Purpose: To explore the value of metagenomics next-generation sequencing (mNGS) for deep sternal wound infection (DSWI) diagnosis Method: The mNGS was used to diagnose the DSWI after cardiac transplantation and guide the antibiotics prescription Results: The co-infection of methicillin-resistant Staphylococcus aureus (MRSA) and cytomegalovirus (CMV) was detected in this patient. The infection was controlled and the wound healed successfully with the specific medicine based on mNGS results for three weeks. Conclusion: The mNGS is effective to achieve precise, individualized, and rapid treatment for wound infection