*Correspondence: AAAnikin@kantiana.ruAbstractThe work is devoted to the magnetic layered microdisks synthesized by tear-off optical lithography combined with electron-beam deposition of metal layers that are considered to be the microagents for the anti-cancer treatments. Two types of microdisks were studied with different layers sequences with thicknesses in nm: Au(10)/Fe(70)/Au(10) and Fe(50)/Au(10)/Fe(50). First type of disks showed slightly higher optical absorption and lower magnetic susceptibility, the photothermal conversion coefficient is 27%. The second type showed higher magnetic susceptibility and the photothermal conversion coefficient is 30%. The single photothermal and magnetomechanical therapies conducted with the microdisks showed mild decrease of cell viability. For the photothermal therapy ~10% cells viability decrease was achieved. For the magnetomechanical therapy it reached ~17% cells viability decrease. However, if photothermal therapy is conducted after the magnetomechanical stimulation the viability decreases by ~30%. In combinational treatment with simultaneous photothermal and magnetomechanical impact the viability decreased by 50%.1. Introduction Magnetic particles (MPs) find wide application in biomedical field, including cancer treatment using magnetic hyperthermia [\cite{Kafrouni2016}], or magnetically controlled delivery and release of antitumor drugs to the target area [\cite{Oliveira2013}]. Recently, methods of cancer cell destruction based on movement of magnetic particles in changing magnetic fields have been actively developed [\cite{Naud2020}, \cite{Golovin_2018}]. The most suitable materials should have high mechanical moment under the influence of changing magnetic fields. In particular, microdisks with vortex magnetic structure in the ground state, created using magnetron sputtering and subsequent optical lithography [\cite{Kilinc_2016},\cite{therapy}], nanowires and nanotubes obtained by electrochemical synthesis in porous membranes [\cite{Contreras_2015},\cite{Anikin_2024}], ultrathin magnetic particles with perpendicular magnetization [\cite{Mansell_2017}], as well as synthetic antiferromagnetic microdisks with zero residual magnetization are considered. The main difficulties in preparing such materials arise in obtaining stable colloidal solutions of nanoparticles with their optimal magnetomechanical and hysteresis properties, in reducing cytotoxicity, and in scaling up the synthesis methods.Photothermal and magnetomechanical therapies with nanoparticles are among novel approaches to cancer treatment [\cite{Ali_2019}, \cite{Vines_2019}, \cite{Naud_2020}, \cite{Subramanian_2019}]. Photothermal therapy (PTT) involves heating by incident light of cancer cells loaded with plasmonic nanoparticles that achieves the temperature required for cell destruction. Magnetomechanical therapy (MMT) can be divided into magnetic drug delivery and magnetomechanical cell destruction, both requiring the use of magnetic nanoparticles.Using only one method of influencing cells may not be effective enough. Of interest is the study of the combined action of mechanical forces and heating, both due to magnetic hyperthermia and photothermia [\cite{Efremova_2018}, \cite{Shi_2022},\cite{Espinosa_2018}]. However, magnetic materials, as a rule, do not heat up well enough when interacting with light, especially in the near IR range, at wavelengths of about 800 nm, which is the first transparency window of biological tissues. On the other hand, in order to improve the biocompatibility and stability of MPs, as well as to reduce their agglomeration, coatings of various biocompatible materials, including gold, are used [\cite{Subekin_2024}]. This leads to an increase in optical absorption and the efficiency of photothermal heating, which makes it possible to combine several anticancer therapies using one type of particles. This is especially relevant in the context of the emerging field of nanomedicine, where such particles can be used to generate local forces or moments on biological samples at different temperatures and study the cellular response.In this work, we investigated the optical, photothermal and magnetic properties of layered microdisks in two configurations, Au/Fe/Au (Au/Fe/Au) and Fe/Au/Fe (Fe/Au/Fe), fabricated by lift-off optical lithography. The first type of discs has high optical absorption up to the IR region and photothermal efficiency. While the second type has greater potential for use in magnetomechanical therapy. Single photothermal and magnetomechanical therapies were performed, as well as combined therapies. The results show potential of the biomedical use of the microdisks.

Composite nanoparticles with a gold core enveloped by cobalt ferrite nanoparticles show potential for enhanced photothermal therapy. Determining the optimal gold-to-cobalt ferrite nanoparticle ratio, dependent on size, is vital for improving treatment efficiency. We address the urgent need for advancing photothermal therapy through utilising combined plasmon-magnetic composites with potential of controlled directional delivery. Our computational modeling and experimental absorption spectra analysis reveal that adjusting the cobalt ferrite nanoparticle content redshifts the plasmon resonance frequency in gold nanoparticles, which is accompanied by increase in the extinction cross-section. As a result, cobalt ferrite nanoparticle absorption dominates. Our experiments on photothermal response in aqueous solutions of composite nanoparticles of various concentrations demonstrate that 100 μg/ml solution yields a significant temperature increase of ~8.2 K and a photothermal conversion efficiency of ~51%. At this concentration, the composite nanoparticles effectively heat the cell culture medium under photothermal conditions, leading to 22% reduction in cell viability.