the importance of selecting appropriate extraction methods to ensure optimal DNA quality for advanced sequencing applications. These results provide useful guidelines for optimising DNA extractions from small-bodied arthropods and establish a framework for future studies to consider DNA quantity, quality, and composition.