Background: Standard precautions (SPs) practices are of crucial management skills among Health Care Workers (HCWs) including nurses against the highly infectious novel virus SARS-CoV-2. Aim of the study: The study aimed to assess level of nurse’s compliance with SPs, and identify the main challenges that experienced by nurses during their work with infected SARS-CoV-2 patient’s. Methods: A cross-sectional survey design was used. The study was conducted in different sectors that represent the health care sector in Jordan. 386 nurses recruited from the different sectors. Results: Data were collected from 386 participants. 66.8% of the participants were female. Most of them revealed that they were dealt previously SARS-CoV-2 patients (73.6%). Generally, nurses demonstrated good level of compliance with SPs practices (71%). The staff reported that they mostly adhere with performing hand hygiene after all procedures (65.8%). On the other hand, they were least adherence with maintaining a physical distance of patient and staff of at least 6ft apart (28.5%). conclusion: It was concluded that nurses have a good level of SPs practices. Many challenges were experienced by nurses while caring of SARS-CoV-2 patients.