Ascorbic acid (AA) is an important deoxidizer contained in food, drink and medicine. However, existing detection methods are time-consuming, expensive and complex in the industrial production and quality testing. In order to address above issue, we proposed a core-shell nanowires constructed non-enzymatic sensor to achieve the highly sensitive and selective recognition of AA with a long-term reusability. The core-shell architecture was composed of Cu-Fe Prussian blue of 10 nm thickness covering 30 nm Cu nanowire. This nanocomposite couples advances of Cu and Prussian blue to possess both high electrocatalysis and electron transfer rate. The as-prepared AA sensor shows an excellent sensitivity with an ultrawide linear range from 0.01 to 3.5 mM, together with the remarkable anti-interference and reusable ability beyond 30 days. Used for four kinds of juice and bear as real samples, this sensor had accurately and rapidly reported the AA concentration with a good recovery.