Fluid shear stress (FSS) has been acknowledged as an important cue in cancer development and growth. Cancer cell stemness is known to change with FSS signal. However it is not clear how the stemness changes with different orders of FSS magnitude. HeLa cancer cell proliferation, intracellular reactive oxygen species and stemness were analysed with different assays like flow cytometer analysis, RT-pcr, immunocytochemistry etc. in a logarithmic microfluidic device with wide physiological FSS. Less number of cells in G0/G1 phase was detected after FSS treatment, indicating enhanced cell proliferation or S/G2/M phase arrest. EdU staining revealed medium to low FSS increased cell proliferation whereas high shear reduced it. FSS also increased capability to withstand higher intracellular ROS and mitochondrial membrane potential in HeLa. We could see increment in stemness characteristics of cancer cells in magnitude dependent manner through gene expression (Sox2, N-cadherin, Vimentin), cell surface markers (CD44, CD33, CD117) and colony forming ability analysis. This increment trend was also found in drug resistance capability of HeLa to doxorubicin after FSS treatment. These data indicates increase in stemness and drug resistance characteristics of cancer cells with FSS magnitude which is experienced during metastasis and in native tissues.