Background and Purpose:The skin is an important barrier to protect the body from external damage, there are tens of millions of patients with various skin defects. Due to the complex process of wound repair, it is easy to cause poor healing and other problems. Wound healing procedures have a systematic multi-stage: hemostasis, inflammation, proliferation and remodeling. Here, we prepared a new bio- responsive transdermal oil body microgel emulsion (OBEME) for drug delivery carrier. Experimental Approach: OBEME was stored at room temperature for 90 days, and its stability was evaluated by low speed centrifugation and repeated freeze-thaw treatment. The transdermal penetration of OBEME was proved by transdermal administration, and the effect of OBEME on wound healing was investigated in the model of full-thickness skin defect. Key Results: OBEME was stably stored at room temperature for 90 days and is not affected by low-speed centrifugation and repeated freeze-thaw. It was proved to have a good sustained release effect through percutaneous administration. The OBEME significantly promoted wound healing, regulated inflammatory response, and accelerated angiogenesis, re-epithelialization and remodeling. Conclusion and Implications: Taken together, the OBEME is a good new carrier for percutaneous drug delivery that can promote wound healing and it has a good sustained-release effect and can induce the correct repair of skin, which lays a foundation for the development of new topical drugs.