Our aim was to derive male and female reference limits of left-ventricular functional parameters for patients with low-likehood coronary heart disease using three quantitative methods and determined the effect of gender, age and body weight on cardiac functional parameters. One hundred and seventy-five consecutive patients who defined as low-likehood coronary heart disease patients underwent adenosine or exercise stress 99mTc-MIBI gated myocardial perfusion single photon emission computed tomography (SPECT) imaging, left ventricular (LV) ejection fraction (EF)，end-diastolic volume (EDV)，and end-systolic volume (ESV) were obtained by QGS, ECToolbox and 4D-MSPECT, respectively. LVEF and LV volumes determined by gated SPECT are significantly affected by gender and age, with body weight as a significant predictor for cardiac volumes. The values of EDV and EF determined by three methods could be different although they correlate very well; the reference limit of EDV, ESV and EF calculated by three methods are established.