Viral diseases are the primary cause of disease and economic loss in all bird species. Among viral diseases, Newcastle disease (ND) and Avian Influenza (AI) have a very special place due to their high morbidity and high mortality. They inflict lots of deaths and economic losses upon poultry and nonpoultry avian species, making them a hot subject in various research. Thus, prevention measures and strategies to control the mentioned diseases are wide. Vaccination is one of the common methods. In this study we designed a vaccination protocol based on previous and popular methods to evaluate the effect of ND and AI vaccines on humoral immune response of 14 bird species. We tested 115 birds (sampled twice) with no previous encounter to disease or vaccine. Birds were vaccinated with NDV B1 strain and a bivalent inactivated ND+AI, and repeated 5 weeks later. HI was used for humoral response evaluation and implemented 5 weeks post each vaccination. Our results showed a double vaccination of ND is can boost immune system significantly, but a single AI vaccination will provide enough protection, since the second vaccination did not change the titers significantly, even lowered titer in one case. There were some discrepancies in results which further studies should be accomplished to elaborate the details.