Aim: Present study is design to investigate the effect of hydroalcoholic extract of Centella asiatica (HA-CA) in 3-nitro propionic acid induced Huntington’s disease in adult zebrafish. Material and method: In current study, adult zebrafish (approximately 5-6 months old), weighing from 470-530 mg were used and treated with 3-NP (5 mg/kg i.p). The animals received HA-CA (80, and 100 mg/l) daily up to twenty eight days in water. Tetrabenazine (TBZ) (3 mg/kg i.p.) was used as standard drug. The body weight and behavior were performed before 3-NP administration on every 4th day. Then animal were sacrificed for biochemical analysis using UV-spectrophotometer. Inflammatory markers were measured by using zebrafish ELISA kits. Neuronal damage in the brain was examined by using Hematoxylin and eosin (H&E) staining. Results: 3-NP treated zebrafish showed marked reduction in body weight, cognition, and locomotion activity accompanied by progressive striatal dysfunction. HA-CA treated group significantly reversed 3-NP induced increase level of LPO, AChEs, Nitrite, and decrease level of GSH. Administration of HA-CA (80, 100 mg/l) significantly ameliorates 3-NP induced alteration in body weight and behavior, restored antioxidant enzymes level, decrease ROS production and neuroinflammation. HA-CA significantly decreases TNF-α, and IL-1β levels in the brain. Moreover, HA-CA significantly reversed 3-NP induced neuronal loss in the brain. Conclusion: HA-CA exhibits protective effect against 3-NP induced decrease locomotor activity, increase in oxidative stress, neuroinflammation, and neuronal loss in HD model of zebrafish, which is associated with its antioxidant activity. Keywords: 3-nitropropionic acid, Centella asiatica, oxidative stress, neurotoxicity, neuroinflammation, zebrafish