Equine coital rash (ECE) is a highly contagious benign disease that induces lesions on the external genitals, and it is caused by the equine herpesvirus type 3 (EHV-3). The disease is globally distributed and affects only equids. However, its presence in Chile has not been documented from a genetic point of view. Here, we performed PCR screenings for EHV-3 in genital lesions of external genitals in four horses belonging to a riding station at Bulnes, Ñuble Region, Chile. We sequenced a fragment of the glycoprotein g ( gG) gene of this alphaherpesvirus, from three horses which signology compatible with ECE. The sequences were identical between them and 99.7% similar to a haplotype of EHV-3 detected in Brazil. A phylogenetic analysis pointed that our consensus sequence forms a clade with homologue isolates obtained from Japan, Russia and Brazil. Our results show the presence of EHV-3 for the first time in horses with ECE in Chile.