In recent years, how to inhibit the proliferation of tumor cells and the expression of stem genes has become one of the focuses of scientists in treating cancer. Cancer stem cell (CSC) is a kind of cell group with self-renewal ability in tumors, and is considered as one of the driving factors in the process of tumorigenesis. The Hippo signaling pathway is a highly conserved signaling pathway in biological evolution that plays an important role in cell proliferation, tissue development, and organ size. In this article, Hippo signaling pathway and its mechanism in Cancer stem cell were briefly reviewed, and the prospect of tumor treatment was proposed.