The global SARS-CoV-2 pandemic has required a rapid and reliable diagnostic test. RT-qPCR is currently the gold standard method for standard SARS-CoV-2 detection. Here we developed a protocol based on RT-LAMP and we compare the results obtained in fresh RNA extracts and in RNA samples with some freezing/thawing cycles, with those obtained using the standard method. We demonstrated that RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct value between 0-35