More than fifty years of conflict in Colombia has left a country with many needs. This article analyzes how Science, Technology, and Innovation (STI) can contribute to improving communities affected by violence in a post-conflict stage. This paper begins with an introduction focused on the processes of the generation of violence and the peace agreement, following the role of the UN in the post-conflict stage. A conceptual analysis of the actors involved in Science, Technology, and Innovation activities is also presented with one analysis of the activities of STI that can contribute to the post-conflict, continuing with some financing strategies for the activities of STI and a brief description of the legal framework STI against the post-conflict. The paper concludes with some recommendations and opportunities in this new era for the country.